ID C1ASL2_RHOOB Unreviewed; 480 AA.
AC C1ASL2;
DT 26-MAY-2009, integrated into UniProtKB/TrEMBL.
DT 26-MAY-2009, sequence version 1.
DT 27-MAR-2024, entry version 77.
DE RecName: Full=alpha,alpha-trehalose-phosphate synthase (ADP-forming) {ECO:0000256|ARBA:ARBA00012842};
DE EC=2.4.1.347 {ECO:0000256|ARBA:ARBA00012842};
GN Name=otsA {ECO:0000313|EMBL:BAH48461.1};
GN OrderedLocusNames=ROP_02140 {ECO:0000313|EMBL:BAH48461.1};
OS Rhodococcus opacus (strain B4).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC Rhodococcus.
OX NCBI_TaxID=632772 {ECO:0000313|EMBL:BAH48461.1, ECO:0000313|Proteomes:UP000002212};
RN [1] {ECO:0000313|EMBL:BAH48461.1, ECO:0000313|Proteomes:UP000002212}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B4 {ECO:0000313|EMBL:BAH48461.1,
RC ECO:0000313|Proteomes:UP000002212};
RA Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S.,
RA Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.;
RT "Comparison of the complete genome sequences of Rhodococcus erythropolis
RT PR4 and Rhodococcus opacus B4.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ADP-alpha-D-glucose + D-glucose 6-phosphate = ADP +
CC alpha,alpha-trehalose 6-phosphate + H(+); Xref=Rhea:RHEA:53880,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57498, ChEBI:CHEBI:58429,
CC ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.4.1.347;
CC Evidence={ECO:0000256|ARBA:ARBA00001801};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=CDP-alpha-D-glucose + D-glucose 6-phosphate = alpha,alpha-
CC trehalose 6-phosphate + CDP + H(+); Xref=Rhea:RHEA:53884,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58069, ChEBI:CHEBI:58429,
CC ChEBI:CHEBI:61548, ChEBI:CHEBI:137927;
CC Evidence={ECO:0000256|ARBA:ARBA00001525};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glucose 6-phosphate + GDP-alpha-D-glucose = alpha,alpha-
CC trehalose 6-phosphate + GDP + H(+); Xref=Rhea:RHEA:14605,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58189, ChEBI:CHEBI:58429,
CC ChEBI:CHEBI:61548, ChEBI:CHEBI:62230;
CC Evidence={ECO:0000256|ARBA:ARBA00000839};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glucose 6-phosphate + TDP-alpha-D-glucose = alpha,alpha-
CC trehalose 6-phosphate + H(+) + TDP; Xref=Rhea:RHEA:53888,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58429, ChEBI:CHEBI:61417,
CC ChEBI:CHEBI:61548, ChEBI:CHEBI:137931;
CC Evidence={ECO:0000256|ARBA:ARBA00000159};
CC -!- SIMILARITY: Belongs to the glycosyltransferase 20 family.
CC {ECO:0000256|ARBA:ARBA00008799}.
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DR EMBL; AP011115; BAH48461.1; -; Genomic_DNA.
DR RefSeq; WP_012687470.1; NC_012522.1.
DR AlphaFoldDB; C1ASL2; -.
DR STRING; 632772.ROP_02140; -.
DR CAZy; GT20; Glycosyltransferase Family 20.
DR KEGG; rop:ROP_02140; -.
DR PATRIC; fig|632772.20.peg.247; -.
DR HOGENOM; CLU_002351_7_1_11; -.
DR OrthoDB; 9761633at2; -.
DR Proteomes; UP000002212; Chromosome.
DR GO; GO:0047260; F:alpha,alpha-trehalose-phosphate synthase (GDP-forming) activity; IEA:RHEA.
DR GO; GO:0005992; P:trehalose biosynthetic process; IEA:InterPro.
DR CDD; cd03788; GT20_TPS; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 2.
DR InterPro; IPR001830; Glyco_trans_20.
DR PANTHER; PTHR10788:SF106; BCDNA.GH08860; 1.
DR PANTHER; PTHR10788; TREHALOSE-6-PHOSPHATE SYNTHASE; 1.
DR Pfam; PF00982; Glyco_transf_20; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000313|EMBL:BAH48461.1};
KW Transferase {ECO:0000313|EMBL:BAH48461.1}.
SQ SEQUENCE 480 AA; 53307 MW; 54147F8E3F6DA61C CRC64;
MNDSAPHGTS DFVVVANRLP VDMETLDDGT TRWKRSPGGL VTALEPILLG RERGSWVGWP
GVPDTKVEPI VSGGVDLFPV ELSSDEVARY YEGFSNATLW PLYHDVLVAP VFDRDWWHTY
LEVNRHFAEE TARTAAEGAT VWVQDYQLQL VPRLLREMRP DLTIGFFLHI PFPPVELFMQ
LPWRKEIVEG LLGADLIGFH LPGGAHNFLF LARRLLGLDA TTAGVGVRGA LGTVTLDDRV
VHVGAFPISV DARKLVEQAT EPAVVERAAE IRRELGNPRK ILLGVDRLDY TKGIDLRLSA
LGELLDEGRL DPSDTVMVQL ATPSRERVEH YMKMRSSIEE QVSHINGDHG QIGRPVVHYL
YQPVDRSELI ALFLAADVML VTPMRDGMNL VAKEYVACRA DLGGALVLSE FAGAAAELRG
AYLVNPHDLD GVKNTIVAAL GQEREQGRRR MSRLHRQVLD HDVHRWAQSF LGALDPNAAD
//
