ID B8LKQ9_PICSI Unreviewed; 1032 AA.
AC B8LKQ9;
DT 03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 03-MAR-2009, sequence version 1.
DT 27-MAR-2024, entry version 51.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
OS Picea sitchensis (Sitka spruce) (Pinus sitchensis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Picea.
OX NCBI_TaxID=3332 {ECO:0000313|EMBL:ABR16239.1};
RN [1] {ECO:0000313|EMBL:ABR16239.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Green portion of the leader tissue
RC {ECO:0000313|EMBL:ABR16239.1};
RA Ralph S.G., Chun H.E., Liao N., Ali J., Reid K., Kolosova N., Cooper N.,
RA Cullis C., Jancsik S., Moore R., Mayo M., Wagner S., Holt R.A.,
RA Jones S.J.M., Marra M.A., Ritland C.E., Ritland K., Bohlmann J.;
RT "Full length cDNA sequences from Sitka Spruce (Picea sitchensis).";
RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; EF676330; ABR16239.1; -; mRNA.
DR AlphaFoldDB; B8LKQ9; -.
DR OMA; KRCNENQ; -.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR GO; GO:0000209; P:protein polyubiquitination; IEA:InterPro.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR044611; E3A/B/C-like.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR PANTHER; PTHR45700; UBIQUITIN-PROTEIN LIGASE E3C; 1.
DR PANTHER; PTHR45700:SF2; UBIQUITIN-PROTEIN LIGASE E3C; 1.
DR Pfam; PF00632; HECT; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50237; HECT; 1.
PE 2: Evidence at transcript level;
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 690..1032
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..25
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 1000
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 1032 AA; 117291 MW; 55BF1F7B3E991B03 CRC64;
MFFSGDPSSR KKVDLGGRSS KERDRQKYLE QTKLAREQRQ RLRQQTLSAQ VIQKCFRGRR
AVAMARSEIR EEFCITFGEY GEQADRNAFN PSSEYLRQLL FFFRANNHGD ILRLVEACKL
LHQSIQVSGN ILDIFTNGDI SSDQAIIQYR IRYFIICCLQ AVHQNREQLK SGPSRLSGSA
DLPVLLLLET VIILTDPMLP WAGSTMDYIL QIYTFSHFRE LILTVQGIQT NIPGSGVSHL
ECLLTRLASS SAQKLYTGHN MQLSFPAQIL SIPFLWKQFP NLKQVVMSQG LWPCYVQQIA
TVLPSLHTLL PPDTSSQLPS HACLLGNVLE VAGFALSRTN CTLQTAMDFA TSARFLLEEI
PHNFKTSNAK NSDYDDDMDV DEDAGQITLS KDLERQIVNA VDLDFLKQLV RFLYGDISGF
GDSTSIEGPS EKEAAAVTAL CAFLHVSITM LPLENVMTGL AYTTELVPTL WHYMKRCHLS
QRWPSMEALA AFFPGDAPGW LLPLSVFCPL YRHMLMIIDN EEFYEQQKPL SLQDVKALVI
ILKEALWQLL WVIPSKTSTP QKPVHSGSNQ KRFPLQSIRE RVSTLAAELL AQLQDWNCRR
EFTSPNDFHA RDAVDGMFYA QAGSENSRAH ELLKRAPFLV PFTNRVQIYT SQLAAARQQN
SFHTPFVRHR IRIRRNRAVE DAFSELNGLT GEELRGMIRV IFVNEFGVEE PGVDGGGIFK
DFMENITKTA FDIQYGLFKE TADHLLYPNP ASGTIHPEHL QYFEFLGKIL GKAMFEGILV
DIPFATFFLS KLKEKHNYLH DLPSLDPELY RSLLSLKHYD GDVSELGLYF VSENNEYGEQ
KEEEMLPGGK DMQVTNENVI KFIHLMANHH LNSQIRNQSS YFLRGFQQLI QSDWLNMFNE
HELQLLISGS TEGMDVDGLR SNVHYAGGYS EDHPVIEMFW EVIKQFSLEQ QKKFLKFVTG
CSRGPLLGFK YLEPPFCIQR AAPEDAPEEV LDRLPTSATC VNLLKLPPYR SKDVLRSKLL
YAISADAGFD LS
//
