ID C5CT62_VARPS Unreviewed; 809 AA.
AC C5CT62;
DT 28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 28-JUL-2009, sequence version 1.
DT 27-MAR-2024, entry version 77.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN OrderedLocusNames=Vapar_1536 {ECO:0000313|EMBL:ACS18187.1};
OS Variovorax paradoxus (strain S110).
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Variovorax.
OX NCBI_TaxID=543728 {ECO:0000313|EMBL:ACS18187.1};
RN [1] {ECO:0000313|EMBL:ACS18187.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S110 {ECO:0000313|EMBL:ACS18187.1};
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D.,
RA Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Orwin P.,
RA Leadbetter J.R., Spain J.C., Han J.I.;
RT "Complete sequence of chromosome 1 of Variovorax paradoxus S110.";
RL Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP001635; ACS18187.1; -; Genomic_DNA.
DR AlphaFoldDB; C5CT62; -.
DR STRING; 543728.Vapar_1536; -.
DR KEGG; vap:Vapar_1536; -.
DR eggNOG; COG0210; Bacteria.
DR HOGENOM; CLU_004585_5_2_4; -.
DR OrthoDB; 5905204at2; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 25..313
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 314..622
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 738..759
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 46..53
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 809 AA; 89629 MW; 844B30068B189131 CRC64;
MSLFGSYDAT AGAPPSPAAD SPLLANLNPE QRAAVTLPAG NALILAGAGS GKTRVLTTRI
AWLLQTGQVS PGGILAVTFT NKAAKEMMTR LTAMLPVNVR GMWIGTFHGL CNRFLRAHWK
LANLPSTFQI LDTQDQLSAI KRLMKQFNVD DERFPAKQTQ WFIAGAKEDG LRPKDVEIRT
EDDRKKVELY QLYEEQCQRE GVVDFGELML RSYELLRDND PVREHYQRRF RHILIDEFQD
TNRLQYAWIK MLSGSTAAGG QFVPGENNSV IAVGDDDQSI YAFRGARVGN MADFVREFDV
RHQIKLEQNY RSYSNILDSA NELISHNKKR LGKNLRTDQG PGEPVRVYES PTDLAEAQWM
VEEMRQLVRD GFDRKEMAVL YRSNAQSRVI ETALFNASVP YRVYGGLRFF ERAEIKHALA
YLRLLENKDD DTSFLRVVNF PPRGIGARTL EQLQDAARTA GRSLHDAVHA VGGKAGSNLT
AFVAKIDVLR EQTQGASLRE IIELVLDHSG LVEHYKADRE GADRIENLEE LVNAAESFVT
QEGFGRDAVA LPIDELRQSP ASQGLDPNRP VIDEPLAPDA ETGETLSPLA AFLTHAALES
GDNQAQAGQD AVQLMTVHAA KGLEFDCVFI TGMEEGLFPH ENSMSDFESL EEERRLMYVA
ITRARKRLYL SHSQTRMLHG QTRYNVKSRF FEELPEGALK WLTPKNQGFT PSAFGYGGGY
ASTRGGGGSY SSGGGYGGSK DTFASPPVPP QKAAPSHGLR SGMQVFHNKF GEGTVLSLEG
SGDDARAQVK FARHGVKWLA LSVAKLTPV
//
