ID C3VPE7_CVMA5 Unreviewed; 2733 AA.
AC C3VPE7;
DT 16-JUN-2009, integrated into UniProtKB/TrEMBL.
DT 16-JUN-2009, sequence version 1.
DT 27-MAR-2024, entry version 87.
DE RecName: Full=ORF1ab polyprotein {ECO:0000256|PROSITE-ProRule:PRU01344};
DE Flags: Fragment;
OS Murine coronavirus (strain A59) (MHV-A59) (Murine hepatitis virus).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC Betacoronavirus; Embecovirus; Murine coronavirus.
OX NCBI_TaxID=11142 {ECO:0000313|EMBL:ACO72882.1, ECO:0000313|Proteomes:UP000180349};
OH NCBI_TaxID=10090; Mus musculus (Mouse).
RN [1] {ECO:0000313|EMBL:ACO72882.1, ECO:0000313|Proteomes:UP000180349}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A59 {ECO:0000313|EMBL:ACO72882.1};
RX PubMed=8392595;
RA Gombold J.L., Hingley S.T., Weiss S.R.;
RT "Fusion-defective mutants of mouse hepatitis virus A59 contain a mutation
RT in the spike protein cleavage signal.";
RL J. Virol. 67:4504-4512(1993).
CC -!- FUNCTION: The replicase polyprotein of coronaviruses is a
CC multifunctional protein: it contains the activities necessary for the
CC transcription of negative stranded RNA, leader RNA, subgenomic mRNAs
CC and progeny virion RNA as well as proteinases responsible for the
CC cleavage of the polyprotein into functional products.
CC {ECO:0000256|ARBA:ARBA00003368}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
CC -!- SUBCELLULAR LOCATION: Host cytoplasm, host perinuclear region
CC {ECO:0000256|ARBA:ARBA00004407}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the coronaviruses polyprotein 1ab family.
CC {ECO:0000256|ARBA:ARBA00008087, ECO:0000256|PROSITE-ProRule:PRU01299}.
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DR EMBL; FJ884686; ACO72882.1; -; Genomic_RNA.
DR Proteomes; UP000180349; Genome.
DR GO; GO:0044220; C:host cell perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000175; F:3'-5'-RNA exonuclease activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0004483; F:mRNA (nucleoside-2'-O-)-methyltransferase activity; IEA:InterPro.
DR GO; GO:0004482; F:mRNA 5'-cap (guanine-N7-)-methyltransferase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR CDD; cd21409; 1B_cv_Nsp13-like; 1.
DR CDD; cd21722; betaCoV_Nsp13-helicase; 1.
DR CDD; cd21659; betaCoV_Nsp14; 1.
DR CDD; cd20762; capping_2-OMTase_Nidovirales; 1.
DR CDD; cd21593; HCoV_HKU1-like_RdRp; 1.
DR CDD; cd21167; M_alpha_beta_cv_Nsp15-like; 1.
DR CDD; cd21161; NendoU_cv_Nsp15-like; 1.
DR CDD; cd21171; NTD_alpha_betaCoV_Nsp15-like; 1.
DR CDD; cd21689; stalk_CoV_Nsp13-like; 1.
DR CDD; cd21401; ZBD_cv_Nsp13-like; 1.
DR Gene3D; 3.40.50.11580; -; 1.
DR Gene3D; 3.30.160.820; Nsp15 N-terminal domain-like; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR046440; AV_NSP11N_COV_NSP15M.
DR InterPro; IPR043608; CoV_NSP15_M.
DR InterPro; IPR043606; CoV_NSP15_N.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR037227; EndoU-like.
DR InterPro; IPR046435; N7_MTase_CoV.
DR InterPro; IPR043609; NendoU_nidovirus.
DR InterPro; IPR044863; NIRAN.
DR InterPro; IPR046438; NIV_2_O_MTASE.
DR InterPro; IPR046436; NIV_EXON.
DR InterPro; IPR047570; NSP12_IF_CoV.
DR InterPro; IPR044343; NSP13_1B_dom_CoV.
