ID C3Y381_BRAFL Unreviewed; 343 AA.
AC C3Y381;
DT 28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 28-JUL-2009, sequence version 1.
DT 24-JAN-2024, entry version 58.
DE RecName: Full=S-adenosylmethionine-dependent nucleotide dehydratase RSAD2 {ECO:0000256|ARBA:ARBA00035038};
DE AltName: Full=Radical S-adenosyl methionine domain-containing protein 2 {ECO:0000256|ARBA:ARBA00035040};
DE AltName: Full=Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible {ECO:0000256|ARBA:ARBA00035042};
GN ORFNames=BRAFLDRAFT_106305 {ECO:0000313|EMBL:EEN65148.1};
OS Branchiostoma floridae (Florida lancelet) (Amphioxus).
OC Eukaryota; Metazoa; Chordata; Cephalochordata; Leptocardii; Amphioxiformes;
OC Branchiostomatidae; Branchiostoma.
OX NCBI_TaxID=7739;
RN [1] {ECO:0000313|EMBL:EEN65148.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S238N-H82 {ECO:0000313|EMBL:EEN65148.1};
RC TISSUE=Testes {ECO:0000313|EMBL:EEN65148.1};
RX PubMed=18563158; DOI=10.1038/nature06967;
RG US DOE Joint Genome Institute (JGI-PGF);
RA Putnam N.H., Butts T., Ferrier D.E.K., Furlong R.F., Hellsten U.,
RA Kawashima T., Robinson-Rechavi M., Shoguchi E., Terry A., Yu J.-K.,
RA Benito-Gutierrez E.L., Dubchak I., Garcia-Fernandez J., Gibson-Brown J.J.,
RA Grigoriev I.V., Horton A.C., de Jong P.J., Jurka J., Kapitonov V.V.,
RA Kohara Y., Kuroki Y., Lindquist E., Lucas S., Osoegawa K., Pennacchio L.A.,
RA Salamov A.A., Satou Y., Sauka-Spengler T., Schmutz J., Shin-I T.,
RA Toyoda A., Bronner-Fraser M., Fujiyama A., Holland L.Z., Holland P.W.H.,
RA Satoh N., Rokhsar D.S.;
RT "The amphioxus genome and the evolution of the chordate karyotype.";
RL Nature 453:1064-1071(2008).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004397}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004397}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004397}. Membrane
CC {ECO:0000256|ARBA:ARBA00004287}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004287}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004287}.
CC -!- SIMILARITY: Belongs to the radical SAM superfamily. RSAD2 family.
CC {ECO:0000256|ARBA:ARBA00035008}.
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DR EMBL; GG666483; EEN65148.1; -; Genomic_DNA.
DR RefSeq; XP_002609138.1; XM_002609092.1.
DR AlphaFoldDB; C3Y381; -.
DR STRING; 7739.C3Y381; -.
DR eggNOG; ENOG502QQMH; Eukaryota.
DR InParanoid; C3Y381; -.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IBA:GO_Central.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR026372; RSAD2.
DR InterPro; IPR007197; rSAM.
DR NCBIfam; TIGR04278; viperin; 1.
DR NCBIfam; NF038283; viperin_w_prok; 1.
DR PANTHER; PTHR21339; RADICAL S-ADENOSYL METHIONINE DOMAIN-CONTAINING PROTEIN 2; 1.
DR PANTHER; PTHR21339:SF0; RADICAL S-ADENOSYL METHIONINE DOMAIN-CONTAINING PROTEIN 2; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01088; antiviral_proteins; 1.
DR SFLD; SFLDG01067; SPASM/twitch_domain_containing; 1.
DR SFLD; SFLDF00318; Viperin; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Immunity {ECO:0000256|ARBA:ARBA00022859};
KW Innate immunity {ECO:0000256|ARBA:ARBA00022588};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 62..282
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 343 AA; 39004 MW; 6EFE6DB4F037F349 CRC64;
MTTVTCTNVT LPLAVVFILG VLAVFFRRVN KFTQVAWDVL RGEYSVAAPS LPSGPRKTPP
GTVVPTSVNY HFTRQCNYRC GFCFHTAKTS FVLPLDTAKK GLRLLVEAGM EKVNFSGGEP
FIKDRGRYVG ELVRFCKCEL GLPSVSIVSN GSLITEDWFG KYGEWLDILA ISCDSFDEET
NRRIGRHQTG KDHLRCLRQV RTWCQQYKVA FKINSVVNRH NIVEDMSSEI MELNPIRWKV
FQCLLIGGEN AGPAALRNAE DMVITDQEFD QFVRRHEQVT CLVPESNEKM RFLDCRNGRK
EPSRSLLDVG VKGAIQFSGF DEKMFFKRGG KYTWSKADLQ LDW
//
