ID C5H029_9VIBR Unreviewed; 308 AA.
AC C5H029;
DT 28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 28-JUL-2009, sequence version 1.
DT 13-SEP-2023, entry version 47.
DE RecName: Full=DNA-directed RNA polymerase subunit alpha {ECO:0000256|ARBA:ARBA00015972};
DE EC=2.7.7.6 {ECO:0000256|ARBA:ARBA00012418};
DE AltName: Full=RNA polymerase subunit alpha {ECO:0000256|ARBA:ARBA00033070};
DE AltName: Full=Transcriptase subunit alpha {ECO:0000256|ARBA:ARBA00032524};
DE Flags: Fragment;
GN Name=rpoA {ECO:0000313|EMBL:ABY62520.1};
OS Vibrio sp. cann.2.3.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=491943 {ECO:0000313|EMBL:ABY62520.1};
RN [1] {ECO:0000313|EMBL:ABY62520.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Cann.2.3 {ECO:0000313|EMBL:ABY62520.1};
RX PubMed=19481895; DOI=10.1016/j.syapm.2009.04.005;
RA Ast J.C., Urbanczyk H., Dunlap P.V.;
RT "Multi-gene analysis reveals previously unrecognized phylogenetic diversity
RT in Aliivibrio.";
RL Syst. Appl. Microbiol. 32:379-386(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550};
CC -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC {ECO:0000256|ARBA:ARBA00007123}.
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DR EMBL; EU185967; ABY62520.1; -; Genomic_DNA.
DR AlphaFoldDB; C5H029; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd06928; RNAP_alpha_NTD; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 2.170.120.12; DNA-directed RNA polymerase, insert domain; 1.
DR Gene3D; 3.30.1360.10; RNA polymerase, RBP11-like subunit; 1.
DR HAMAP; MF_00059; RNApol_bact_RpoA; 1.
DR InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR InterPro; IPR011773; DNA-dir_RpoA.
DR InterPro; IPR036603; RBP11-like.
DR InterPro; IPR011260; RNAP_asu_C.
DR InterPro; IPR036643; RNApol_insert_sf.
DR NCBIfam; TIGR02027; rpoA; 1.
DR Pfam; PF01000; RNA_pol_A_bac; 1.
DR Pfam; PF03118; RNA_pol_A_CTD; 1.
DR Pfam; PF01193; RNA_pol_L; 1.
DR SMART; SM00662; RPOLD; 1.
DR SUPFAM; SSF47789; C-terminal domain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF56553; Insert subdomain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF55257; RBP11-like subunits of RNA polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000313|EMBL:ABY62520.1};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 16..227
FT /note="DNA-directed RNA polymerase RpoA/D/Rpb3-type"
FT /evidence="ECO:0000259|SMART:SM00662"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ABY62520.1"
FT NON_TER 308
FT /evidence="ECO:0000313|EMBL:ABY62520.1"
SQ SEQUENCE 308 AA; 34072 MW; 3E766BB2F45505AE CRC64;
FLKPRLVDIE QVSQTHAKVT LEPLERGFGH TLGNALRRIL LSSMPGCAVT EVEIEGVLHE
YSTKEGVQED ILEILLNLKG LAVKVEGKDE VIITLNKSGA GPVVAGDITH DGDVEIANPE
HVICHLTDDN AEISMRIKVE RGRGYVPSTA RIHTEEDERP IGRLLIDATY SPVDKISYSV
EAARVEQRTD LDKLVIDMET NGTLDPEEAI RRAATILAEQ LDAFVDLRDV RVPEEKEEKP
EFDPILLRPV DDLELTVRSA NCLKAEAIHY IGDLVQRTEV ELLKTPNLGK KSLTEIKDVL
ASRGLSLG
//
