ID B9AH46_METSM Unreviewed; 474 AA.
AC B9AH46;
DT 24-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 24-MAR-2009, sequence version 1.
DT 27-MAR-2024, entry version 51.
DE SubName: Full=Radical SAM domain protein {ECO:0000313|EMBL:EEE42786.1};
GN ORFNames=METSMIALI_01705 {ECO:0000313|EMBL:EEE42786.1};
OS Methanobrevibacter smithii DSM 2375.
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanobrevibacter.
OX NCBI_TaxID=483214 {ECO:0000313|EMBL:EEE42786.1, ECO:0000313|Proteomes:UP000003489};
RN [1] {ECO:0000313|EMBL:EEE42786.1, ECO:0000313|Proteomes:UP000003489}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 2375 {ECO:0000313|EMBL:EEE42786.1,
RC ECO:0000313|Proteomes:UP000003489};
RA Fulton L., Clifton S., Fulton B., Xu J., Minx P., Pepin K.H., Johnson M.,
RA Bhonagiri V., Nash W.E., Mardis E.R., Wilson R.K.;
RL Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EEE42786.1, ECO:0000313|Proteomes:UP000003489}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 2375 {ECO:0000313|EMBL:EEE42786.1,
RC ECO:0000313|Proteomes:UP000003489};
RA Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D.,
RA Gordon J.;
RT "Draft genome sequence of Methanobrevibacter smithii (DSM 2375).";
RL Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEE42786.1}.
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DR EMBL; ABYW01000018; EEE42786.1; -; Genomic_DNA.
DR RefSeq; WP_004032128.1; NZ_DS996913.1.
DR AlphaFoldDB; B9AH46; -.
DR PATRIC; fig|483214.13.peg.1638; -.
DR HOGENOM; CLU_021572_4_3_2; -.
DR OrthoDB; 2305at2157; -.
DR Proteomes; UP000003489; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409:SF7; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE; 1.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485}.
FT DOMAIN 7..142
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 189..412
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 474 AA; 54015 MW; 8C3CD0BBB88B565C CRC64;
MKVLFVNPPQ TASKYKFMGV IAPPLGIAYM AGVLQENNID VEILDASAED MDFKDVEKEL
LKRKPDLVAL TALTPTIGRA LETAQVVKET LPDSIVVMGG YHPTFNFIET LEDENVDIVI
RGEGEYIMLN LVQALENQSS LHDVKGIVFE DKNSKEIVVN PEAPLIQDLD ELPFPALNLL
PMKKYRLLDM DTHMTTMITT RGCPMQCSFC SSAAMHGKKI RERSVENIVD EIEYLKTNYD
IDTIAFMDDT FTLKKRKVMA ICDEILKRNI EIMWGCTSRV DTLDEKLLKK MKEAGCITIF
IGVESADQQQ LDNMCKNTTI AKIENAFKIA HKLKIRTIAS VALGMPGDTK EIMNKTVKFV
HKLKPNYAIY SLATPYPGTR FYKEAFEKNL IKIKDWSKYT LITPILETID CSLNDMRKIQ
AKAFMKFYLR PHYIIRQFLQ DGPYLLKTIF GVIKTALSKT PKNTDYNKRE LKNM
//
