ID RNC_RICPU Reviewed; 227 AA.
AC C4K1R3;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 1.
DT 01-MAY-2013, entry version 29.
DE RecName: Full=Ribonuclease 3;
DE EC=3.1.26.3;
DE AltName: Full=Ribonuclease III;
DE Short=RNase III;
GN Name=rnc; OrderedLocusNames=RPR_04080;
OS Rickettsia peacockii (strain Rustic).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX NCBI_TaxID=562019;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Rustic;
RX PubMed=20027221; DOI=10.1371/journal.pone.0008361;
RA Felsheim R.F., Kurtti T.J., Munderloh U.G.;
RT "Genome sequence of the endosymbiont Rickettsia peacockii and
RT comparison with virulent Rickettsia rickettsii: identification of
RT virulence factors.";
RL PLoS ONE 4:E8361-E8361(2009).
CC -!- FUNCTION: Digests double-stranded RNA. Involved in the processing
CC of primary rRNA transcript to yield the immediate precursors to
CC the large and small rRNAs (23S and 16S). Also processes some
CC mRNAs, and tRNAs when they are encoded in the rRNA operon (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
CC phosphomonoester.
CC -!- COFACTOR: Mg(2+) (By similarity).
CC -!- SUBUNIT: Homodimer (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Contains 1 DRBM (double-stranded RNA-binding) domain.
CC -!- SIMILARITY: Contains 1 RNase III domain.
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DR EMBL; CP001227; ACR47513.1; -; Genomic_DNA.
DR RefSeq; YP_002916562.1; NC_012730.1.
DR STRING; 562019.RPR_04080; -.
DR EnsemblBacteria; ACR47513; ACR47513; RPR_04080.
DR GeneID; 7942617; -.
DR KEGG; rpk:RPR_04080; -.
DR PATRIC; 17899171; VBIRicPea48268_0799.
DR eggNOG; COG0571; -.
DR HOGENOM; HOG000246808; -.
DR KO; K03685; -.
DR OMA; LTHKSCK; -.
DR ProtClustDB; PRK00102; -.
DR BioCyc; RPEA562019:GJD7-519-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004525; F:ribonuclease III activity; IEA:HAMAP.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006397; P:mRNA processing; IEA:HAMAP.
DR GO; GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
DR GO; GO:0016075; P:rRNA catabolic process; IEA:InterPro.
DR GO; GO:0006364; P:rRNA processing; IEA:HAMAP.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1520.10; -; 1.
DR Gene3D; 3.30.160.20; -; 1.
DR HAMAP; MF_00104; RNase_III; 1; -.
DR InterPro; IPR001159; Ds-RNA-bd.
DR InterPro; IPR014720; dsRNA-bd-like_dom.
DR InterPro; IPR011907; RNase_III.
DR InterPro; IPR000999; RNase_III_dom.
DR PANTHER; PTHR11207; PTHR11207; 1.
DR Pfam; PF00035; dsrm; 1.
DR Pfam; PF00636; Ribonuclease_3; 1.
DR SMART; SM00358; DSRM; 1.
DR SMART; SM00535; RIBOc; 1.
DR SUPFAM; SSF69065; RNase_III; 1.
DR TIGRFAMs; TIGR02191; RNaseIII; 1.
DR PROSITE; PS50137; DS_RBD; 1.
DR PROSITE; PS00517; RNASE_3_1; 1.
DR PROSITE; PS50142; RNASE_3_2; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Endonuclease; Hydrolase; Magnesium;
KW Metal-binding; mRNA processing; Nuclease; RNA-binding;
KW rRNA processing; rRNA-binding; tRNA processing.
FT CHAIN 1 227 Ribonuclease 3.
FT /FTId=PRO_1000202845.
FT DOMAIN 4 133 RNase III.
FT DOMAIN 158 226 DRBM.
FT ACT_SITE 50 50 Potential.
FT ACT_SITE 122 122 By similarity.
FT METAL 46 46 Magnesium (By similarity).
FT METAL 119 119 Magnesium (By similarity).
FT METAL 122 122 Magnesium (By similarity).
SQ SEQUENCE 227 AA; 26051 MW; 6193611E507843BE CRC64;
MESFEKLEKL LSYSFKNKEL LIEALSHPSL RQHHEYKDDK DYERLEFLGD AVLNLVITEI
LFRNFANYNE GNLAKIRSYL VCKETICMVG AKLTLKNYII MTHGEEVAGG RDNLNNIENA
TEALIAAIYL DSNIETTHDI IEKLWAEFIK VQNLTDYDPK TALQEWAQAS DHHLPIYRLI
KREGASHAST FTVLVKVKDY EQTGTGHAIK EAEKNAARSL LHRLKND
//
