ID C5A2Z3_THEGJ Unreviewed; 919 AA.
AC C5A2Z3;
DT 28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 28-JUL-2009, sequence version 1.
DT 27-MAR-2024, entry version 83.
DE RecName: Full=UDP-N-acetyl-D-mannosamine dehydrogenase {ECO:0000256|ARBA:ARBA00016796};
DE EC=1.1.1.336 {ECO:0000256|ARBA:ARBA00012935};
DE AltName: Full=UDP-ManNAc 6-dehydrogenase {ECO:0000256|ARBA:ARBA00030172};
GN OrderedLocusNames=TGAM_2152 {ECO:0000313|EMBL:ACS34654.1};
OS Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Thermococcus.
OX NCBI_TaxID=593117 {ECO:0000313|EMBL:ACS34654.1, ECO:0000313|Proteomes:UP000001488};
RN [1] {ECO:0000313|EMBL:ACS34654.1, ECO:0000313|Proteomes:UP000001488}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15229 / JCM 11827 / EJ3
RC {ECO:0000313|Proteomes:UP000001488};
RX PubMed=19558674; DOI=10.1186/gb-2009-10-6-r70;
RA Zivanovic Y., Armengaud J., Lagorce A., Leplat C., Guerin P., Dutertre M.,
RA Anthouard V., Forterre P., Wincker P., Confalonieri F.;
RT "Genome analysis and genome-wide proteomics of Thermococcus gammatolerans,
RT the most radioresistant organism known amongst the Archaea.";
RL Genome Biol. 10:R70.1-R70.23(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + 2 NAD(+) + UDP-N-acetyl-alpha-D-mannosamine = 3 H(+) + 2
CC NADH + UDP-N-acetyl-alpha-D-mannosaminouronate; Xref=Rhea:RHEA:25780,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:68623, ChEBI:CHEBI:70731;
CC EC=1.1.1.336; Evidence={ECO:0000256|ARBA:ARBA00001205};
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DR EMBL; CP001398; ACS34654.1; -; Genomic_DNA.
DR AlphaFoldDB; C5A2Z3; -.
DR STRING; 593117.TGAM_2152; -.
DR MEROPS; N10.005; -.
DR PaxDb; 593117-TGAM_2152; -.
DR KEGG; tga:TGAM_2152; -.
DR PATRIC; fig|593117.10.peg.2161; -.
DR eggNOG; arCOG00252; Archaea.
DR HOGENOM; CLU_317051_0_0_2; -.
DR OrthoDB; 372050at2157; -.
DR Proteomes; UP000001488; Chromosome.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0016628; F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0089714; F:UDP-N-acetyl-D-mannosamine dehydrogenase activity; IEA:RHEA.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR GO; GO:0000271; P:polysaccharide biosynthetic process; IEA:InterPro.
DR CDD; cd00081; Hint; 1.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 2.
DR Gene3D; 3.10.28.10; Homing endonucleases; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR017476; UDP-Glc/GDP-Man.
DR InterPro; IPR014027; UDP-Glc/GDP-Man_DH_C.
DR InterPro; IPR036220; UDP-Glc/GDP-Man_DH_C_sf.
DR InterPro; IPR014026; UDP-Glc/GDP-Man_DH_dimer.
DR InterPro; IPR001732; UDP-Glc/GDP-Man_DH_N.
DR InterPro; IPR028359; UDP_ManNAc/GlcNAc_DH.
DR NCBIfam; TIGR01443; intein_Cterm; 1.
DR NCBIfam; TIGR01445; intein_Nterm; 1.
DR NCBIfam; TIGR03026; NDP-sugDHase; 1.
DR PANTHER; PTHR43491; UDP-N-ACETYL-D-MANNOSAMINE DEHYDROGENASE; 1.
DR PANTHER; PTHR43491:SF5; UDP-N-ACETYL-D-MANNOSAMINE DEHYDROGENASE; 1.
DR Pfam; PF14890; Intein_splicing; 1.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR Pfam; PF00984; UDPG_MGDP_dh; 1.
DR Pfam; PF03720; UDPG_MGDP_dh_C; 1.
DR Pfam; PF03721; UDPG_MGDP_dh_N; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SMART; SM00984; UDPG_MGDP_dh_C; 1.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 1.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR SUPFAM; SSF55608; Homing endonucleases; 2.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF52413; UDP-glucose/GDP-mannose dehydrogenase C-terminal domain; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 4: Predicted;
KW Autocatalytic cleavage {ECO:0000256|ARBA:ARBA00022813};
KW Endonuclease {ECO:0000313|EMBL:ACS34654.1};
KW Hydrolase {ECO:0000313|EMBL:ACS34654.1};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Nuclease {ECO:0000313|EMBL:ACS34654.1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000313|EMBL:ACS34654.1};
KW Protein splicing {ECO:0000256|ARBA:ARBA00023000}.
FT DOMAIN 499..651
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000259|PROSITE:PS50819"
FT DOMAIN 725..748
FT /note="Intein C-terminal splicing"
FT /evidence="ECO:0000259|PROSITE:PS50818"
SQ SEQUENCE 919 AA; 102534 MW; 5EB01695F7C889FC CRC64;
MKLIGLNKEE VKRVLKEGKV TIAVYGLGKM GLPLAAVFAD HGANVIGVDI NEKVVEMINR
GENHVKEEPG LDELVRRNVE AGRLKATTDG VWAAKQADVM VILVPTLTDE RGNLKLGPVY
DVAEKISQGL ERGDIVITEA TMPPGTTESL IPILEKSGLK LGEFGLAHAP ERTMTGTAIR
DITGQYPKIV GASDEKTLEA VIGIYETINR KGVIPMSSIK AAEAVKVFEG VYRDVNIALA
NELAMWCEEH GLDALEVFQA ANTQPYCHLH MPGAGVGGHC FAKDEFVFLE EGGIEPLSVE
ELFELIEGKT EEIDGVLVKH PNEEYRILSF DPVSRRPVFG RVTLATKRRL KPREKVLEIK
TKTGRSVKLT ENHIVWVYKD GAFVEKLAKD LTPGDMMTIL TELPSKETPE VIDVISILSR
DPQGYRIKFQ GIPAESRAAL HAKLAPKSYD YTKGNYIPLE YYSEFKNLLP RPQAIATGRG
PSYLEFPPEI HVNEEFATLL GYYISEGCIT EDSSLRTRFS FNIRETDYIG EVRGILENIG
IRHSEYVQGS VHHIKVSSRA FGELLRFLGT GVNSYDAKVP GFILYSKALQ RAVLRGIING
DGSVSVYEGK HRTKKNGREY ITEKISASVE VATASNKLAQ QLFLMLQNLG IVPSYKRNGN
VHILRIFGPE NLEKLRGIVS GEKGEKLEYY LSNIKKRERN SSFKPMGSFA LVEVKEIKPI
EYDDYVYSFE VEGTNTIVTT SGLIVHNCIP VYPWFVINLA RKTNPRLTKT AREINDSMPH
HVVELTVKAL NEVGRPVKGS NILVLGLTFR GGVREFMKAA AKPIIEELKE WGANVYAYDP
LCTREDAERF GAEWKEDFRD IDAIVITADH KEFRELDLEK LAKEVRTRVI VDGRNVLDPA
EAEEKGFVYL RVGRTAYSP
//
