ID C1N882_MICPC Unreviewed; 446 AA.
AC C1N882;
DT 26-MAY-2009, integrated into UniProtKB/TrEMBL.
DT 26-MAY-2009, sequence version 1.
DT 27-MAR-2024, entry version 47.
DE SubName: Full=Predicted protein {ECO:0000313|EMBL:EEH51658.1};
DE Flags: Fragment;
GN ORFNames=MICPUCDRAFT_2776 {ECO:0000313|EMBL:EEH51658.1};
OS Micromonas pusilla (strain CCMP1545) (Picoplanktonic green alga).
OC Eukaryota; Viridiplantae; Chlorophyta; Mamiellophyceae; Mamiellales;
OC Mamiellaceae; Micromonas.
OX NCBI_TaxID=564608 {ECO:0000313|Proteomes:UP000001876};
RN [1] {ECO:0000313|EMBL:EEH51658.1, ECO:0000313|Proteomes:UP000001876}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCMP1545 {ECO:0000313|EMBL:EEH51658.1,
RC ECO:0000313|Proteomes:UP000001876};
RX PubMed=19359590; DOI=10.1126/science.1167222;
RA Worden A.Z., Lee J.H., Mock T., Rouze P., Simmons M.P., Aerts A.L.,
RA Allen A.E., Cuvelier M.L., Derelle E., Everett M.V., Foulon E.,
RA Grimwood J., Gundlach H., Henrissat B., Napoli C., McDonald S.M.,
RA Parker M.S., Rombauts S., Salamov A., Von Dassow P., Badger J.H.,
RA Coutinho P.M., Demir E., Dubchak I., Gentemann C., Eikrem W., Gready J.E.,
RA John U., Lanier W., Lindquist E.A., Lucas S., Mayer K.F., Moreau H.,
RA Not F., Otillar R., Panaud O., Pangilinan J., Paulsen I., Piegu B.,
RA Poliakov A., Robbens S., Schmutz J., Toulza E., Wyss T., Zelensky A.,
RA Zhou K., Armbrust E.V., Bhattacharya D., Goodenough U.W., Van de Peer Y.,
RA Grigoriev I.V.;
RT "Green evolution and dynamic adaptations revealed by genomes of the marine
RT picoeukaryotes Micromonas.";
RL Science 324:268-272(2009).
CC -!- FUNCTION: Catalytic subunit of an S-adenosyl-L-methionine-dependent
CC tRNA methyltransferase complex that mediates the methylation of the
CC guanosine nucleotide at position 10 (m2G10) in tRNAs.
CC {ECO:0000256|ARBA:ARBA00002425}.
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DR EMBL; GG663750; EEH51658.1; -; Genomic_DNA.
DR RefSeq; XP_003064036.1; XM_003063990.1.
DR AlphaFoldDB; C1N882; -.
DR STRING; 564608.C1N882; -.
DR GeneID; 9689665; -.
DR KEGG; mpp:MICPUCDRAFT_2776; -.
DR eggNOG; KOG2671; Eukaryota.
DR OMA; QAFNKWS; -.
DR OrthoDB; 5488599at2759; -.
DR Proteomes; UP000001876; Unassembled WGS sequence.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR000241; RlmKL-like_Mtase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR016691; tRNA_mtfrase_TRM11.
DR PANTHER; PTHR13370; RNA METHYLASE-RELATED; 1.
DR PANTHER; PTHR13370:SF3; TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG; 1.
DR Pfam; PF01170; UPF0020; 1.
DR PIRSF; PIRSF017259; tRNA_mtfrase_TRM11; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
DR PROSITE; PS51627; SAM_MT_TRM11; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000001876};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022555};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555}.
FT DOMAIN 196..246
FT /note="UPF0020"
FT /evidence="ECO:0000259|Pfam:PF01170"
FT REGION 310..354
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 330..348
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:EEH51658.1"
FT NON_TER 446
FT /evidence="ECO:0000313|EMBL:EEH51658.1"
SQ SEQUENCE 446 AA; 49234 MW; 706E159D016AE1A1 CRC64;
WYLCYFMHRH LDFRRAEFQA LADLNGFGDA VEWRAPFGGV EHSPFHYVRL PSDDAARAIM
KRSILTKGIV ELWGEGNDQD ELNAAIAAFD AETKARYTAE GSTFKVELED FGKTLHGTRD
DNWGPAVRRI DALKPAVEFN GRARMKGAEH LFWSIESAEG NDLRGIPSDI PSRHYFGRVV
AASAARAAIN TYDLKKRRYL GPTSMDVEMS LIMANMVHAG PGRVVWDPFC GTGSVLIAAA
HFGAMTMGSD IDVRRVKDKR TGEHVDVWTN FKDYALPPPI ALLRADLHKP PFRGAAASGA
ASERGACEGH LQGVVGDPPY GVRAGGRKSG GRKRLPDGTA RAVPEQHREN HIPSTVPYPF
HECMDDLMEQ AARLLSVGGR LSFFVPGETG PNTTPVPSHP MLTLRASSMQ LLSTRWGRRL
VTFEKTKKYD HEAAEEAKAA LTEARK
//
