ID C2EFH9_9LACO Unreviewed; 282 AA.
AC C2EFH9;
DT 16-JUN-2009, integrated into UniProtKB/TrEMBL.
DT 16-JUN-2009, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=Probable cell wall amidase lytH {ECO:0000256|ARBA:ARBA00018811, ECO:0000256|PIRNR:PIRNR037730};
DE EC=3.5.1.- {ECO:0000256|PIRNR:PIRNR037730};
GN Name=amiC {ECO:0000313|EMBL:EEJ74732.1};
GN ORFNames=HMPREF0545_0401 {ECO:0000313|EMBL:EEJ74732.1};
OS Ligilactobacillus salivarius DSM 20555 = ATCC 11741.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Ligilactobacillus.
OX NCBI_TaxID=1423799 {ECO:0000313|EMBL:EEJ74732.1, ECO:0000313|Proteomes:UP000003531};
RN [1] {ECO:0000313|EMBL:EEJ74732.1, ECO:0000313|Proteomes:UP000003531}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 11741 {ECO:0000313|EMBL:EEJ74732.1,
RC ECO:0000313|Proteomes:UP000003531};
RA Qin X., Bachman B., Battles P., Bell A., Bess C., Bickham C., Chaboub L.,
RA Chen D., Coyle M., Deiros D.R., Dinh H., Forbes L., Fowler G.,
RA Francisco L., Fu Q., Gubbala S., Hale W., Han Y., Hemphill L.,
RA Highlander S.K., Hirani K., Hogues M., Jackson L., Jakkamsetti A.,
RA Javaid M., Jiang H., Korchina V., Kovar C., Lara F., Lee S., Mata R.,
RA Mathew T., Moen C., Morales K., Munidasa M., Nazareth L., Ngo R.,
RA Nguyen L., Okwuonu G., Ongeri F., Patil S., Petrosino J., Pham C., Pham P.,
RA Pu L.-L., Puazo M., Raj R., Reid J., Rouhana J., Saada N., Shang Y.,
RA Simmons D., Thornton R., Warren J., Weissenberger G., Zhang J., Zhang L.,
RA Zhou C., Zhu D., Muzny D., Worley K., Gibbs R.;
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Probably involved in cell-wall metabolism.
CC {ECO:0000256|PIRNR:PIRNR037730}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613,
CC ECO:0000256|PIRNR:PIRNR037730}.
CC -!- SIMILARITY: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.
CC {ECO:0000256|ARBA:ARBA00010860, ECO:0000256|PIRNR:PIRNR037730}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEJ74732.1}.
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DR EMBL; ACGT01000002; EEJ74732.1; -; Genomic_DNA.
DR RefSeq; WP_003700256.1; NZ_GG693223.1.
DR AlphaFoldDB; C2EFH9; -.
DR HOGENOM; CLU_014322_1_1_9; -.
DR Proteomes; UP000003531; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008745; F:N-acetylmuramoyl-L-alanine amidase activity; IEA:InterPro.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0009253; P:peptidoglycan catabolic process; IEA:InterPro.
DR CDD; cd02696; MurNAc-LAA; 1.
DR Gene3D; 2.30.30.40; SH3 Domains; 1.
DR Gene3D; 3.40.630.40; Zn-dependent exopeptidases; 1.
DR InterPro; IPR017273; LytH.
DR InterPro; IPR002508; MurNAc-LAA_cat.
DR InterPro; IPR003646; SH3-like_bac-type.
DR PANTHER; PTHR30404; N-ACETYLMURAMOYL-L-ALANINE AMIDASE; 1.
DR PANTHER; PTHR30404:SF0; N-ACETYLMURAMOYL-L-ALANINE AMIDASE AMIC; 1.
DR Pfam; PF01520; Amidase_3; 1.
DR Pfam; PF08239; SH3_3; 1.
DR PIRSF; PIRSF037730; CWA_LytH_prd; 1.
DR SMART; SM00646; Ami_3; 1.
DR SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE 3: Inferred from homology;
KW Cell wall biogenesis/degradation {ECO:0000256|PIRNR:PIRNR037730};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR037730, ECO:0000313|EMBL:EEJ74732.1};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Secreted {ECO:0000256|ARBA:ARBA00022525, ECO:0000256|PIRNR:PIRNR037730};
KW Signal {ECO:0000256|ARBA:ARBA00022729};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 12..30
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 167..276
FT /note="MurNAc-LAA"
FT /evidence="ECO:0000259|SMART:SM00646"
SQ SEQUENCE 282 AA; 32347 MW; 06ADE369DC235EFE CRC64;
MKDYKKILLS RIDLKILYLI IIILFSILTF RTVHYIRQVP LINKAIIYQS YNTSSKNLGT
LNMGDRVTVL STKYHWKKVK TSEGEVGWIQ DWNFRQQNKI TSLSDATIVI DAGHGGSDSG
ALSRTNKNEK TYTLIYAKKL AERLRKAGAM VYMTRDDDSF VSLNSRPQLA ENLHADAFIS
IHFDSAPENN MGSGYTTYYY HKKTSLRLAQ DINSKLKYLK LENRGVEFGD FLVIRENTVP
AVLLEMGYIN SDRDFERITS TSYQDSVADD IKQGLDTYFN QN
//
