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Database: UniProt
Entry: C6E557
LinkDB: C6E557
Original site: C6E557 
ID   TRMFO_GEOSM             Reviewed;         435 AA.
AC   C6E557;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-SEP-2009, sequence version 1.
DT   26-NOV-2014, entry version 36.
DE   RecName: Full=Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO {ECO:0000255|HAMAP-Rule:MF_01037};
DE            EC=2.1.1.74 {ECO:0000255|HAMAP-Rule:MF_01037};
DE   AltName: Full=Folate-dependent tRNA (uracil-5-)-methyltransferase {ECO:0000255|HAMAP-Rule:MF_01037};
DE   AltName: Full=Folate-dependent tRNA(M-5-U54)-methyltransferase {ECO:0000255|HAMAP-Rule:MF_01037};
GN   Name=trmFO {ECO:0000255|HAMAP-Rule:MF_01037};
GN   OrderedLocusNames=GM21_1530;
OS   Geobacter sp. (strain M21).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales;
OC   Geobacteraceae; Geobacter.
OX   NCBI_TaxID=443144;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=M21;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T.,
RA   Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Ovchinnikova G., Lovley D.;
RT   "Complete sequence of Geobacter sp. M21.";
RL   Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the folate-dependent formation of 5-methyl-
CC       uridine at position 54 (M-5-U54) in all tRNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_01037}.
CC   -!- CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + uracil(54) in
CC       tRNA + FADH(2) = tetrahydrofolate + 5-methyluracil(54) in tRNA +
CC       FAD. {ECO:0000255|HAMAP-Rule:MF_01037}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01037};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01037}.
CC   -!- SIMILARITY: Belongs to the MnmG family. TrmFO subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01037}.
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DR   EMBL; CP001661; ACT17586.1; -; Genomic_DNA.
DR   RefSeq; WP_015836836.1; NC_012918.1.
DR   RefSeq; YP_003021344.1; NC_012918.1.
DR   ProteinModelPortal; C6E557; -.
DR   STRING; 443144.GM21_1530; -.
DR   EnsemblBacteria; ACT17586; ACT17586; GM21_1530.
DR   GeneID; 8136859; -.
DR   KEGG; gem:GM21_1530; -.
DR   PATRIC; 22017506; VBIGeoSp56140_1487.
DR   eggNOG; COG1206; -.
DR   HOGENOM; HOG000252054; -.
DR   KO; K04094; -.
DR   OMA; EIYFEGC; -.
DR   OrthoDB; EOG6J74VT; -.
DR   BioCyc; GSP443144:GHKM-1560-MONOMER; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-HAMAP.
DR   GO; GO:0030698; F:5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0047151; F:methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity; IEA:UniProtKB-EC.
DR   HAMAP; MF_01037; TrmFO; 1.
DR   InterPro; IPR004417; Folate-dep_Ribothymidyl_synth.
DR   InterPro; IPR002218; GIDA-rel.
DR   Pfam; PF01134; GIDA; 1.
DR   TIGRFAMs; TIGR00137; gid_trmFO; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; FAD; Flavoprotein; Methyltransferase;
KW   Transferase; tRNA processing.
FT   CHAIN         1    435       Methylenetetrahydrofolate--tRNA-(uracil-
FT                                5-)-methyltransferase TrmFO.
FT                                /FTId=PRO_1000213383.
FT   NP_BIND       9     14       FAD. {ECO:0000255|HAMAP-Rule:MF_01037}.
SQ   SEQUENCE   435 AA;  47749 MW;  1DC9DF29DEFE9969 CRC64;
     MTQQITVIGG GLAGCEAAWQ AAKRGVKVRL FEMKPNCYSE AHHLPGLSEL VCSNSLRGDS
     LENAVGLLKE ELRRLESLFM EGAEATKVPA GGALAVDRDL FSQYITSRIE SHPLIEVVRE
     EVTRIPEEGI VVLASGPLTA GLLAQEIGRL AGSYLYFYDA IAPIVAADSI DYGKAFRASR
     YGKGDGDDYV NCPMDEEQYQ AFVREILAAE KVEPKSFEKV VHFEGCMPIE EMASRGPETL
     RFGPMKPVGL VDPRVGVEPH AVIQLRQENL EATMYNLVGF QTKLTWPEQK RIFRMIPGLE
     NAQFLRLGSM HRNTFINAPE LLMATCQLKS DQRIFFAGQI TGVEGYVESA SSGFAVGVNA
     ARLSKGEGLV VPPAETAIGA LARHITNTEA AHFQPMNVNY GLFPPLPGRI KKKEKRGLLA
     QRGLEALEMW LPELS
//
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