ID C4IFI8_CLOBU Unreviewed; 385 AA.
AC C4IFI8;
DT 07-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 07-JUL-2009, sequence version 1.
DT 27-MAR-2024, entry version 56.
DE SubName: Full=Thiazole biosynthesis protein ThiH {ECO:0000313|EMBL:EEP54321.1};
GN Name=thiH {ECO:0000313|EMBL:EEP54321.1};
GN ORFNames=CLP_1692 {ECO:0000313|EMBL:EEP54321.1};
OS Clostridium butyricum E4 str. BoNT E BL5262.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=632245 {ECO:0000313|EMBL:EEP54321.1, ECO:0000313|Proteomes:UP000003081};
RN [1] {ECO:0000313|EMBL:EEP54321.1, ECO:0000313|Proteomes:UP000003081}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=E4 str. BoNT E BL5262 {ECO:0000313|Proteomes:UP000003081};
RA Shrivastava S., Brinkac L.B., Brown J.L., Bruce D.B., Detter C.,
RA Green L.D., Munk C.A., Rogers Y.C., Tapia R., Sims D.R., Smith L.A.,
RA Smith T.J., Sutton G., Brettin T.;
RL Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEP54321.1}.
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DR EMBL; ACOM01000005; EEP54321.1; -; Genomic_DNA.
DR RefSeq; WP_003411197.1; NZ_ACOM01000005.1.
DR AlphaFoldDB; C4IFI8; -.
DR STRING; 1492.ATN24_11565; -.
DR eggNOG; COG0502; Bacteria.
DR HOGENOM; CLU_046249_1_0_9; -.
DR Proteomes; UP000003081; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0009228; P:thiamine biosynthetic process; IEA:InterPro.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR010722; BATS_dom.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR012726; ThiH.
DR InterPro; IPR034428; ThiH/NoCL/HydG-like.
DR NCBIfam; TIGR02351; thiH; 1.
DR PANTHER; PTHR43583; 2-IMINOACETATE SYNTHASE; 1.
DR PANTHER; PTHR43583:SF1; 2-IMINOACETATE SYNTHASE; 1.
DR Pfam; PF06968; BATS; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDF00301; 2-iminoacetate_synthase_(ThiH); 1.
DR SFLD; SFLDG01081; cleavage_of_the_Ca-Cb_bond_in; 1.
DR SMART; SM00876; BATS; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000003081};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 86..303
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 385 AA; 44046 MW; 207010E07072DA3C CRC64;
MEQRIDHMKY LPNMEVLEES NIMNEVITRM NSYDYENYTS EDVMTALNKD VLNPEDFAAL
LSPAAFPFLE QMAQRAKIET RKHFGNSINM FTPLYIANYC ENYCIYCGFN CHNKIKRARL
NAEEIEKEMQ VIAETGLQEI LLLTGESRSM SDVKYIGEAC KIAKKYFKVI GVEVYPMNSD
EYAYLHECGV DYVTVFQETY NSDKYETLHL AGHKRIFPYR LNAQERALKG GMRGVGFAAL
LGLDDFRKDA FATGMHAYLL QKKYPHAEIA FSCPRLRPII NNDNINPKDV HEPQLLQVMC
AYRIFMPYAA MTISTREQAH FRDNVIGLAA TKISAGVSVG IGGHASEEEK GDEQFEISDP
RNVDEVYNAI INHDLQPVMS DYVYV
//
