ID C2BGF5_9FIRM Unreviewed; 466 AA.
AC C2BGF5;
DT 16-JUN-2009, integrated into UniProtKB/TrEMBL.
DT 16-JUN-2009, sequence version 1.
DT 27-MAR-2024, entry version 53.
DE RecName: Full=6-phospho-beta-galactosidase {ECO:0000256|RuleBase:RU004469};
DE EC=3.2.1.85 {ECO:0000256|RuleBase:RU004469};
GN Name=lacG {ECO:0000313|EMBL:EEI86082.1};
GN ORFNames=HMPREF0072_1425 {ECO:0000313|EMBL:EEI86082.1};
OS Anaerococcus lactolyticus ATCC 51172.
OC Bacteria; Bacillota; Tissierellia; Tissierellales; Peptoniphilaceae;
OC Anaerococcus.
OX NCBI_TaxID=525254 {ECO:0000313|EMBL:EEI86082.1, ECO:0000313|Proteomes:UP000005984};
RN [1] {ECO:0000313|EMBL:EEI86082.1, ECO:0000313|Proteomes:UP000005984}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51172 {ECO:0000313|EMBL:EEI86082.1,
RC ECO:0000313|Proteomes:UP000005984};
RA Qin X., Bachman B., Battles P., Bell A., Bess C., Bickham C., Chaboub L.,
RA Chen D., Coyle M., Deiros D.R., Dinh H., Forbes L., Fowler G.,
RA Francisco L., Fu Q., Gubbala S., Hale W., Han Y., Hemphill L.,
RA Highlander S.K., Hirani K., Hogues M., Jackson L., Jakkamsetti A.,
RA Javaid M., Jiang H., Korchina V., Kovar C., Lara F., Lee S., Mata R.,
RA Mathew T., Moen C., Morales K., Munidasa M., Nazareth L., Ngo R.,
RA Nguyen L., Okwuonu G., Ongeri F., Patil S., Petrosino J., Pham C., Pham P.,
RA Pu L.-L., Puazo M., Raj R., Reid J., Rouhana J., Saada N., Shang Y.,
RA Simmons D., Thornton R., Warren J., Weissenberger G., Zhang J., Zhang L.,
RA Zhou C., Zhu D., Muzny D., Worley K., Gibbs R.;
RL Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 6-phospho-beta-D-galactoside + H2O = an alcohol + D-
CC galactose 6-phosphate; Xref=Rhea:RHEA:24568, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:30879, ChEBI:CHEBI:58534, ChEBI:CHEBI:91004; EC=3.2.1.85;
CC Evidence={ECO:0000256|RuleBase:RU004469};
CC -!- PATHWAY: Carbohydrate metabolism; lactose degradation; D-galactose 6-
CC phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1.
CC {ECO:0000256|RuleBase:RU004469}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family.
CC {ECO:0000256|ARBA:ARBA00010838}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEI86082.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; ABYO01000215; EEI86082.1; -; Genomic_DNA.
DR RefSeq; WP_004829343.1; NZ_GG666049.1.
DR AlphaFoldDB; C2BGF5; -.
DR STRING; 525254.HMPREF0072_1425; -.
DR eggNOG; COG2723; Bacteria.
DR HOGENOM; CLU_001859_1_3_9; -.
DR UniPathway; UPA00542; UER00605.
DR Proteomes; UP000005984; Unassembled WGS sequence.
DR GO; GO:0033920; F:6-phospho-beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0019512; P:lactose catabolic process via tagatose-6-phosphate; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR005928; 6P-beta-galactosidase.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR018120; Glyco_hydro_1_AS.
DR InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR NCBIfam; TIGR01233; lacG; 1.
DR PANTHER; PTHR10353:SF340; 6-PHOSPHO-BETA-GALACTOSIDASE 1; 1.
DR PANTHER; PTHR10353; GLYCOSYL HYDROLASE; 1.
DR Pfam; PF00232; Glyco_hydro_1; 1.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00572; GLYCOSYL_HYDROL_F1_1; 1.
DR PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU004468};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU004468};
KW Reference proteome {ECO:0000313|Proteomes:UP000005984}.
FT ACT_SITE 372
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10055"
SQ SEQUENCE 466 AA; 54011 MW; A340D9FF07CDC6F5 CRC64;
MKFKDDFIFG GATAAYQCEG ATKEDGKGLV CWDVFYEENN LYNPDPASGF YHEYKQDIDN
CVKFGINGIR VSIAWTRIFP EGSGKVNQKG VDHYHELFKY CLDNNVEPFV TLHHFDTPFE
LNKNGDFTDE KVREDYLNYA KFCLDEFSEV KKWASFNEIW PWSVNQFITG TFPPGNKYRF
DLAVENIHGM LTCHAKALNY FKDNNKEGEF GVIHSLETYY SIDENPENIE AAKRYDAIAN
GLCIEAVLAG GYSKETLDRV NYILEVNGFD KFVPNQADLA EIKKASPRID FLGINYYQAH
WMKEYNGENL HIHNGTGDKG SFKSQLKGMG EEVKRDDIPK TDWDWSIYPQ GLYDMIMRVS
AYDNCKKIYI TENGLGLKEE LNSEKTVNDD ARIDYVRDHL KAVLKANENG ANVAGYFLWS
LMDMFSWSNG YNKRYGFFFV DFDTLERYPK KSAYWWKELT ESKELN
//
