ID C4ICB4_CLOBU Unreviewed; 548 AA.
AC C4ICB4;
DT 07-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 07-JUL-2009, sequence version 1.
DT 24-JAN-2024, entry version 43.
DE SubName: Full=Beta-xylosidase (1,4-beta-D-xylan xylohydrolase) (Xylan1,4-beta-xylosidase) {ECO:0000313|EMBL:EEP56585.1};
DE EC=3.2.1.37 {ECO:0000313|EMBL:EEP56585.1};
GN ORFNames=CLP_0789 {ECO:0000313|EMBL:EEP56585.1};
OS Clostridium butyricum E4 str. BoNT E BL5262.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=632245 {ECO:0000313|EMBL:EEP56585.1, ECO:0000313|Proteomes:UP000003081};
RN [1] {ECO:0000313|EMBL:EEP56585.1, ECO:0000313|Proteomes:UP000003081}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=E4 str. BoNT E BL5262 {ECO:0000313|Proteomes:UP000003081};
RA Shrivastava S., Brinkac L.B., Brown J.L., Bruce D.B., Detter C.,
RA Green L.D., Munk C.A., Rogers Y.C., Tapia R., Sims D.R., Smith L.A.,
RA Smith T.J., Sutton G., Brettin T.;
RL Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEP56585.1}.
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DR EMBL; ACOM01000001; EEP56585.1; -; Genomic_DNA.
DR RefSeq; WP_003413705.1; NZ_ACOM01000001.1.
DR AlphaFoldDB; C4ICB4; -.
DR STRING; 1492.ATN24_18875; -.
DR eggNOG; COG3507; Bacteria.
DR HOGENOM; CLU_016508_2_1_9; -.
DR Proteomes; UP000003081; Unassembled WGS sequence.
DR GO; GO:0009044; F:xylan 1,4-beta-xylosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd09000; GH43_SXA-like; 1.
DR Gene3D; 2.60.120.200; -; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR041542; GH43_C2.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR Pfam; PF17851; GH43_C2; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW Reference proteome {ECO:0000313|Proteomes:UP000003081}.
FT DOMAIN 343..539
FT /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT /evidence="ECO:0000259|Pfam:PF17851"
FT ACT_SITE 13
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 188
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 128
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 548 AA; 63278 MW; 456404CAB30C66DC CRC64;
MIKNPILPGF NPDPCICRKG DDYYIAVSSF EWFPGIPIYH SKDLKNWELY THVLTDDKMV
DLKKLPSAKG IWAPCLTYCE KEDLFYVVYG VMNSMNARYF DVDNYIISAK DIKGPWSEPV
YIHSSGFDAS IFHDDDGKKY IVSLEWETRE GYEKPGAICI VEYSPKKKEI IGYPQKIWSG
GTDRGCIEAP HLTKRGDYYY IMCAEGGTGY GHSVTMGRSK NILGPFEKDP KNPIVTSIPG
DFNERHDPDH LKPKYFNPKS VLQKSGHGSY VETPLGEVYL VHLTARPFVP ELRCTLGRET
AIQKMKWTED GWLRMEDGSN FAKQYVDESN IKEYRVEKCP DFDDFDKGEL GLQYYSPRIM
PSSFADVKAR PGYVRIRGQE SRCSLNKVSI LARKLTSVYA VVTTKMEFIP YVHQHSAGLI
MYYDNMNYIY LRKYYSDTLK HSAISVIHLE NGEKTEFINT RTRVEDSPIY FRLVIEGRKS
HFEWSYDGTN YSVIGKIFDT TKFSDEYCKY GEFTGTFVGI TCADRVLHKH YADFDFFEYI
ADEDKNVE
//
