ID B9LSW4_HALLT Unreviewed; 424 AA.
AC B9LSW4;
DT 24-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 24-MAR-2009, sequence version 1.
DT 27-MAR-2024, entry version 55.
DE SubName: Full=Transposase, IS605 OrfB family {ECO:0000313|EMBL:ACM56029.1};
GN OrderedLocusNames=Hlac_0426 {ECO:0000313|EMBL:ACM56029.1}, Hlac_0436
GN {ECO:0000313|EMBL:ACM56038.1};
OS Halorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM
OS 34).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Halorubraceae; Halorubrum.
OX NCBI_TaxID=416348 {ECO:0000313|EMBL:ACM56029.1, ECO:0000313|Proteomes:UP000000740};
RN [1] {ECO:0000313|EMBL:ACM56029.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 49239 {ECO:0000313|EMBL:ACM56029.1};
RX PubMed=23320757; DOI=10.1186/1472-6750-13-3;
RA Karan R., Capes M.D., DasSarma P., DasSarma S.;
RT "Cloning, overexpression, purification, and characterization of a
RT polyextremophilic beta-galactosidase from the Antarctic haloarchaeon
RT Halorubrum lacusprofundi.";
RL BMC Biotechnol. 13:3-3(2013).
RN [2] {ECO:0000313|EMBL:ACM56029.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 49239 {ECO:0000313|EMBL:ACM56029.1};
RX PubMed=23536799;
RA Dassarma S., Capes M.D., Karan R., Dassarma P.;
RT "Amino acid substitutions in cold-adapted proteins from Halorubrum
RT lacusprofundi, an extremely halophilic microbe from antarctica.";
RL PLoS ONE 8:e58587-e58587(2013).
RN [3] {ECO:0000313|EMBL:ACM56029.1, ECO:0000313|Proteomes:UP000000740}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49239 {ECO:0000313|EMBL:ACM56029.1}, and ATCC 49239 / DSM
RC 5036 / JCM 8891 / ACAM 34 {ECO:0000313|Proteomes:UP000000740};
RX PubMed=27617060; DOI=10.1186/s40793-016-0194-2;
RA Anderson I.J., DasSarma P., Lucas S., Copeland A., Lapidus A.,
RA Del Rio T.G., Tice H., Dalin E., Bruce D.C., Goodwin L., Pitluck S.,
RA Sims D., Brettin T.S., Detter J.C., Han C.S., Larimer F., Hauser L.,
RA Land M., Ivanova N., Richardson P., Cavicchioli R., DasSarma S.,
RA Woese C.R., Kyrpides N.C.;
RT "Complete genome sequence of the Antarctic Halorubrum lacusprofundi type
RT strain ACAM 34.";
RL Stand. Genomic Sci. 11:70-70(2016).
RN [4] {ECO:0000313|EMBL:ACM56029.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 49239 {ECO:0000313|EMBL:ACM56029.1};
RX PubMed=29109294;
RA Laye V.J., Karan R., Kim J.M., Pecher W.T., DasSarma P., DasSarma S.;
RT "Key amino acid residues conferring enhanced enzyme activity at cold
RT temperatures in an Antarctic polyextremophilic beta-galactosidase.";
RL Proc. Natl. Acad. Sci. U.S.A. 114:12530-12535(2017).
RN [5] {ECO:0000313|EMBL:ACM56029.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 49239 {ECO:0000313|EMBL:ACM56029.1};
RX PubMed=29189043;
RA Laye V.J., DasSarma S.;
RT "An Antarctic Extreme Halophile and Its Polyextremophilic Enzyme: Effects
RT of Perchlorate Salts.";
RL Astrobiology 18:412-418(2018).
RN [6] {ECO:0000313|EMBL:ACM56029.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 49239 {ECO:0000313|EMBL:ACM56029.1};
RX PubMed=31463573;
RA DasSarma S., DasSarma P., Laye V.J., Schwieterman E.W.;
RT "Extremophilic models for astrobiology: haloarchaeal survival strategies
RT and pigments for remote sensing.";
RL Extremophiles 24:31-41(2020).
CC -!- SIMILARITY: In the C-terminal section; belongs to the transposase 35
CC family. {ECO:0000256|ARBA:ARBA00008761}.
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DR EMBL; CP001365; ACM56029.1; -; Genomic_DNA.
DR EMBL; CP001365; ACM56038.1; -; Genomic_DNA.
DR RefSeq; WP_012659667.1; NC_012029.1.
DR GeneID; 7401054; -.
DR KEGG; hla:Hlac_0426; -.
DR KEGG; hla:Hlac_0436; -.
DR eggNOG; arCOG00683; Archaea.
DR HOGENOM; CLU_040997_0_0_2; -.
DR Proteomes; UP000000740; Chromosome 1.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0032196; P:transposition; IEA:UniProtKB-KW.
DR InterPro; IPR010095; Cas12f1-like_TNB.
DR InterPro; IPR001959; Transposase.
DR NCBIfam; NF040570; guided_TnpB; 1.
DR Pfam; PF01385; OrfB_IS605; 1.
DR Pfam; PF07282; OrfB_Zn_ribbon; 1.
PE 3: Inferred from homology;
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Reference proteome {ECO:0000313|Proteomes:UP000000740};
KW Transposition {ECO:0000256|ARBA:ARBA00022578}.
FT DOMAIN 178..292
FT /note="Probable transposase IS891/IS1136/IS1341"
FT /evidence="ECO:0000259|Pfam:PF01385"
FT DOMAIN 311..377
FT /note="Cas12f1-like TNB"
FT /evidence="ECO:0000259|Pfam:PF07282"
SQ SEQUENCE 424 AA; 49276 MW; B85DAC38B2A8256D CRC64;
MKRANTFEVV PQTENDKECL LRLLDASASL WNELTYERRQ NYFGDGDVWD TSEYRGRYNG
VVGSATVQQV TRKNSEAWRS FFALKEKGEY ANPPSYWGNE EDGRELRTYI RCNQYTIEWG
KRSRLEIPVG QELKDEYGLG YHERLRLEVR GNPKWDGKQG RLELEYDEVS DTFRAFQPVT
VPDSRLDSPL ASEEAALDVG ANNLVACSTT TGNQYLYDGR ELFGRFRETT DEIARLQSKL
REGRYSSNRI RRLYRQRTKR RDHAQNALVR DLVERLYDEG VATVYVGDLT DVLEAHWSVR
VNEKTHNFWA FKKFIHRLAC VCEEYGISLE TESEAWTSQT CPECGDHEKT VRHEDTLTCP
CGFEGHADLT ASETFLRENS NCEIRPMARP VRFEWDDHDW SGKLYPHESP KEVRTNPQVA
SVGR
//
