ID C9DIG7_TOXGO Unreviewed; 262 AA.
AC C9DIG7;
DT 03-NOV-2009, integrated into UniProtKB/TrEMBL.
DT 03-NOV-2009, sequence version 1.
DT 24-JAN-2024, entry version 48.
DE RecName: Full=Bifunctional dihydrofolate reductase-thymidylate synthase {ECO:0000256|ARBA:ARBA00019798};
DE EC=1.5.1.3 {ECO:0000256|ARBA:ARBA00012856};
DE EC=2.1.1.45 {ECO:0000256|ARBA:ARBA00011947};
DE Flags: Fragment;
GN Name=dhfr {ECO:0000313|EMBL:ACV86017.1};
OS Toxoplasma gondii.
OC Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma.
OX NCBI_TaxID=5811 {ECO:0000313|EMBL:ACV86017.1};
RN [1] {ECO:0000313|EMBL:ACV86017.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=B1 {ECO:0000313|EMBL:ACV86018.1}, ENT
RC {ECO:0000313|EMBL:ACV86017.1}, GRE-1995-MAE
RC {ECO:0000313|EMBL:ACV86021.1}, GRE-1998-TRA
RC {ECO:0000313|EMBL:ACV86019.1}, GUY-2003-MEL
RC {ECO:0000313|EMBL:ACV86022.1}, NED {ECO:0000313|EMBL:ACV86024.1},
RC PSP-2005-MUP {ECO:0000313|EMBL:ACV86023.1}, RMS-1994-LEF
RC {ECO:0000313|EMBL:ACV86031.1}, RMS-1995-ABE
RC {ECO:0000313|EMBL:ACV86028.1}, RMS-2001-MAU
RC {ECO:0000313|EMBL:ACV86029.1}, RMS-2003-DJO
RC {ECO:0000313|EMBL:ACV86030.1}, RMS-2003-TOU
RC {ECO:0000313|EMBL:ACV86027.1}, RMS-2005-HAG
RC {ECO:0000313|EMBL:ACV86020.1}, TOU-1998-TRI
RC {ECO:0000313|EMBL:ACV86026.1}, and TRS-2004-REV
RC {ECO:0000313|EMBL:ACV86025.1};
RA Meneceur P., Bouldouyre M.A., Aubert D., Villena I., Menotti J.,
RA Sauvage V., Garin J.F., Derouin F.;
RT "In vitro susceptibility of various genotypic strains of Toxoplasma gondii
RT to pyrimethamine, sulfadiazine, and atovaquone.";
RL Antimicrob. Agents Chemother. 52:1269-1277(2008).
RN [2] {ECO:0000313|EMBL:ACV86017.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=B1 {ECO:0000313|EMBL:ACV86018.1}, ENT
RC {ECO:0000313|EMBL:ACV86017.1}, GRE-1995-MAE
RC {ECO:0000313|EMBL:ACV86021.1}, GRE-1998-TRA
RC {ECO:0000313|EMBL:ACV86019.1}, GUY-2003-MEL
RC {ECO:0000313|EMBL:ACV86022.1}, NED {ECO:0000313|EMBL:ACV86024.1},
RC PSP-2005-MUP {ECO:0000313|EMBL:ACV86023.1}, RMS-1994-LEF
RC {ECO:0000313|EMBL:ACV86031.1}, RMS-1995-ABE
RC {ECO:0000313|EMBL:ACV86028.1}, RMS-2001-MAU
RC {ECO:0000313|EMBL:ACV86029.1}, RMS-2003-DJO
RC {ECO:0000313|EMBL:ACV86030.1}, RMS-2003-TOU
RC {ECO:0000313|EMBL:ACV86027.1}, RMS-2005-HAG
RC {ECO:0000313|EMBL:ACV86020.1}, TOU-1998-TRI
RC {ECO:0000313|EMBL:ACV86026.1}, and TRS-2004-REV
RC {ECO:0000313|EMBL:ACV86025.1};
RA Sauvage V., Aubert D., Villena I.;
RL Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Bifunctional enzyme. Involved in de novo dTMP biosynthesis.
CC Key enzyme in folate metabolism. Catalyzes an essential reaction for de
CC novo glycine and purine synthesis, DNA precursor synthesis, and for the
CC conversion of dUMP to dTMP. {ECO:0000256|ARBA:ARBA00025154}.
CC -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-
CC tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
CC {ECO:0000256|ARBA:ARBA00004903}.
