ID ILVD_CLOK1 Reviewed; 556 AA.
AC B9E299;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 01-MAY-2013, entry version 28.
DE RecName: Full=Dihydroxy-acid dehydratase;
DE Short=DAD;
DE EC=4.2.1.9;
GN Name=ilvD; OrderedLocusNames=CKR_1573;
OS Clostridium kluyveri (strain NBRC 12016).
OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=583346;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 12016;
RA Inui M., Nonaka H., Shinoda Y., Ikenaga Y., Abe M., Naito K.,
RA Vertes A.A., Yukawa H.;
RT "Complete genome sequence of Clostridium kluyveri and comparative
RT genomics of Clostridia species.";
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY: 2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-
CC oxobutanoate + H(2)O.
CC -!- COFACTOR: Binds 1 4Fe-4S cluster (Potential).
CC -!- PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-
CC isoleucine from 2-oxobutanoate: step 3/4.
CC -!- PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine
CC from pyruvate: step 3/4.
CC -!- SIMILARITY: Belongs to the IlvD/Edd family.
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DR EMBL; AP009049; BAH06624.1; -; Genomic_DNA.
DR RefSeq; YP_002472038.1; NC_011837.1.
DR STRING; 583346.CKR_1573; -.
DR EnsemblBacteria; BAH06624; BAH06624; CKR_1573.
DR GeneID; 7273173; -.
DR KEGG; ckr:CKR_1573; -.
DR PATRIC; 19473020; VBICloKlu118830_1757.
DR eggNOG; COG0129; -.
DR HOGENOM; HOG000173155; -.
DR KO; K01687; -.
DR ProtClustDB; PRK00911; -.
DR BioCyc; CKLU583346:GJNQ-1618-MONOMER; -.
DR UniPathway; UPA00047; UER00057.
DR UniPathway; UPA00049; UER00061.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0004160; F:dihydroxy-acid dehydratase activity; IEA:HAMAP.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009097; P:isoleucine biosynthetic process; IEA:HAMAP.
DR GO; GO:0009099; P:valine biosynthetic process; IEA:HAMAP.
DR HAMAP; MF_00012; IlvD; 1; -.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR004404; DihydroxyA_deHydtase.
DR InterPro; IPR000581; DiOHA_6PGluconate_deHydtase.
DR InterPro; IPR020558; DiOHA_6PGluconate_deHydtase_CS.
DR PANTHER; PTHR21000; PTHR21000; 1.
DR Pfam; PF00920; ILVD_EDD; 1.
DR SUPFAM; SSF52016; Aconitase/3IPM_dehydase_swvl; 1.
DR TIGRFAMs; TIGR00110; ilvD; 1.
DR PROSITE; PS00886; ILVD_EDD_1; 1.
DR PROSITE; PS00887; ILVD_EDD_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Amino-acid biosynthesis;
KW Branched-chain amino acid biosynthesis; Complete proteome; Iron;
KW Iron-sulfur; Lyase; Metal-binding.
FT CHAIN 1 556 Dihydroxy-acid dehydratase.
FT /FTId=PRO_1000190660.
FT METAL 119 119 Iron-sulfur (4Fe-4S) (Potential).
FT METAL 191 191 Iron-sulfur (4Fe-4S) (Potential).
SQ SEQUENCE 556 AA; 58826 MW; C7AF710A1BE73157 CRC64;
MRSDVITKGT KSAPQRALLN ALGLTKEEIE RPLVGIVSSK NDIVPGHMNL DKIVEAVKTG
VSMAGGTPIV FPAIAVCDGI AMGHQGMKYS LVTRDLIADS TEAMAMAHAF DALVMVPNCD
KNVPGLLMAA ARLNIPTIFV SGGPMLAGKV DGCKVSFSSI SEAVGAFNGG KITEEKLEEF
ESKVCPTCGS CSGMYTANSM NCLTEVLGMA LGGNGTIPAV YSDRIKLAKH AGMKIMELLE
RNIRPRDVMT EAAFKNALTM DMALGCSTNS MLHLPAIAHE AGIELNVDMA NEISAKTPNL
CHLAPAGHNY VEELNEAGGI YAVMNEINKL NLLSTDLITC TGKTVAENIK GCINKNKEVI
RPVENPYSTT GGIAILKGNL APDSCVVKRS AVAPEMLKHE GPARVFDCEE DALNAINTGK
IVAGDVVIIR YEGPKGGPGM REMLNPTSAI MGRGLGGSVA LITDGRFSGA TRGAAIGHVS
PEAAVGGNIA LVEEGDIIKI DIEANSIDFE ISEEELERRR SNWKPRQPKI TTGYLARYAS
MVTSGNRGAI LEIPKF
//
