ID C4R0J3_KOMPG Unreviewed; 547 AA.
AC C4R0J3;
DT 07-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 07-JUL-2009, sequence version 1.
DT 24-JAN-2024, entry version 80.
DE RecName: Full=methionine--tRNA ligase {ECO:0000256|ARBA:ARBA00012838};
DE EC=6.1.1.10 {ECO:0000256|ARBA:ARBA00012838};
GN OrderedLocusNames=PAS_chr2-1_0396 {ECO:0000313|EMBL:CAY69017.1};
OS Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Phaffomycetaceae; Komagataella.
OX NCBI_TaxID=644223 {ECO:0000313|EMBL:CAY69017.1, ECO:0000313|Proteomes:UP000000314};
RN [1] {ECO:0000313|EMBL:CAY69017.1, ECO:0000313|Proteomes:UP000000314}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GS115 / ATCC 20864 {ECO:0000313|Proteomes:UP000000314};
RX PubMed=19465926; DOI=10.1038/nbt.1544;
RA De Schutter K., Lin Y.C., Tiels P., Van Hecke A., Glinka S.,
RA Weber-Lehmann J., Rouze P., Van de Peer Y., Callewaert N.;
RT "Genome sequence of the recombinant protein production host Pichia
RT pastoris.";
RL Nat. Biotechnol. 27:561-566(2009).
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363039}.
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DR EMBL; FN392320; CAY69017.1; -; Genomic_DNA.
DR RefSeq; XP_002491297.1; XM_002491252.1.
DR AlphaFoldDB; C4R0J3; -.
DR SMR; C4R0J3; -.
DR STRING; 644223.C4R0J3; -.
DR EnsemblFungi; CAY69017; CAY69017; PAS_chr2-1_0396.
DR GeneID; 8198864; -.
DR KEGG; ppa:PAS_chr2-1_0396; -.
DR eggNOG; KOG0436; Eukaryota.
DR HOGENOM; CLU_009710_9_0_1; -.
DR InParanoid; C4R0J3; -.
DR OMA; MDTQAFC; -.
DR OrthoDB; 166496at2759; -.
DR Proteomes; UP000000314; Chromosome 2.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004825; F:methionine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006431; P:methionyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd00814; MetRS_core; 1.
DR Gene3D; 2.170.220.10; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR InterPro; IPR041872; Anticodon_Met.
DR InterPro; IPR014758; Met-tRNA_synth.
DR InterPro; IPR023457; Met-tRNA_synth_2.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR033911; MetRS_core.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR NCBIfam; TIGR00398; metG; 1.
DR PANTHER; PTHR43326:SF1; METHIONINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43326; METHIONYL-TRNA SYNTHETASE; 1.
DR Pfam; PF19303; Anticodon_3; 1.
DR Pfam; PF09334; tRNA-synt_1g; 2.
DR PRINTS; PR01041; TRNASYNTHMET.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363039};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363039};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363039};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363039};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363039};
KW Reference proteome {ECO:0000313|Proteomes:UP000000314}.
FT DOMAIN 12..147
FT /note="Methionyl/Leucyl tRNA synthetase"
FT /evidence="ECO:0000259|Pfam:PF09334"
FT DOMAIN 154..368
FT /note="Methionyl/Leucyl tRNA synthetase"
FT /evidence="ECO:0000259|Pfam:PF09334"
FT DOMAIN 411..510
FT /note="Methionyl-tRNA synthetase anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF19303"
SQ SEQUENCE 547 AA; 62595 MW; B0F875E23033667B CRC64;
MVGAYLSSAS KYFITTPIFY VNAAPHLGHL YSMLLCDFQK RWLDLKETPN YFTTGTDEHG
LKVQIAAELN KTDPRLFCDN LSNKFKHLAQ ISDIQYDRFI RTTDADHLAT VRQFWETVWK
NGYIYKGKHS GWYSVSDETF YPESQVEQRD GKTVSTETGS EVVYESEENY FFKLSAFQSQ
LIDLYKANPQ FVIPANYYSD LLKELEREPL QDLSISRPSS RLQWGISVPN DESQKIYVWF
DALVNYLTSI GYPSSRYKEF WPGTHLIGKD ISRFHCIYWP AFLMAAKVSP PEKVVVHGHW
LMGGSKMSKS KGNVADPLAI GDYYGVDSLR LFLGANSILS NDCDFTERKL NDHRDLFIDK
ICNVVTRCIS KSFDPARSVM SFSNEDMNLV WKRILERGWE GDLEKAAELK KSYDEIVDDA
QQLFSKIDVL MSTFTTHKSV GEIFDLMLKV NQFVNSTEPW NLKSNERLQD LSIFVALDVT
RVCLILLQPF IPGLSNKLLD MIKVESSKRT ATYAAFQQDS SYGQGAKFLK GSTPPLIKVP
LRAGAYE
//