DR InterPro; IPR048673; NSP13_stalk_CoV.
DR InterPro; IPR048672; NSP13_ZBD_CoV.
DR InterPro; IPR027352; NSP13_ZBD_CoV-like.
DR InterPro; IPR044315; NSP14_betaCoV.
DR InterPro; IPR009466; NSP14_CoV.
DR InterPro; IPR044330; NSP15_alpha_betaCoV_N.
DR InterPro; IPR044322; NSP15_M_alpha_beta_CoV.
DR InterPro; IPR043174; NSP15_middle_sf.
DR InterPro; IPR042515; NSP15_N_CoV.
DR InterPro; IPR044401; NSP15_NendoU_CoV.
DR InterPro; IPR009461; NSP16_CoV-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR046441; RdRp_CoV.
DR InterPro; IPR044347; RdRp_HCoV_HKU1-like.
DR InterPro; IPR009469; RdRp_N_CoV.
DR InterPro; IPR001205; RNA-dir_pol_C.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR43788; DNA2/NAM7 HELICASE FAMILY MEMBER; 1.
DR PANTHER; PTHR43788:SF8; HELICASE WITH ZINC FINGER 2; 1.
DR Pfam; PF13087; AAA_12; 1.
DR Pfam; PF13604; AAA_30; 1.
DR Pfam; PF06471; CoV_ExoN; 1.
DR Pfam; PF06460; CoV_Methyltr_2; 1.
DR Pfam; PF20631; CoV_NSP13_1B; 1.
DR Pfam; PF20633; CoV_NSP13_stalk; 1.
DR Pfam; PF20632; CoV_NSP13_ZBD; 1.
DR Pfam; PF19215; CoV_NSP15_C; 1.
DR Pfam; PF19216; CoV_NSP15_M; 1.
DR Pfam; PF19219; CoV_NSP15_N; 1.
DR Pfam; PF06478; CoV_RPol_N; 1.
DR Pfam; PF00680; RdRP_1; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF142877; EndoU-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51961; AV_NSP11N_COV_NSP15M; 1.
DR PROSITE; PS51954; COV_N7_MTASE; 1.
DR PROSITE; PS52000; COV_NSP12_IF; 1.
DR PROSITE; PS51948; COV_NSP12_RDRP; 1.
DR PROSITE; PS51960; COV_NSP15_NTD; 1.
DR PROSITE; PS51653; CV_ZBD; 1.
DR PROSITE; PS51958; NENDOU; 1.
DR PROSITE; PS51947; NIRAN; 1.
DR PROSITE; PS51955; NIV_2_O_MTASE; 1.
DR PROSITE; PS51953; NIV_EXON; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759, ECO:0000256|PROSITE-
KW ProRule:PRU01303}; Exonuclease {ECO:0000256|PROSITE-ProRule:PRU01298};
KW Host membrane {ECO:0000256|ARBA:ARBA00022870};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU01303}; Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01299};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|PROSITE-
KW ProRule:PRU01298}; Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Protease {ECO:0000256|ARBA:ARBA00022807};
KW Ribosomal frameshifting {ECO:0000256|ARBA:ARBA00022758};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484};
KW Thiol protease {ECO:0000256|ARBA:ARBA00022807};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01299}; Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Zinc {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-ProRule:PRU00986};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00986}.
