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Database: UniProt
Entry: C4Z566
LinkDB: C4Z566
Original site: C4Z566 
ID   ILVD_EUBE2              Reviewed;         557 AA.
AC   C4Z566;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   26-NOV-2014, entry version 39.
DE   RecName: Full=Dihydroxy-acid dehydratase {ECO:0000255|HAMAP-Rule:MF_00012};
DE            Short=DAD {ECO:0000255|HAMAP-Rule:MF_00012};
DE            EC=4.2.1.9 {ECO:0000255|HAMAP-Rule:MF_00012};
GN   Name=ilvD {ECO:0000255|HAMAP-Rule:MF_00012};
GN   OrderedLocusNames=EUBELI_00762;
OS   Eubacterium eligens (strain ATCC 27750 / VPI C15-48).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Eubacteriaceae;
OC   Eubacterium.
OX   NCBI_TaxID=515620;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27750 / VPI C15-48;
RX   PubMed=19321416; DOI=10.1073/pnas.0901529106;
RA   Mahowald M.A., Rey F.E., Seedorf H., Turnbaugh P.J., Fulton R.S.,
RA   Wollam A., Shah N., Wang C., Magrini V., Wilson R.K., Cantarel B.L.,
RA   Coutinho P.M., Henrissat B., Crock L.W., Russell A., Verberkmoes N.C.,
RA   Hettich R.L., Gordon J.I.;
RT   "Characterizing a model human gut microbiota composed of members of
RT   its two dominant bacterial phyla.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:5859-5864(2009).
CC   -!- CATALYTIC ACTIVITY: 2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-
CC       oxobutanoate + H(2)O. {ECO:0000255|HAMAP-Rule:MF_00012}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00012};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000255|HAMAP-Rule:MF_00012};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-
CC       isoleucine from 2-oxobutanoate: step 3/4. {ECO:0000255|HAMAP-
CC       Rule:MF_00012}.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine
CC       from pyruvate: step 3/4. {ECO:0000255|HAMAP-Rule:MF_00012}.
CC   -!- SIMILARITY: Belongs to the IlvD/Edd family. {ECO:0000255|HAMAP-
CC       Rule:MF_00012}.
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DR   EMBL; CP001104; ACR71770.1; -; Genomic_DNA.
DR   RefSeq; WP_012739006.1; NC_012778.1.
DR   RefSeq; YP_002930217.1; NC_012778.1.
DR   STRING; 515620.EUBELI_00762; -.
DR   EnsemblBacteria; ACR71770; ACR71770; EUBELI_00762.
DR   GeneID; 7957310; -.
DR   KEGG; eel:EUBELI_00762; -.
DR   PATRIC; 21864686; VBIEubEli113401_0704.
DR   eggNOG; COG0129; -.
DR   HOGENOM; HOG000173155; -.
DR   KO; K01687; -.
DR   OMA; ELYEIEC; -.
DR   OrthoDB; EOG6MSS24; -.
DR   BioCyc; EELI515620:GH1N-762-MONOMER; -.
DR   UniPathway; UPA00047; UER00057.
DR   UniPathway; UPA00049; UER00061.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0004160; F:dihydroxy-acid dehydratase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-HAMAP.
DR   GO; GO:0009099; P:valine biosynthetic process; IEA:UniProtKB-HAMAP.
DR   HAMAP; MF_00012; IlvD; 1.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR004404; DihydroxyA_deHydtase.
DR   InterPro; IPR000581; DiOHA_6PGluconate_deHydtase.
DR   InterPro; IPR020558; DiOHA_6PGluconate_deHydtase_CS.
DR   PANTHER; PTHR21000; PTHR21000; 1.
DR   Pfam; PF00920; ILVD_EDD; 1.
DR   SUPFAM; SSF52016; SSF52016; 1.
DR   TIGRFAMs; TIGR00110; ilvD; 1.
DR   PROSITE; PS00886; ILVD_EDD_1; 1.
DR   PROSITE; PS00887; ILVD_EDD_2; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Amino-acid biosynthesis;
KW   Branched-chain amino acid biosynthesis; Complete proteome; Iron;
KW   Iron-sulfur; Lyase; Metal-binding; Reference proteome.
FT   CHAIN         1    557       Dihydroxy-acid dehydratase.
FT                                /FTId=PRO_1000201777.
FT   METAL       119    119       Iron-sulfur (4Fe-4S). {ECO:0000255|HAMAP-
FT                                Rule:MF_00012}.
FT   METAL       191    191       Iron-sulfur (4Fe-4S). {ECO:0000255|HAMAP-
FT                                Rule:MF_00012}.
SQ   SEQUENCE   557 AA;  58342 MW;  1CE38E442EE6ECE0 CRC64;
     MISDNARSGM QQAPARSLFN ALGFTAEEMK KPMIGIVSSY NEIVPGHMNI DKIVNAVKLG
     VAEAGGVPVV FPAIAVCDGI AMGHVGMKYS LVTRDLIADS TECMAIAHQF DGLVMVPNCD
     KNVPGLLMAA ARLNLPTVFV SGGPMLAGHV KGKKRSLSSM FEAVGSYAAG TMTEEDVLEF
     EEKVCPTCGS CSGMYTANSM NCLTEALGMG LRGNGTIPAV YSERIKLAKH AGMAVMDMVN
     KGITARDIIT KDSIMNALTV DMALGCSTNS MLHLPAIAHE IGFDFDIKFA NPISEKTPNL
     CHLAPAGPTY MEDLNEAGGV YAVMKELADI GLLNTDCLTV SGKTIGECIA TAYNRDPEVI
     RTVDNAYSKT GGLAVLSGNL APDGSVVKRS AVVPEMLVHE GPARVFDSEE DAIAAIKGGK
     IVEGDVVVIR YEGPKGGPGM REMLNPTSAI AGMGLGSSVA LITDGRFSGA SRGASIGHVS
     PEAAVGGPIA LVEEGDIISI DIPGLKLELK VSDEELAARK AKWQPREPKV TTGYLKRYAS
     LVTSGNRGAI LKSSADE
//
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