ID B8P466_POSPM Unreviewed; 814 AA.
AC B8P466;
DT 03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 03-MAR-2009, sequence version 1.
DT 27-MAR-2024, entry version 55.
DE RecName: Full=tRNA ligase {ECO:0000256|PIRNR:PIRNR019634};
DE EC=6.5.1.3 {ECO:0000256|PIRNR:PIRNR019634};
GN ORFNames=POSPLDRAFT_100403 {ECO:0000313|EMBL:EED84337.1};
OS Postia placenta (strain ATCC 44394 / Madison 698-R) (Brown rot fungus)
OS (Poria monticola).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Polyporales; Postiaceae; Postia.
OX NCBI_TaxID=561896 {ECO:0000313|Proteomes:UP000001743};
RN [1] {ECO:0000313|EMBL:EED84337.1, ECO:0000313|Proteomes:UP000001743}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 44394 / Madison 698-R {ECO:0000313|Proteomes:UP000001743};
RX PubMed=19193860; DOI=10.1073/pnas.0809575106;
RA Martinez D., Challacombe J., Morgenstern I., Hibbett D., Schmoll M.,
RA Kubicek C.P., Ferreira P., Ruiz-Duenas F.J., Martinez A.T., Kersten P.,
RA Hammel K.E., Vanden Wymelenberg A., Gaskell J., Lindquist E., Sabat G.,
RA Splinter BonDurant S., Larrondo L.F., Canessa P., Vicuna R., Yadav J.,
RA Doddapaneni H., Subramanian V., Pisabarro A.G., Lavin J.L., Oguiza J.A.,
RA Master E., Henrissat B., Coutinho P.M., Harris P., Magnuson J.K.,
RA Baker S.E., Bruno K., Kenealy W., Hoegger P.J., Kuees U., Ramaiya P.,
RA Lucas S., Salamov A., Shapiro H., Tu H., Chee C.L., Misra M., Xie G.,
RA Teter S., Yaver D., James T., Mokrejs M., Pospisek M., Grigoriev I.V.,
RA Brettin T., Rokhsar D., Berka R., Cullen D.;
RT "Genome, transcriptome, and secretome analysis of wood decay fungus Postia
RT placenta supports unique mechanisms of lignocellulose conversion.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:1954-1959(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC (ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate.;
CC EC=6.5.1.3; Evidence={ECO:0000256|PIRNR:PIRNR019634};
CC -!- SIMILARITY: Belongs to the TRL1 family.
CC {ECO:0000256|PIRNR:PIRNR019634}.
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DR EMBL; EQ966251; EED84337.1; -; Genomic_DNA.
DR RefSeq; XP_002470448.1; XM_002470403.1.
DR AlphaFoldDB; B8P466; -.
DR KEGG; ppl:POSPLDRAFT_100403; -.
DR HOGENOM; CLU_010316_1_0_1; -.
DR InParanoid; B8P466; -.
DR OMA; FQDWDYK; -.
DR OrthoDB; 1405617at2759; -.
DR Proteomes; UP000001743; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051730; F:GTP-dependent polyribonucleotide 5'-hydroxyl-kinase activity; IEA:InterPro.
DR GO; GO:0008081; F:phosphoric diester hydrolase activity; IEA:InterPro.
DR GO; GO:0003972; F:RNA ligase (ATP) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006388; P:tRNA splicing, via endonucleolytic cleavage and ligation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR019039; T4-Rnl1-like_N.
DR InterPro; IPR012387; Trl1_fun.
DR InterPro; IPR015966; tRNA_lig_kin_fungi.
DR InterPro; IPR015965; tRNA_lig_PDEase.
DR PANTHER; PTHR32004; TRNA LIGASE; 1.
DR PANTHER; PTHR32004:SF1; TRNA LIGASE; 1.
DR Pfam; PF09511; RNA_lig_T4_1; 1.
DR Pfam; PF08302; tRNA_lig_CPD; 1.
DR Pfam; PF08303; tRNA_lig_kinase; 1.
DR PIRSF; PIRSF019634; tRNA_lig_yeast; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000256|PIRNR:PIRNR019634};
KW Reference proteome {ECO:0000313|Proteomes:UP000001743};
KW tRNA processing {ECO:0000256|PIRNR:PIRNR019634}.
FT DOMAIN 80..326
FT /note="T4 RNA ligase 1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF09511"
FT DOMAIN 429..568
FT /note="tRNA ligase kinase"
FT /evidence="ECO:0000259|Pfam:PF08303"
FT DOMAIN 624..811
FT /note="tRNA ligase phosphodiesterase"
FT /evidence="ECO:0000259|Pfam:PF08302"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 133
FT /note="N6-AMP-lysine intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR019634-50"
SQ SEQUENCE 814 AA; 91357 MW; 54BC58BE4721D595 CRC64;
MAALPTSEPA AELEKLSISK HGSEKDSELI DELLALSKKN PKLVRSTEYT APADPNINVR
SWKMNEFKYY DVPSPFPTLA RGLFTQDVQT NDRVKHRIVA RGYDKFFNIG EVPWTTWASL
ESHTGAPYIL TLKSNGCIIF IAALTPDKLL ITSKHALGPS NNAEYKSHAQ FGEQWLRKHL
LDAGKTEEQL AATLWEKNWT AVAELCDDSF EEHVLPYGPE KTGLHLHGLN ECTRQFKTMD
QPVVDAFAKE WGFIQTLSTV LDTVPQVREF TEAIGRTGKW NGEALEGFVV RTRVVDPPTK
GGQPAAASPY RAGSSFFFKV KFDEPYMMYR DWREVTKALL SKGPSMGNVP KSKLRRPETR
VYINWVIDEI KRDREQFATY THGKGIIATR ERFLNWLRSE EGSKAVKRQD ELPAERGLIR
EGEKFGKTII APIAVPGVGK TSIAVALSHL FGFGHIQSDD IKAKKAAPIF IKNVVNALKT
NDVVIADKNN HLIQHRQQLR EAVRTFKPPV RLMALNWSFD QPLSTIHRIC GDRVLQRGEN
HQSLHGDPLA KSHEDVIWQF MNQAEELADE EVDVAVEMDW EETLEDALTR AVDACVRILG
MPRPDQEKVG EALAIARGYA PSTKANKADS AKAKKQAVRY YAILAEVDLQ DVLGKRFAEA
GIPRDGKTFW ENLKAQKRIA PRPHITLVHQ KALPGETALW ERCKELHLHS NPPLFSFRLS
NLVWNDRVMA ATVSDFAIST DGSHQDAKGL DLVLKLPFEV QNRLHVTVGT RDGVPPVEAK
ALVESWKKNK HISGVGSLEL DNVWVKGSVK GLVN
//
