ID C1EFK8_MICCC Unreviewed; 294 AA.
AC C1EFK8;
DT 26-MAY-2009, integrated into UniProtKB/TrEMBL.
DT 26-MAY-2009, sequence version 1.
DT 27-MAR-2024, entry version 61.
DE RecName: Full=Pseudouridine synthase I TruA alpha/beta domain-containing protein {ECO:0000259|Pfam:PF01416};
DE Flags: Fragment;
GN ORFNames=MICPUN_69585 {ECO:0000313|EMBL:ACO66872.1};
OS Micromonas commoda (strain RCC299 / NOUM17 / CCMP2709) (Picoplanktonic
OS green alga).
OC Eukaryota; Viridiplantae; Chlorophyta; Mamiellophyceae; Mamiellales;
OC Mamiellaceae; Micromonas.
OX NCBI_TaxID=296587 {ECO:0000313|EMBL:ACO66872.1, ECO:0000313|Proteomes:UP000002009};
RN [1] {ECO:0000313|EMBL:ACO66872.1, ECO:0000313|Proteomes:UP000002009}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RCC299 / NOUM17 {ECO:0000313|Proteomes:UP000002009};
RX PubMed=19359590; DOI=10.1126/science.1167222;
RA Worden A.Z., Lee J.H., Mock T., Rouze P., Simmons M.P., Aerts A.L.,
RA Allen A.E., Cuvelier M.L., Derelle E., Everett M.V., Foulon E.,
RA Grimwood J., Gundlach H., Henrissat B., Napoli C., McDonald S.M.,
RA Parker M.S., Rombauts S., Salamov A., Von Dassow P., Badger J.H.,
RA Coutinho P.M., Demir E., Dubchak I., Gentemann C., Eikrem W., Gready J.E.,
RA John U., Lanier W., Lindquist E.A., Lucas S., Mayer K.F., Moreau H.,
RA Not F., Otillar R., Panaud O., Pangilinan J., Paulsen I., Piegu B.,
RA Poliakov A., Robbens S., Schmutz J., Toulza E., Wyss T., Zelensky A.,
RA Zhou K., Armbrust E.V., Bhattacharya D., Goodenough U.W., Van de Peer Y.,
RA Grigoriev I.V.;
RT "Green evolution and dynamic adaptations revealed by genomes of the marine
RT picoeukaryotes Micromonas.";
RL Science 324:268-272(2009).
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000256|ARBA:ARBA00009375}.
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DR EMBL; CP001331; ACO66872.1; -; Genomic_DNA.
DR RefSeq; XP_002505614.1; XM_002505568.1.
DR AlphaFoldDB; C1EFK8; -.
DR STRING; 296587.C1EFK8; -.
DR GeneID; 8248776; -.
DR KEGG; mis:MICPUN_69585; -.
DR eggNOG; KOG2553; Eukaryota.
DR InParanoid; C1EFK8; -.
DR OMA; CDARTYT; -.
DR OrthoDB; 12038at2759; -.
DR Proteomes; UP000002009; Chromosome 13.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IEA:UniProt.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:InterPro.
DR CDD; cd02568; PseudoU_synth_PUS1_PUS2; 1.
DR Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 2.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR041708; PUS1/PUS2-like.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR NCBIfam; TIGR00071; hisT_truA; 1.
DR PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR PANTHER; PTHR11142:SF4; PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Reference proteome {ECO:0000313|Proteomes:UP000002009};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 163..249
FT /note="Pseudouridine synthase I TruA alpha/beta"
FT /evidence="ECO:0000259|Pfam:PF01416"
FT ACT_SITE 64
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-1"
FT BINDING 120
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-2"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ACO66872.1"
FT NON_TER 294
FT /evidence="ECO:0000313|EMBL:ACO66872.1"
SQ SEQUENCE 294 AA; 33151 MW; 96CAD11D38D00F80 CRC64;
RGKKRKIAAF LAYVGKGYQG FQRNPGARTI EDELERAIVA AGGISADNAG DFTKVQWTRA
ARTDKGVSAV GQCIALRMVL DPPGVLERIN AALPEGFKIF GYKRVTGGFN AKTMCDRRRY
EYVIPTCAFD PRRCLPRRED DKEAKAFVFD EELRARVNKI LGNYCGTHNF HNYTVRVDPN
DAAAMRYIIS FECGEPFVID GVEFVRTTVV GQSFMLHQIR KLIGTMLCVV RGYLAEEDQI
FALKTKESCS HEPLALDEYA DDVEAFKKSH IYPHMASTEK EEGTMEAWVR MLPL
//