FT DOMAIN 17..272
FT /note="NiRAN"
FT /evidence="ECO:0000259|PROSITE:PS51947"
FT DOMAIN 273..371
FT /note="Nsp12 Interface"
FT /evidence="ECO:0000259|PROSITE:PS52000"
FT DOMAIN 372..939
FT /note="Nsp12 RNA-dependent RNA polymerase"
FT /evidence="ECO:0000259|PROSITE:PS51948"
FT DOMAIN 619..781
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50507"
FT DOMAIN 940..1023
FT /note="CV ZBD"
FT /evidence="ECO:0000259|PROSITE:PS51653"
FT DOMAIN 1195..1546
FT /note="(+)RNA virus helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51657"
FT DOMAIN 1610..1825
FT /note="ExoN"
FT /evidence="ECO:0000259|PROSITE:PS51953"
FT DOMAIN 1834..2060
FT /note="N7-MTase"
FT /evidence="ECO:0000259|PROSITE:PS51954"
FT DOMAIN 2061..2121
FT /note="Nsp15 N-terminal oligomerization"
FT /evidence="ECO:0000259|PROSITE:PS51960"
FT DOMAIN 2122..2242
FT /note="AV-Nsp11N/CoV-Nsp15M"
FT /evidence="ECO:0000259|PROSITE:PS51961"
FT DOMAIN 2292..2431
FT /note="NendoU"
FT /evidence="ECO:0000259|PROSITE:PS51958"
FT DOMAIN 2436..2730
FT /note="Nidovirus-type SAM-dependent 2'-O-MTase"
FT /evidence="ECO:0000259|PROSITE:PS51955"
FT REGION 1947..1961
FT /note="GpppA-binding"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01299"
FT ACT_SITE 1628
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01298"
FT ACT_SITE 1630
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01298"
FT ACT_SITE 1729
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01298"
FT ACT_SITE 1806
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01298"
FT ACT_SITE 1811
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01298"
FT ACT_SITE 2322
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01303"
FT ACT_SITE 2337
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01303"
FT ACT_SITE 2377
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01303"
FT BINDING 1869..1875
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01299"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ACO72882.1"
SQ SEQUENCE 2733 AA; 309167 MW; 55B4CCB931745D46 CRC64;
LFLCRHRLPV SVKRHELFKR VRGTSVNARL VPCASGLDTD VQLRAFDICN ANRAGIGLYY
KVNCCRFQRV DEDGNKLDKF FVVKRTNLEV YNKEKECYEL TKECGVVAEH EFFTFDVEGS
RVPHIVRKDL SKFTMLDLCY ALRHFDRNDC STLKEILLTY AECEESYFQK KDWYDFVENP
DIINVYKKLG PIFNRALLNT AKFADALVEA GLVGVLTLDN QDLYGQWYDF GDFVKTVPGC
GVAVADSYYS YMMPMLTMCH ALDSELFVNG TYREFDLVQY DFTDFKLELF TKYFKHWSMT
YHPNTCECED DRCIIHCANF NILFSMVLPK TCFGPLVRQI FVDGVPFVVS IGYHYKELGV
VMNMDVDTHR YRLSLKDLLL YAADPALHVA SASALLDLRT CCFSVAAITS GVKFQTVKPG
