ID B9XFS1_PEDPL Unreviewed; 730 AA.
AC B9XFS1;
DT 14-APR-2009, integrated into UniProtKB/TrEMBL.
DT 14-APR-2009, sequence version 1.
DT 24-JAN-2024, entry version 46.
DE SubName: Full=Alpha-N-arabinofuranosidase {ECO:0000313|EMBL:EEF61435.1};
DE EC=3.2.1.55 {ECO:0000313|EMBL:EEF61435.1};
DE Flags: Precursor;
GN ORFNames=Cflav_PD4456 {ECO:0000313|EMBL:EEF61435.1};
OS Pedosphaera parvula (strain Ellin514).
OC Bacteria; Verrucomicrobiota; Verrucomicrobiae; Limisphaerales; Pedosphaera.
OX NCBI_TaxID=320771 {ECO:0000313|EMBL:EEF61435.1, ECO:0000313|Proteomes:UP000003688};
RN [1] {ECO:0000313|EMBL:EEF61435.1, ECO:0000313|Proteomes:UP000003688}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ellin514 {ECO:0000313|EMBL:EEF61435.1,
RC ECO:0000313|Proteomes:UP000003688};
RX PubMed=21460084; DOI=10.1128/JB.00299-11;
RA Kant R., van Passel M.W., Sangwan P., Palva A., Lucas S., Copeland A.,
RA Lapidus A., Glavina Del Rio T., Dalin E., Tice H., Bruce D., Goodwin L.,
RA Pitluck S., Chertkov O., Larimer F.W., Land M.L., Hauser L., Brettin T.S.,
RA Detter J.C., Han S., de Vos W.M., Janssen P.H., Smidt H.;
RT "Genome sequence of 'Pedosphaera parvula' Ellin514, an aerobic
RT Verrucomicrobial isolate from pasture soil.";
RL J. Bacteriol. 193:2900-2901(2011).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEF61435.1}.
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DR EMBL; ABOX02000010; EEF61435.1; -; Genomic_DNA.
DR RefSeq; WP_007414667.1; NZ_ABOX02000010.1.
DR AlphaFoldDB; B9XFS1; -.
DR STRING; 320771.Cflav_PD4456; -.
DR OrthoDB; 177947at2; -.
DR Proteomes; UP000003688; Unassembled WGS sequence.
DR GO; GO:0046556; F:alpha-L-arabinofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18820; GH43_LbAraf43-like; 1.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR43817; GLYCOSYL HYDROLASE; 1.
DR PANTHER; PTHR43817:SF1; HYDROLASE, FAMILY 43, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01660)-RELATED; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF89372; Fucose-specific lectin; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000313|EMBL:EEF61435.1};
KW Hydrolase {ECO:0000313|EMBL:EEF61435.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000003688};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..730
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002894760"
FT ACT_SITE 32
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 204
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 141
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 730 AA; 78071 MW; CA409EF29C591AC0 CRC64;
MNFKYLLVLF CWLAGSVFGA PFFNPIVSNG ADPWVVAKDG FYYYTQTTGA SVKVRQTRRI
AGAIGISNTT ATTVFTPSAP YNQNVWAPEL HYLQGKWYIY YAADDGTDAN HRLYVAEASS
PLGPYTLKGK IADPSDCWAI DGTVLEKDDG TLYLVWSGWT GATHGNQNIY IAPMSNPWTI
SGPRVNLSTI AYSWEYPNST FVNEGPEILK RNGKTCIVYS ANGTWTDNYC LGLMVNTDGN
YLNAASWTKN SSPIFKTYVG ADGAVYAPGH CGFTHSLDGT EDWIIYHAAQ YQGAGFTRDV
RAQKFTWLAN DTPNLGQPIP PGVPVASPSG EERALESATL LSDGRLAVCT VPTNGVVQRN
FRLPGTGSWS GWGSLGGSGF KKISTVRYND DRLAVLGIGS DARVWLNAEM SSGGAWMGWA
YLGGPAFSEV KGVIYSDQRL AGAAVGDGTA MWLNSQSSTN GSWGGWVSLG GSNFWNVDLV
KRLDDRLVVL GVGSTDVWIN AQATVNGSWN GWQSIGGTNF NYARMLSRPD GRLAVFAVGS
GTAAWINSEL TPEGAWSGWT SLAGSVLTRL EPILLSDGRM AVFATGDYSA VTVNQQATVN
GAWSGWVTVA GSGFGSTTGV QMPDGQLAAL THNFGTAEYF NQQSGPSHIW GGWSALVGGA
EAPKLSCSQA GDSVTLQWST NVSGFKLEQS VQTVPSSWGA LTNVPTVVGT NNSVTLTIDP
AGKVFRMRHP
//
