ID C6WLI7_ACTMD Unreviewed; 391 AA.
AC C6WLI7;
DT 22-SEP-2009, integrated into UniProtKB/TrEMBL.
DT 22-SEP-2009, sequence version 1.
DT 27-MAR-2024, entry version 68.
DE SubName: Full=Radical SAM domain protein {ECO:0000313|EMBL:ACU38380.1};
GN OrderedLocusNames=Amir_4537 {ECO:0000313|EMBL:ACU38380.1};
OS Actinosynnema mirum (strain ATCC 29888 / DSM 43827 / JCM 3225 / NBRC 14064
OS / NCIMB 13271 / NRRL B-12336 / IMRU 3971 / 101).
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Actinosynnema.
OX NCBI_TaxID=446462 {ECO:0000313|EMBL:ACU38380.1, ECO:0000313|Proteomes:UP000002213};
RN [1] {ECO:0000313|EMBL:ACU38380.1, ECO:0000313|Proteomes:UP000002213}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29888 / DSM 43827 / JCM 3225 / NBRC 14064 / NCIMB 13271 /
RC NRRL B-12336 / IMRU 3971 / 101 {ECO:0000313|Proteomes:UP000002213};
RX PubMed=21304636; DOI=10.4056/sigs.21137;
RA Land M., Lapidus A., Mayilraj S., Chen F., Copeland A., Del Rio T.G.,
RA Nolan M., Lucas S., Tice H., Cheng J.F., Chertkov O., Bruce D., Goodwin L.,
RA Pitluck S., Rohde M., Goker M., Pati A., Ivanova N., Mavromatis K.,
RA Chen A., Palaniappan K., Hauser L., Chang Y.J., Jeffries C.C., Brettin T.,
RA Detter J.C., Han C., Chain P., Tindall B.J., Bristow J., Eisen J.A.,
RA Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.;
RT "Complete genome sequence of Actinosynnema mirum type strain (101).";
RL Stand. Genomic Sci. 1:46-53(2009).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR EMBL; CP001630; ACU38380.1; -; Genomic_DNA.
DR RefSeq; WP_015803267.1; NC_013093.1.
DR AlphaFoldDB; C6WLI7; -.
DR STRING; 446462.Amir_4537; -.
DR KEGG; ami:Amir_4537; -.
DR eggNOG; COG0535; Bacteria.
DR HOGENOM; CLU_009273_4_2_11; -.
DR OrthoDB; 9782387at2; -.
DR Proteomes; UP000002213; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd21109; SPASM; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR023885; 4Fe4S-binding_SPASM_dom.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR034391; Cmo-like_SPASM_containing.
DR InterPro; IPR007197; rSAM.
DR PANTHER; PTHR11228:SF7; PQQA PEPTIDE CYCLASE; 1.
DR PANTHER; PTHR11228; RADICAL SAM DOMAIN PROTEIN; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF13186; SPASM; 1.
DR SFLD; SFLDG01387; BtrN-like_SPASM_domain_contain; 1.
DR SFLD; SFLDG01386; main_SPASM_domain-containing; 1.
DR SFLD; SFLDG01067; SPASM/twitch_domain_containing; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000002213};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 37..270
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 391 AA; 44564 MW; 5FD525CB5D8C5CDA CRC64;
MHPRTARTLN QKEFHDLRRT VAIRAETRRN VEVDATYATP LPREVSLQLT YKCNLRCNHC
YQWNDQGFFH DFSKVKQRTE LDLDIVDDVL RTTDDTRAKV FLWGGEPLMH SRFGEIAKLL
ARTPRTVNLC TNALLIKRNL EHLLTIGPGL NVLVSLDGLG ADHEALRGKG TFPRTIDNVR
LLLDLQKSGE YQGEVSLSCM VSNETVGSMR EFMEWAEELG VNSVYFQFPW FISPEVASAM
DDMYAENFAW LNPPAPGTTP TWHSYTYRIE PGLIGALRRS MAELAERTWN VRIRYQPQLE
PDEVEDFVLG ASRPAQKRNR CLAVSNRLEV HADGKVSSCK FFPEFVIGDL HESGVAQVWQ
GEAFRRVRTV LRGTSLMPVC SKCILLYLNG V
//
