ID C6LP76_GIAIB Unreviewed; 1742 AA.
AC C6LP76;
DT 22-SEP-2009, integrated into UniProtKB/TrEMBL.
DT 22-SEP-2009, sequence version 1.
DT 27-MAR-2024, entry version 61.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN ORFNames=GL50581_540 {ECO:0000313|EMBL:EET02185.1};
OS Giardia intestinalis (strain ATCC 50581 / GS clone H7) (Giardia lamblia).
OC Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX NCBI_TaxID=598745 {ECO:0000313|EMBL:EET02185.1, ECO:0000313|Proteomes:UP000002488};
RN [1] {ECO:0000313|EMBL:EET02185.1, ECO:0000313|Proteomes:UP000002488}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 50581 / GS clone H7 {ECO:0000313|Proteomes:UP000002488};
RX PubMed=19696920; DOI=10.1371/journal.ppat.1000560;
RA Franzen O., Jerlstrom-Hultqvist J., Castro E., Sherwood E., Ankarklev J.,
RA Reiner D.S., Palm D., Andersson J.O., Andersson B., Svard S.G.;
RT "Draft genome sequencing of giardia intestinalis assemblage B isolate GS:
RT is human giardiasis caused by two different species?";
RL PLoS Pathog. 5:E1000560-E1000560(2009).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|RuleBase:RU004279};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EET02185.1}.
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DR EMBL; ACGJ01000686; EET02185.1; -; Genomic_DNA.
DR EnsemblProtists; EET02185; EET02185; GL50581_540.
DR VEuPathDB; GiardiaDB:GL50581_540; -.
DR OMA; AVCPPYN; -.
DR OrthoDB; 169836at2759; -.
DR Proteomes; UP000002488; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd02736; RNAP_III_Rpc1_C; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 2.40.40.20; -; 2.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 6.20.50.80; -; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR035698; RNAP_III_Rpc1_C.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF32; DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 2.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 2.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 304..776
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 576..619
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1720..1742
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 576..602
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 603..619
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1742 AA; 194057 MW; E4C3D1F5436AA2A6 CRC64;
MDPSRRLRHF RSEIPQLVTE HRFPKRVEKL VFSVLSSNAL MKLSEVQITT PILYEHYPSP
ARNGVLDKRL GLSDKVGRCA TCGKDTKMCI GHFGTIALAL PVFHPGLIND ARTMLSIICP
FCSRVKISEA DRHKYKSLNH KHSLSIVKEA QEQAKKQVNC PFCHAPSTVC KKSQGFRVLR
EMHYEESATK ILAGVIKHQK EIEKIQDLDQ RLRNHVVDPI QALHILKKVP ECDYPYLGIP
WQSRNVYASQ QTPTSIASND ASQLFSRGSS KLLARTANRF IAQHQETGEH VTLLRSQHFL
TRPQDVILTH IPVPPSCLRP SVQSGSGAGT TEDSLTSHLV RILTVNDKLK EAVTSGAVTT
SKVYAKWDQL NMDVSLLYIG KIPGGVQPVK GKIDSGTKEG TGIIDRLKGK AGRFRSNLSG
KRVNFSGRTV ISPNPYLSMQ QVAIPRLIAQ NLTFPEIVTS RNIRFLRELI LNGKTYPGAN
EVLLLNDTIR YNPALKRLGI DMKRSRDMEA KAMELVSRCV DTFNLNSLCA DLPELQAAIQ
HLVSESSNAI NSEVKTESLP QESTLFSKDV HDPITRDDLD SVVDDTSETN EPKLEDIHAN
RTDESAGQLQ TNTVDPSSEP KTVLKLNSIA SLQLTNASSQ HLSTFKLNTV SLLQKDRRGR
QAIANSLRPN DIVYRHLLPN DTLLFNRQPS LHRMSIMQFN AVIHENRTFS FNPIVCSPFN
ADFDGDEMNI FYMQGQEARA EAGILMGSHE NIISPRHGEC MIGLTQDFLT GIYLLSGKGI
FMTRQDYCQH VSYGCDGFGD ATYGVSLYNY GREFIKSIHS RRGTEEDTVG FVKVPCLAQP
CIVYPRQLYS GKQVLSVLMK GNDFDSVSIN LEHGDKTYKK SDDKRALSVG DDYVVIQNSE
HLAGRLTKAF LASSKNCIFY FLVQNYGPVS AARIMLRFAK VAARFLMNYG FTIGIDDVMP
SQQVLHKKEL IVQEGYERAQ EKIHDYESGK LHAIPGSTVQ ETLEAALNQI LSNVRESCAQ
VALKELHFTN KPLIMSLCGS KGSPINIAQM IIILGQQSFG GSRAPDDFYT RSAPYFYHYS
KEPNSKGFIL NSFYTGLLPF EFLAHARAGR DGVIDSACKT ADTGYLQRRL VKCLEDLSVS
YDFTVRNSKK NVVQFRFGHD GFDPIKLEVG TAQCLDLDTL LRHIILLNRS QDLAEGVPLV
LLDRYKAEAE LETLLNKDFY GQEACQIERD NVISYFRSNI LPKITANTRI AAEIAPALAA
SFLQTTITRK DIDDFLRQVR RKYIRLNLEP GSPCGAVAAQ SVGEPSTQMT LKSFHHAGLA
SMNITQGVPR LKEIVDGVVK ISTPITTVEL HIDADEEEDP AIITEKYLEK ARMMKNIIEC
TYLGQISASI IECYSQSSCH IEVNLDMKII ADMGLAGVIT VETVVASILH NDKAKKLVGQ
DHSSGSVSIH SGTRFSVRPI TQSRENLLFD IQSLKLLLPM IPVSGIATCS RAVINEYKEI
TQGGTISSAP LTKYNLLVEG VGLQSILNVS GIDFTRTLSN NIIEVANTLG IEAAVATIAN
EIKACMDSHG MAVDMRHIRL LADIMCFKGK VLGFTRFGLT KMKADSVIML ASFEKTGEHL
FNAALGNKID ETNGVTESII LGKPMSMGTG SFSLLQAPYF DEKTGKTIEY QPKPVTRFLG
EVLNRQYDEE VDAIVSAFWY DEKVYLTGAE ARAKLLRERK HTNKRSWSQH REKFANSKSK
RQ
//
