ID RNC_AGRRK Reviewed; 239 AA.
AC B9JC73;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 01-MAY-2013, entry version 32.
DE RecName: Full=Ribonuclease 3;
DE EC=3.1.26.3;
DE AltName: Full=Ribonuclease III;
DE Short=RNase III;
GN Name=rnc; OrderedLocusNames=Arad_1593;
OS Agrobacterium radiobacter (strain K84 / ATCC BAA-868).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=311403;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K84 / ATCC BAA-868;
RX PubMed=19251847; DOI=10.1128/JB.01779-08;
RA Slater S.C., Goldman B.S., Goodner B., Setubal J.C., Farrand S.K.,
RA Nester E.W., Burr T.J., Banta L., Dickerman A.W., Paulsen I.,
RA Otten L., Suen G., Welch R., Almeida N.F., Arnold F., Burton O.T.,
RA Du Z., Ewing A., Godsy E., Heisel S., Houmiel K.L., Jhaveri J., Lu J.,
RA Miller N.M., Norton S., Chen Q., Phoolcharoen W., Ohlin V.,
RA Ondrusek D., Pride N., Stricklin S.L., Sun J., Wheeler C., Wilson L.,
RA Zhu H., Wood D.W.;
RT "Genome sequences of three Agrobacterium biovars help elucidate the
RT evolution of multichromosome genomes in bacteria.";
RL J. Bacteriol. 191:2501-2511(2009).
CC -!- FUNCTION: Digests double-stranded RNA. Involved in the processing
CC of primary rRNA transcript to yield the immediate precursors to
CC the large and small rRNAs (23S and 16S). Also processes some
CC mRNAs, and tRNAs when they are encoded in the rRNA operon (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
CC phosphomonoester.
CC -!- COFACTOR: Mg(2+) (By similarity).
CC -!- SUBUNIT: Homodimer (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Contains 1 DRBM (double-stranded RNA-binding) domain.
CC -!- SIMILARITY: Contains 1 RNase III domain.
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DR EMBL; CP000628; ACM25994.1; -; Genomic_DNA.
DR RefSeq; YP_002543920.1; NC_011985.1.
DR STRING; 311403.Arad_1593; -.
DR EnsemblBacteria; ACM25994; ACM25994; Arad_1593.
DR GeneID; 7368306; -.
DR KEGG; ara:Arad_1593; -.
DR PATRIC; 20803210; VBIAgrRad129173_3709.
DR eggNOG; COG0571; -.
DR HOGENOM; HOG000246809; -.
DR KO; K03685; -.
DR OMA; IDNVNDQ; -.
DR ProtClustDB; PRK12371; -.
DR BioCyc; ARAD311403:GHU8-1786-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004525; F:ribonuclease III activity; IEA:HAMAP.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006397; P:mRNA processing; IEA:HAMAP.
DR GO; GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
DR GO; GO:0016075; P:rRNA catabolic process; IEA:InterPro.
DR GO; GO:0006364; P:rRNA processing; IEA:HAMAP.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1520.10; -; 1.
DR Gene3D; 3.30.160.20; -; 1.
DR HAMAP; MF_00104; RNase_III; 1; -.
DR InterPro; IPR001159; Ds-RNA-bd.
DR InterPro; IPR014720; dsRNA-bd-like_dom.
DR InterPro; IPR011907; RNase_III.
DR InterPro; IPR000999; RNase_III_dom.
DR PANTHER; PTHR11207; PTHR11207; 1.
DR Pfam; PF00035; dsrm; 1.
DR Pfam; PF00636; Ribonuclease_3; 1.
DR SMART; SM00358; DSRM; 1.
DR SMART; SM00535; RIBOc; 1.
DR SUPFAM; SSF69065; RNase_III; 1.
DR TIGRFAMs; TIGR02191; RNaseIII; 1.
DR PROSITE; PS50137; DS_RBD; 1.
DR PROSITE; PS00517; RNASE_3_1; 1.
DR PROSITE; PS50142; RNASE_3_2; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Endonuclease; Hydrolase; Magnesium;
KW Metal-binding; mRNA processing; Nuclease; RNA-binding;
KW rRNA processing; rRNA-binding; tRNA processing.
FT CHAIN 1 239 Ribonuclease 3.
FT /FTId=PRO_1000118908.
FT DOMAIN 12 137 RNase III.
FT DOMAIN 162 231 DRBM.
FT ACT_SITE 54 54 Potential.
FT ACT_SITE 126 126 By similarity.
FT METAL 50 50 Magnesium (By similarity).
FT METAL 123 123 Magnesium (By similarity).
FT METAL 126 126 Magnesium (By similarity).
SQ SEQUENCE 239 AA; 26552 MW; FD0646CDBD53AE3E CRC64;
MTKTPTLSQA DRLTLEAAIG HAFVEKERLD WALTHASART HKAGNYERLE FLGDRVLGLC
IAELLFRTFG TATEGELSVR LNQLVSAETC AAVADEMQLH LFIRTGADVK KLTGKRMLNV
RADVVESLIA ALYLDGGLEV ARKFILRYWE GRAVRPDGAR RDAKTELQEW AHAKFGVTPV
YRVDDRSGPD HDPRFTVTVE VAGAAPETGI ERSKRAAEQV AATRILEREG IWQPQSAQK
//