NFNQDFYEFI LSKGLLKEGS SVDLKHFFFT QDGNAAITDY NYYKYNLPTM VDIKQLLFVL
EVVNKYFEIY EGGCTPATQV IVNNYDKSAG YPFNKFGKAR LYYEALSFEE QDEIYAYTKR
NVLPTLTQMN LKYAISAKNR ARTVAGVSIL STMTGRMFHQ KCLKSIAATR GVPVVIGTTK
FYGGWDDMLR RLIKDVDSPV LMGWDYPKCD RAMPNILRIV SSLVLARKHD SCCSHTDRFY
RLANECAQVL SEIVMCGGCY YVKPGGTSSG DATTAFANSV FNICQAVSAN VCSLMACNGH
KIEDLSIREL QKRLYSNVYR ADHVDPAFVS EYYEFLNKHF SMMILSDDGV VCYNSEFASK
GYIANISAFQ QVLYYQNNVF MSEAKCWVET DIEKGPHEFC SQHTMLVKMD GDEVYLPYPD
PSRILGAGCF VDDLLKTDSV LLIERFVSLA IDAYPLVYHE NPEYQNVFRV YLEYIKKLYN
DLGNQILDSY SVILSTCDGQ KFTDETFYKN MYLRSAVLQS VGACVVCSSQ TSLRCGSCIR
KPLLCCKCAY DHVMSTDHKY VLSVSPYVCN SPGCDVNDVT KLYLGGMSYY CEDHKPQYSF
KLVMNGMVFG LYKQSCTGSP YIEDFNKIAS CKWTEVDDYV LANECTERLK LFAAETQKAT
EEAFKQCYAS ATIREIVSDR ELILSWEIGK VRPPLNKNYV FTGYHFTNNG KTVLGEYVFD
KSELTNGVYY RATTTYKLSV GDVFILTSHA VSSLSAPTLV PQENYTSIRF ASVYSVPETF
QNNVPNYQHI GMKRYCTVQG PPGTGKSHLA IGLAVYYCTA RVVYTAASHA AVDALCEKAH
KFLNINDCTR IVPAKVRVDC YDKFKVNDTT RKYVFTTINA LPELVTDIIV VDEVSMLTNY
ELSVINSRVR AKHYVYIGDP AQLPAPRVLL NKGTLEPRYF NSVTKLMCCL GPDIFLGTCY
RCPKEIVDTV SALVYNNKLK AKNDNSSMCF KVYYKGQTTH ESSSAVNMQQ IHLISKFLKA
NPSWSNAVFI SPYNSQNYVA KRVLGLQTQT VDSAQGSEYD FVIYSQTAET AHSVNVNRFN
VAITRAKKGI LCVMSSMQLF ESLNFTTLTL DKINNPRLQC TTNLFKDCSR SYVGYHPAHA
PSFLAVDDKY KVGGDLAVCL NVADSAVTYS RLISLMGFKL DLTLDGYCKL FITRDEAIKR
VRAWVGFDAE GAHAIRDSIG TNFPLQLGFS TGIDFVVEAT GMFAERDGYV FKKAAARAPP
GEQFKHLIPL MSRGQKWDVV RIRIVQMLSD HLADLADSVV LVTWAASFEL TCLRYFAKVG
REVVCSVCTK RATCFNSRTG YYGCWRHSYS CDYLYNPLIV DIQQWGYTGS LTSNHDPICS
VHKGAHVASS DAIMTRCLAV HDCFCKSVNW NLEYPIISNE VSVNTSCRLL QRVMFRAAML
CNRYDVCYDI GNPKGLACVK GYDFKFYDAS PVVKSVKQFV YKYEAHKDQF LDGLCMFWNC
NVDKYPANAV VCRFDTRVLN KLNLPGCNGG SLYVNKHAFH TSPFTRAAFE NLKPMPFFYY
SDTPCVYMEG MESKQVDYVP LRSATCITRC NLGGAVCLKH AEEYREYLES YNTATTAGFT
FWVYKTFDFY NLWNTFTRLQ SLENVVYNLV NAGHFDGRAG ELPCAVIGEK VIAKIQNEDV
VVFKNNTPFP TNVAVELFAK RSIRPHPELK LFRNLNIDVC WSHVLWDYAK DSVFCSSTYK
VCKYTDLQCI ESLNVLFDGR DNGALEAFKK CRNGVYINTT KIKSLSMIKG PQRADLNGVV
VEKVGDSDVE FWFAVRKDGD DVIFSRTGSL EPSHYRSPQG NPGGNRVGDL SGNEALARGT
IFTQSRLLSS FTPRSEMEKD FMDLDDDVFI AKYSLQDYAF EHVVYGSFNQ KIIGGLHLLI
GLARRQQKSN LVIQEFVTYD SSIHSYFITD ENSGSSKSVC TVIDLLLDDF VDIVKSLNLK
CVSKVVNVNV DFKDFQFMLW CNEEKVMTFY PRLQAAADWK PGYVMPVLYK YLESPLERVN
LWNYGKPITL PTGCMMNVAK YTQLCQYLST TTLAVPANMR VLHLGAGSDK GVAPGSAVLR
QWLPAGSILV DNDVNPFVSD SVASYYGNCI TLPFDCQWDL IISDMYDPLT KNIGEYNVSK
DGFFTYLCHL IRDKLALGGS VAIKITEFSW NAELYSLMGK FAFWTIFCTN VNASSSEGFL
IGINWLNKTR TEIDGKTMHA NYLFWRNSTM WNGGAYSLFD MSKFPLKAAG TAVVSLKPDQ
INDLVLSLIE KGKLLVRDTR KEVFVGDSLV NVK
//
