ID C7C2U7_STAA5 Unreviewed; 163 AA.
AC C7C2U7;
DT 22-SEP-2009, integrated into UniProtKB/TrEMBL.
DT 22-SEP-2009, sequence version 1.
DT 27-MAR-2024, entry version 76.
DE RecName: Full=Dihydrofolate reductase {ECO:0000256|ARBA:ARBA00012856, ECO:0000256|PIRNR:PIRNR000194};
DE EC=1.5.1.3 {ECO:0000256|ARBA:ARBA00012856, ECO:0000256|PIRNR:PIRNR000194};
GN Name=dfrK {ECO:0000313|EMBL:CAY33090.1};
OS Staphylococcus aureus (strain MRSA ST398 / isolate S0385).
OG Plasmid pKKS25 {ECO:0000313|EMBL:CAY48683.1},
OG Plasmid pKKS627 {ECO:0000313|EMBL:CAY48701.1},
OG Plasmid pKKS825 {ECO:0000313|EMBL:CAY33090.1}, and
OG Plasmid pUR2940 {ECO:0000313|EMBL:CCQ44035.1}.
OC Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=523796 {ECO:0000313|EMBL:CAY33090.1};
RN [1] {ECO:0000313|EMBL:CAY33090.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ST398 {ECO:0000313|EMBL:CAY33090.1};
RC PLASMID=pKKS825 {ECO:0000313|EMBL:CAY33090.1};
RX PubMed=19470508; DOI=10.1128/AAC.00570-09;
RA Kadlec K., Schwarz S.;
RT "Novel ABC transporter gene, vga(C), located on a multiresistance plasmid
RT from a porcine methicillin-resistant Staphylococcus aureus ST398 strain.";
RL Antimicrob. Agents Chemother. 53:3589-3591(2009).
RN [2] {ECO:0000313|EMBL:CAY48683.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=MRSA 25 {ECO:0000313|EMBL:CAY48683.1};
RC PLASMID=pKKS25 {ECO:0000313|EMBL:CAY48683.1};
RX PubMed=20008780; DOI=10.1128/AAC.01091-09;
RA Kadlec K., Schwarz S.;
RT "Identification of a plasmid-borne resistance gene cluster comprising the
RT resistance genes erm(T), dfrK, and tet(L) in a porcine methicillin-
RT resistant Staphylococcus aureus ST398 strain.";
RL Antimicrob. Agents Chemother. 54:915-918(2010).
RN [3] {ECO:0000313|EMBL:CAY33090.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=627 {ECO:0000313|EMBL:CAY48701.1}, MRSA 25
RC {ECO:0000313|EMBL:CAY48683.1}, and ST398
RC {ECO:0000313|EMBL:CAY33090.1};
RC PLASMID=pKKS25 {ECO:0000313|EMBL:CAY48683.1}, pKKS627
RC {ECO:0000313|EMBL:CAY48701.1}, pKKS825 {ECO:0000313|EMBL:CAY33090.1},
RC and pUR2940 {ECO:0000313|EMBL:CCQ44035.1};
RA Schwarz S.P.;
RL Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:CAY48701.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=627 {ECO:0000313|EMBL:CAY48701.1};
RC PLASMID=pKKS627 {ECO:0000313|EMBL:CAY48701.1};
RX PubMed=21718151; DOI=10.1089/mdr.2011.0073;
RA Lopez M., Kadlec K., Schwarz S., Torres C.;
RT "First detection of the staphylococcal trimethoprim resistance gene dfrK
RT and the dfrK-carrying transposon Tn559 in enterococci.";
RL Microb. Drug Resist. 18:13-18(2012).
