ID B8ENC5_METSB Unreviewed; 523 AA.
AC B8ENC5;
DT 03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 03-MAR-2009, sequence version 1.
DT 27-MAR-2024, entry version 64.
DE RecName: Full=tRNA threonylcarbamoyladenosine biosynthesis protein TsaE {ECO:0000256|ARBA:ARBA00019010};
DE AltName: Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE {ECO:0000256|ARBA:ARBA00032441};
GN OrderedLocusNames=Msil_0666 {ECO:0000313|EMBL:ACK49638.1};
OS Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB
OS 13906 / BL2).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Beijerinckiaceae; Methylocella.
OX NCBI_TaxID=395965 {ECO:0000313|EMBL:ACK49638.1, ECO:0000313|Proteomes:UP000002257};
RN [1] {ECO:0000313|EMBL:ACK49638.1, ECO:0000313|Proteomes:UP000002257}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2
RC {ECO:0000313|Proteomes:UP000002257};
RX PubMed=20472789; DOI=10.1128/JB.00506-10;
RA Chen Y., Crombie A., Rahman M.T., Dedysh S.N., Liesack W., Stott M.B.,
RA Alam M., Theisen A.R., Murrell J.C., Dunfield P.F.;
RT "Complete genome sequence of the aerobic facultative methanotroph
RT Methylocella silvestris BL2.";
RL J. Bacteriol. 192:3840-3841(2010).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the TsaE family.
CC {ECO:0000256|ARBA:ARBA00007599}.
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DR EMBL; CP001280; ACK49638.1; -; Genomic_DNA.
DR RefSeq; WP_012589708.1; NC_011666.1.
DR AlphaFoldDB; B8ENC5; -.
DR STRING; 395965.Msil_0666; -.
DR KEGG; msl:Msil_0666; -.
DR eggNOG; COG0802; Bacteria.
DR eggNOG; COG3178; Bacteria.
DR HOGENOM; CLU_021467_2_1_5; -.
DR OrthoDB; 9809275at2; -.
DR Proteomes; UP000002257; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0002949; P:tRNA threonylcarbamoyladenosine modification; IEA:InterPro.
DR Gene3D; 3.90.1200.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR002575; Aminoglycoside_PTrfase.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR003442; T6A_TsaE.
DR NCBIfam; TIGR00150; T6A_YjeE; 1.
DR PANTHER; PTHR33540; TRNA THREONYLCARBAMOYLADENOSINE BIOSYNTHESIS PROTEIN TSAE; 1.
DR PANTHER; PTHR33540:SF2; TRNA THREONYLCARBAMOYLADENOSINE BIOSYNTHESIS PROTEIN TSAE; 1.
DR Pfam; PF01636; APH; 1.
DR Pfam; PF02367; TsaE; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000002257};
KW Transferase {ECO:0000256|ARBA:ARBA00022777}.
FT DOMAIN 187..432
FT /note="Aminoglycoside phosphotransferase"
FT /evidence="ECO:0000259|Pfam:PF01636"
SQ SEQUENCE 523 AA; 57266 MW; 8B02EE4CED80D23C CRC64;
MAVEAQLAAT ATVWPRRLAD EDATSRLAAE IAELIRPGDL VTLSGELGAG KTTFARALIR
LLTGDPDLEV PSPTFLLMQI YEGASAPIVH ADFYRLEKPS DLIELGWDEA ADGAFVIVEW
PDRGGAYLGE DRLDISFALD ADSATGARDA TVTGHGAFAG RLARARAIEG VLAPTRWRDA
QRQFMQGDAS SRLFERLVRD DGSSAVLMIS PPRPDGPPIR FGKPYSAIAR LAEDVRPFIA
VGEGLRAEGI SAPEIYAFDL ATGVILVEDL GDEPILIGGE PDFERHAVAT EVLARLHGSD
LPDTLPVPGA APYTIPPYDL DALLIEVELL LDWYAPYRAR LQLSSGAKAT FVNLWRKALT
ESLTTETSWT LRDYHSPNLI WLAAREGLKR VGLVDFQDCV RGHPAYDLVS LLQDARVTVP
DALELKLLGH YARARQEAIA QKAAGGAFAM GDFVRAYALL GAQRATKILG IFARLDARDR
KPHYLAHLPR VEQYLLKNLR HEALAELKIW YETHLPQIFE SAA
//
