ID DAPE_AGRRK Reviewed; 397 AA.
AC B9J8D0;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 01-MAY-2013, entry version 30.
DE RecName: Full=Succinyl-diaminopimelate desuccinylase;
DE Short=SDAP desuccinylase;
DE EC=3.5.1.18;
DE AltName: Full=N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase;
GN Name=dapE; OrderedLocusNames=Arad_0684;
OS Agrobacterium radiobacter (strain K84 / ATCC BAA-868).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=311403;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K84 / ATCC BAA-868;
RX PubMed=19251847; DOI=10.1128/JB.01779-08;
RA Slater S.C., Goldman B.S., Goodner B., Setubal J.C., Farrand S.K.,
RA Nester E.W., Burr T.J., Banta L., Dickerman A.W., Paulsen I.,
RA Otten L., Suen G., Welch R., Almeida N.F., Arnold F., Burton O.T.,
RA Du Z., Ewing A., Godsy E., Heisel S., Houmiel K.L., Jhaveri J., Lu J.,
RA Miller N.M., Norton S., Chen Q., Phoolcharoen W., Ohlin V.,
RA Ondrusek D., Pride N., Stricklin S.L., Sun J., Wheeler C., Wilson L.,
RA Zhu H., Wood D.W.;
RT "Genome sequences of three Agrobacterium biovars help elucidate the
RT evolution of multichromosome genomes in bacteria.";
RL J. Bacteriol. 191:2501-2511(2009).
CC -!- FUNCTION: Catalyzes the hydrolysis of N-succinyl-L,L-
CC diaminopimelic acid (SDAP), forming succinate and LL-2,6-
CC diaminoheptanedioate (DAP), an intermediate involved in the
CC bacterial biosynthesis of lysine and meso-diaminopimelic acid, an
CC essential component of bacterial cell walls (By similarity).
CC -!- CATALYTIC ACTIVITY: N-succinyl-LL-2,6-diaminoheptanedioate + H(2)O
CC = succinate + LL-2,6-diaminoheptanedioate.
CC -!- COFACTOR: Binds 1 Zn(2+) ion per subunit (By similarity).
CC -!- COFACTOR: Binds 1 Co(2+) ion per subunit (By similarity).
CC -!- PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP
CC pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate
CC (succinylase route): step 3/3.
CC -!- SUBUNIT: Homodimer (By similarity).
CC -!- SIMILARITY: Belongs to the peptidase M20A family. DapE subfamily.
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DR EMBL; CP000628; ACM25317.1; -; Genomic_DNA.
DR RefSeq; YP_002543242.1; NC_011985.1.
DR ProteinModelPortal; B9J8D0; -.
DR STRING; 311403.Arad_0684; -.
DR MEROPS; M20.010; -.
DR EnsemblBacteria; ACM25317; ACM25317; Arad_0684.
DR GeneID; 7371558; -.
DR KEGG; ara:Arad_0684; -.
DR PATRIC; 20801776; VBIAgrRad129173_2999.
DR eggNOG; COG0624; -.
DR HOGENOM; HOG000243770; -.
DR KO; K01439; -.
DR OMA; WDAPRLE; -.
DR ProtClustDB; PRK13009; -.
DR BioCyc; ARAD311403:GHU8-1103-MONOMER; -.
DR UniPathway; UPA00034; UER00021.
DR GO; GO:0050897; F:cobalt ion binding; IEA:HAMAP.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:InterPro.
DR GO; GO:0009014; F:succinyl-diaminopimelate desuccinylase activity; IEA:HAMAP.
DR GO; GO:0008270; F:zinc ion binding; IEA:HAMAP.
DR GO; GO:0019877; P:diaminopimelate biosynthetic process; IEA:HAMAP.
DR GO; GO:0009089; P:lysine biosynthetic process via diaminopimelate; IEA:HAMAP.
DR GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR HAMAP; MF_01690; DapE; 1; -.
DR InterPro; IPR001261; ArgE/DapE_CS.
DR InterPro; IPR005941; DapE_proteobac.
DR InterPro; IPR002933; Peptidase_M20.
DR InterPro; IPR011650; Peptidase_M20_dimer.
DR Pfam; PF07687; M20_dimer; 1.
DR Pfam; PF01546; Peptidase_M20; 1.
DR SUPFAM; SSF55031; Peptidase_M20_dimer; 1.
DR TIGRFAMs; TIGR01246; dapE_proteo; 1.
DR PROSITE; PS00758; ARGE_DAPE_CPG2_1; 1.
DR PROSITE; PS00759; ARGE_DAPE_CPG2_2; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Cobalt; Complete proteome;
KW Diaminopimelate biosynthesis; Hydrolase; Lysine biosynthesis;
KW Metal-binding; Zinc.
FT CHAIN 1 397 Succinyl-diaminopimelate desuccinylase.
FT /FTId=PRO_0000375455.
FT ACT_SITE 75 75 By similarity.
FT ACT_SITE 140 140 Proton acceptor (By similarity).
FT METAL 73 73 Cobalt or zinc 1 (By similarity).
FT METAL 106 106 Cobalt or zinc 1 (By similarity).
FT METAL 106 106 Cobalt or zinc 2 (By similarity).
FT METAL 141 141 Cobalt or zinc 2 (By similarity).
FT METAL 169 169 Cobalt or zinc 1 (By similarity).
FT METAL 366 366 Cobalt or zinc 2 (By similarity).
SQ SEQUENCE 397 AA; 42285 MW; A6C2A486F0457E03 CRC64;
MTATDPVANL QTLIRCASVT PAEGGALTAL ADMLLPLGFK VERMTASEAG TPDIENLYAR
LGTEGPHLMF AGHTDVVPVG DAASWSHPPF AADIAGGELF GRGAVDMKGG IACFAAAVAR
HIEKHGPPAG SISFLITGDE EGPAINGTVK LLQWAAERGE QWDASLVGEP TNPDQLGDMI
KIGRRGSISG FITVHGVQGH AAYPHLADNP VRSIVKLTEA LLDPPFDAGT DNFQPSNLEV
TTIDVGNAAT NVIPAKATAA FNIRFNDTWT VETLRAEILA RLDAAAADQT LRPGREPTKY
DITWSDRPSQ VFLTRNNALI ASLSSAVENV TGHTPKLSTT GGTSDARFIK DYCPVVEFGL
VGQTMHMVDE RVAVADLETL TEIYETFIQR WFANAEL
//