CC -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC {ECO:0000256|RuleBase:RU004474}.
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DR EMBL; GQ395773; ACV86017.1; -; Genomic_DNA.
DR EMBL; GQ395774; ACV86018.1; -; Genomic_DNA.
DR EMBL; GQ395775; ACV86019.1; -; Genomic_DNA.
DR EMBL; GQ395776; ACV86020.1; -; Genomic_DNA.
DR EMBL; GQ395777; ACV86021.1; -; Genomic_DNA.
DR EMBL; GQ397452; ACV86022.1; -; Genomic_DNA.
DR EMBL; GQ397453; ACV86023.1; -; Genomic_DNA.
DR EMBL; GQ397454; ACV86024.1; -; Genomic_DNA.
DR EMBL; GQ397455; ACV86025.1; -; Genomic_DNA.
DR EMBL; GQ397456; ACV86026.1; -; Genomic_DNA.
DR EMBL; GQ397457; ACV86027.1; -; Genomic_DNA.
DR EMBL; GQ397458; ACV86028.1; -; Genomic_DNA.
DR EMBL; GQ397459; ACV86029.1; -; Genomic_DNA.
DR EMBL; GQ397460; ACV86030.1; -; Genomic_DNA.
DR EMBL; GQ397461; ACV86031.1; -; Genomic_DNA.
DR AlphaFoldDB; C9DIG7; -.
DR SMR; C9DIG7; -.
DR VEuPathDB; ToxoDB:TGARI_249180; -.
DR VEuPathDB; ToxoDB:TGCAST_249180; -.
DR VEuPathDB; ToxoDB:TGCOUG_249180; -.
DR VEuPathDB; ToxoDB:TGDOM2_249180; -.
DR VEuPathDB; ToxoDB:TGFOU_249180; -.
DR VEuPathDB; ToxoDB:TGGT1_249180; -.
DR VEuPathDB; ToxoDB:TGMAS_249180; -.
DR VEuPathDB; ToxoDB:TGME49_249180; -.
DR VEuPathDB; ToxoDB:TGP89_249180; -.
DR VEuPathDB; ToxoDB:TGPRC2_249180; -.
DR VEuPathDB; ToxoDB:TGRH88_062920; -.
DR VEuPathDB; ToxoDB:TGRUB_249180; -.
DR VEuPathDB; ToxoDB:TGVAND_249180; -.
DR VEuPathDB; ToxoDB:TGVEG_249180; -.
DR UniPathway; UPA00077; UER00158.
DR GO; GO:0004146; F:dihydrofolate reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0004799; F:thymidylate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0046654; P:tetrahydrofolate biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00209; DHFR; 1.
DR Gene3D; 3.40.430.10; Dihydrofolate Reductase, subunit A; 1.
DR InterPro; IPR012259; DHFR.
DR InterPro; IPR024072; DHFR-like_dom_sf.
DR InterPro; IPR017925; DHFR_CS.
DR InterPro; IPR001796; DHFR_dom.
DR PANTHER; PTHR48069; DIHYDROFOLATE REDUCTASE; 1.
DR PANTHER; PTHR48069:SF3; DIHYDROFOLATE REDUCTASE; 1.
DR Pfam; PF00186; DHFR_1; 1.
DR PRINTS; PR00070; DHFR.
DR SUPFAM; SSF53597; Dihydrofolate reductase-like; 1.
DR PROSITE; PS00075; DHFR_1; 1.
DR PROSITE; PS51330; DHFR_2; 1.
PE 3: Inferred from homology;
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 4..250
FT /note="DHFR"
FT /evidence="ECO:0000259|PROSITE:PS51330"
FT NON_TER 262
FT /evidence="ECO:0000313|EMBL:ACV86017.1"
SQ SEQUENCE 262 AA; 28832 MW; F68A93BE222D1609 CRC64;
MQKPVCLVVA MTPKRGIGIN NGLPWPHLTT DFKHFSRVTK TTPEEASRLN GWLPRKFAKT
GDSGLPSPSV GKRFNAVVMG RKTWESMPRK FRPLVDRLNI VVSSSLKEED IAAEKPQAEG
QQRVRVCASL PAALSLLEEE YKDSVDQIFV VGGAGLYEAA LSLGVASHLY ITRVAREFPC
DVFFPAFPGD DILSNKSTAA QAAAPAESVF VPFCPELGRE KDNEATYRPI FISKTFSDNG
VPYDFVVLEK RRKTDDAATA EP
//