RN [5] {ECO:0000313|EMBL:CCQ44035.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ST398 {ECO:0000313|EMBL:CCQ44035.1};
RC PLASMID=pUR2940 {ECO:0000313|EMBL:CCQ44035.1};
RX PubMed=23629701; DOI=10.1128/AAC.00171-13;
RA Gomez-Sanz E, Kadlec K, Fessler AT, Zarazaga M, Torres C, Schwarz S.;
RT "Novel erm(T)-carrying multiresistance plasmids from porcine and human
RT isolates of methicillin-resistant Staphylococcus aureus ST398 that also
RT harbor cadmium and copper resistance determinants.";
RL Antimicrob. Agents Chemother. 57:3275-3282(2013).
CC -!- FUNCTION: Key enzyme in folate metabolism. Catalyzes an essential
CC reaction for de novo glycine and purine synthesis, and for DNA
CC precursor synthesis. {ECO:0000256|PIRNR:PIRNR000194}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate +
CC H(+) + NADPH; Xref=Rhea:RHEA:15009, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57451, ChEBI:CHEBI:57453, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.5.1.3;
CC Evidence={ECO:0000256|PIRNR:PIRNR000194};
CC -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-
CC tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
CC {ECO:0000256|ARBA:ARBA00004903, ECO:0000256|PIRNR:PIRNR000194}.
CC -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC {ECO:0000256|ARBA:ARBA00009539, ECO:0000256|PIRNR:PIRNR000194,
CC ECO:0000256|RuleBase:RU004474}.
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DR EMBL; FN377602; CAY33090.1; -; Genomic_DNA.
DR EMBL; FN390947; CAY48683.1; -; Genomic_DNA.
DR EMBL; FN390948; CAY48701.1; -; Genomic_DNA.
DR EMBL; HF583292; CCQ44035.1; -; Genomic_DNA.
DR RefSeq; WP_012779617.1; NG_047759.1.
DR RefSeq; YP_003084336.1; NC_013034.2.
DR RefSeq; YP_003644922.1; NC_014156.1.
DR AlphaFoldDB; C7C2U7; -.
DR SMR; C7C2U7; -.
DR BindingDB; C7C2U7; -.
DR PATRIC; fig|523796.5.peg.2716; -.
DR UniPathway; UPA00077; UER00158.
DR GO; GO:0004146; F:dihydrofolate reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0046654; P:tetrahydrofolate biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00209; DHFR; 1.
DR Gene3D; 3.40.430.10; Dihydrofolate Reductase, subunit A; 1.
DR InterPro; IPR012259; DHFR.
DR InterPro; IPR024072; DHFR-like_dom_sf.
DR InterPro; IPR017925; DHFR_CS.
DR InterPro; IPR001796; DHFR_dom.
DR PANTHER; PTHR48069; DIHYDROFOLATE REDUCTASE; 1.
DR PANTHER; PTHR48069:SF3; DIHYDROFOLATE REDUCTASE; 1.
DR Pfam; PF00186; DHFR_1; 1.
DR PIRSF; PIRSF000194; DHFR; 1.
DR PRINTS; PR00070; DHFR.
DR SUPFAM; SSF53597; Dihydrofolate reductase-like; 1.
DR PROSITE; PS00075; DHFR_1; 1.
DR PROSITE; PS51330; DHFR_2; 1.
PE 3: Inferred from homology;
KW NADP {ECO:0000256|ARBA:ARBA00022857, ECO:0000256|PIRNR:PIRNR000194};
KW One-carbon metabolism {ECO:0000256|PIRNR:PIRNR000194};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|PIRNR:PIRNR000194}; Plasmid {ECO:0000313|EMBL:CAY33090.1}.
FT DOMAIN 2..160
FT /note="DHFR"
FT /evidence="ECO:0000259|PROSITE:PS51330"
SQ SEQUENCE 163 AA; 19257 MW; 5883637DC360A656 CRC64;
MKVSLIAAMD KNRVIGKEND IPWRIPEDWE YVKNTTKGYP IILGRKNLES IGRALPGRRN
IILTRDKGFS FNGCEIVHSI EDVFELCNSE EEIFIFGGEQ IYNLFLPYVE KMYITKIHYE
FEGDTFFPEV NYEEWNEVSV TQGITNEKNP YTYYFHIYER KAS
//
