ID B9T9B5_RICCO Unreviewed; 215 AA.
AC B9T9B5;
DT 24-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 24-MAR-2009, sequence version 1.
DT 24-JAN-2024, entry version 42.
DE RecName: Full=nitrile hydratase {ECO:0000256|ARBA:ARBA00013079};
DE EC=4.2.1.84 {ECO:0000256|ARBA:ARBA00013079};
GN ORFNames=RCOM_0141170 {ECO:0000313|EMBL:EEF27548.1};
OS Ricinus communis (Castor bean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Malpighiales; Euphorbiaceae; Acalyphoideae; Acalypheae;
OC Ricinus.
OX NCBI_TaxID=3988 {ECO:0000313|Proteomes:UP000008311};
RN [1] {ECO:0000313|Proteomes:UP000008311}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Hale {ECO:0000313|Proteomes:UP000008311};
RX PubMed=20729833; DOI=10.1038/nbt.1674;
RA Chan A.P., Crabtree J., Zhao Q., Lorenzi H., Orvis J., Puiu D.,
RA Melake-Berhan A., Jones K.M., Redman J., Chen G., Cahoon E.B., Gedil M.,
RA Stanke M., Haas B.J., Wortman J.R., Fraser-Liggett C.M., Ravel J.,
RA Rabinowicz P.D.;
RT "Draft genome sequence of the oilseed species Ricinus communis.";
RL Nat. Biotechnol. 28:951-956(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an aliphatic amide = a nitrile + H2O; Xref=Rhea:RHEA:12673,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:18379, ChEBI:CHEBI:65285; EC=4.2.1.84;
CC Evidence={ECO:0000256|ARBA:ARBA00001410};
CC -!- SIMILARITY: Belongs to the nitrile hydratase subunit alpha family.
CC {ECO:0000256|ARBA:ARBA00009363}.
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DR EMBL; EQ975337; EEF27548.1; -; Genomic_DNA.
DR AlphaFoldDB; B9T9B5; -.
DR STRING; 3988.B9T9B5; -.
DR eggNOG; ENOG502S164; Eukaryota.
DR InParanoid; B9T9B5; -.
DR Proteomes; UP000008311; Unassembled WGS sequence.
DR GO; GO:0080109; F:indole-3-acetonitrile nitrile hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046914; F:transition metal ion binding; IEA:InterPro.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR Gene3D; 3.90.330.10; Nitrile hydratase alpha /Thiocyanate hydrolase gamma; 1.
DR InterPro; IPR036648; CN_Hdrase_a/SCN_Hdrase_g_sf.
DR InterPro; IPR004232; CN_Hdrtase_a/SCN_Hdrlase_g.
DR InterPro; IPR023900; CN_Hdrtase_asu/SCN_Hdrlase_gsu.
DR InterPro; IPR018141; Nitrile_hydratase_asu.
DR NCBIfam; TIGR01323; nitrile_alph; 1.
DR Pfam; PF02979; NHase_alpha; 1.
DR PIRSF; PIRSF001426; NHase_alpha; 1.
DR SUPFAM; SSF56209; Nitrile hydratase alpha chain; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|EMBL:EEF27548.1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000008311}.
FT DOMAIN 25..205
FT /note="Nitrile hydratase alpha /Thiocyanate hydrolase
FT gamma"
FT /evidence="ECO:0000259|Pfam:PF02979"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 215 AA; 23730 MW; C08D2B360D0A18A6 CRC64;
MSPVSNKERS MQSPDEKTQP PSPPTLRIKA IQSLLIEKGV LDPAAVDEII DIFEHRLGPK
NGASIIAKAW TDPAFKQYLL DDATAAIHGM GFTGMEGAHL YVAENTPEVH NLVVCTLCSC
YPWPVLGLPP TWFKAPQYRS RAVIDPRSVL EEFGLQVPDE KEIRVWDSNA EIRYLVLPER
PQGSEGMTEQ QLAQLVTRDS MIGTALLAPV EGEIA
//
