ID BAMA_NEIMB Reviewed; 797 AA.
AC Q9K1H0;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 27-MAR-2024, entry version 113.
DE RecName: Full=Outer membrane protein assembly factor BamA {ECO:0000255|HAMAP-Rule:MF_01430};
DE AltName: Full=Outer membrane protein Omp85;
DE Flags: Precursor;
GN Name=bamA {ECO:0000255|HAMAP-Rule:MF_01430}; Synonyms=omp85;
GN OrderedLocusNames=NMB0182;
OS Neisseria meningitidis serogroup B (strain MC58).
OC Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC Neisseria.
OX NCBI_TaxID=122586;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MC58;
RX PubMed=10710307; DOI=10.1126/science.287.5459.1809;
RA Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E.,
RA Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C.,
RA Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H.,
RA Salzberg S.L., White O., Fleischmann R.D., Dougherty B.A., Mason T.M.,
RA Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D.,
RA Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V.,
RA Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M.,
RA Moxon E.R., Rappuoli R., Venter J.C.;
RT "Complete genome sequence of Neisseria meningitidis serogroup B strain
RT MC58.";
RL Science 287:1809-1815(2000).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=17038831; DOI=10.4161/hv.1.2.1651;
RA Vipond C., Wheeler J.X., Jones C., Feavers I.M., Suker J.;
RT "Characterization of the protein content of a meningococcal outer membrane
RT vesicle vaccine by polyacrylamide gel electrophoresis and mass
RT spectrometry.";
RL Hum. Vaccin. 1:80-84(2005).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=NZ98/254 / Serogroup B;
RX PubMed=16645985; DOI=10.1002/pmic.200500821;
RA Vipond C., Suker J., Jones C., Tang C., Feavers I.M., Wheeler J.X.;
RT "Proteomic analysis of a meningococcal outer membrane vesicle vaccine
RT prepared from the group B strain NZ98/254.";
RL Proteomics 6:3400-3413(2006).
CC -!- FUNCTION: Part of the outer membrane protein assembly complex, which is
CC involved in assembly and insertion of beta-barrel proteins into the
CC outer membrane. {ECO:0000255|HAMAP-Rule:MF_01430}.
CC -!- SUBUNIT: Part of the Bam complex. {ECO:0000255|HAMAP-Rule:MF_01430}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000255|HAMAP-
CC Rule:MF_01430}.
CC -!- MISCELLANEOUS: Present in outer membrane vesicle formulations which are
CC used as vaccines in human.
CC -!- SIMILARITY: Belongs to the BamA family. {ECO:0000255|HAMAP-
CC Rule:MF_01430}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE002098; AAF40639.1; -; Genomic_DNA.
DR PIR; G81228; G81228.
DR RefSeq; NP_273240.1; NC_003112.2.
DR RefSeq; WP_002243947.1; NC_003112.2.
DR AlphaFoldDB; Q9K1H0; -.
DR SMR; Q9K1H0; -.
DR STRING; 122586.NMB0182; -.
DR TCDB; 1.B.33.1.1; the outer membrane protein insertion porin (bam complex) (ompip) family.
DR PaxDb; 122586-NMB0182; -.
DR KEGG; nme:NMB0182; -.
DR PATRIC; fig|122586.8.peg.224; -.
DR HOGENOM; CLU_007664_1_0_4; -.
DR InParanoid; Q9K1H0; -.
DR OrthoDB; 9803054at2; -.
DR Proteomes; UP000000425; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043165; P:Gram-negative-bacterium-type cell outer membrane assembly; IEA:UniProtKB-UniRule.
DR GO; GO:0051205; P:protein insertion into membrane; IEA:UniProtKB-UniRule.
DR Gene3D; 3.10.20.310; membrane protein fhac; 5.
DR Gene3D; 2.40.160.50; membrane protein fhac: a member of the omp85/tpsb transporter family; 1.
DR HAMAP; MF_01430; OM_assembly_BamA; 1.
DR InterPro; IPR000184; Bac_surfAg_D15.
DR InterPro; IPR010827; BamA/TamA_POTRA.
DR InterPro; IPR039910; D15-like.
DR InterPro; IPR023707; OM_assembly_BamA.
DR InterPro; IPR034746; POTRA.
DR NCBIfam; TIGR03303; OM_YaeT; 1.
DR PANTHER; PTHR12815:SF23; OUTER MEMBRANE PROTEIN ASSEMBLY FACTOR BAMA; 1.
DR PANTHER; PTHR12815; SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER; 1.
DR Pfam; PF01103; Omp85; 1.
DR Pfam; PF07244; POTRA; 5.
DR PIRSF; PIRSF006076; OM_assembly_OMP85; 1.
DR PROSITE; PS51779; POTRA; 5.
PE 1: Evidence at protein level;
KW Cell outer membrane; Membrane; Reference proteome; Repeat; Signal;
KW Transmembrane; Transmembrane beta strand.
FT SIGNAL 1..21
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01430"
FT CHAIN 22..797
FT /note="Outer membrane protein assembly factor BamA"
FT /id="PRO_0000349887"
FT DOMAIN 23..90
FT /note="POTRA 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01115"
FT DOMAIN 91..171
FT /note="POTRA 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01115"
FT DOMAIN 174..262
FT /note="POTRA 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01115"
FT DOMAIN 265..344
FT /note="POTRA 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01115"
FT DOMAIN 347..421
FT /note="POTRA 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01115"
SQ SEQUENCE 797 AA; 88437 MW; B68BFC5A43D22EE8 CRC64;
MKLKQIASAL MMLGISPLAL ADFTIQDIRV EGLQRTEPST VFNYLPVKVG DTYNDTHGSA
IIKSLYATGF FDDVRVETAD GQLLLTVIER PTIGSLNITG AKMLQNDAIK KNLESFGLAQ
SQYFNQATLN QAVAGLKEEY LGRGKLNIQI TPKVTKLARN RVDIDITIDE GKSAKITDIE
FEGNQVYSDR KLMRQMSLTE GGIWTWLTRS NQFNEQKFAQ DMEKVTDFYQ NNGYFDFRIL
DTDIQTNEDK TKQTIKITVH EGGRFRWGKV SIEGDTNEVP KAELEKLLTM KPGKWYERQQ
MTAVLGEIQN RMGSAGYAYS EISVQPLPNA ETKTVDFVLH IEPGRKIYVN EIHITGNNKT
RDEVVRRELR QMESAPYDTS KLQRSKERVE LLGYFDNVQF DAVPLAGTPD KVDLNMSLTE
RSTGSLDLSA GWVQDTGLVM SAGVSQDNLF GTGKSAALRA SRSKTTLNGS LSFTDPYFTA
DGVSLGYDVY GKAFDPRKAS TSIKQYKTTT AGAGIRMSVP VTEYDRVNFG LVAEHLTVNT
YNKAPKHYAD FIKKYGKTDG TDGSFKGWLY KGTVGWGRNK TDSALWPTRG YLTGVNAEIA
LPGSKLQYYS ATHNQTWFFP LSKTFTLMLG GEVGIAGGYG RTKEIPFFEN FYGGGLGSVR
GYESGTLGPK VYDEYGEKIS YGGNKKANVS AELLFPMPGA KDARTVRLSL FADAGSVWDG
KTYDDNSSSA TGGRVQNIYG AGNTHKSTFT NELRYSAGGA VTWLSPLGPM KFSYAYPLKK
KPEDEIQRFQ FQLGTTF
//
