ID C9EFI1_9NOCA Unreviewed; 411 AA.
AC C9EFI1;
DT 03-NOV-2009, integrated into UniProtKB/TrEMBL.
DT 03-NOV-2009, sequence version 1.
DT 27-MAR-2024, entry version 49.
DE RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
DE Flags: Fragment;
GN Name=gyrB {ECO:0000313|EMBL:ACV89683.1};
OS Nocardia blacklockiae.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC Nocardia.
OX NCBI_TaxID=480036 {ECO:0000313|EMBL:ACV89683.1};
RN [1] {ECO:0000313|EMBL:ACV89683.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 700035 {ECO:0000313|EMBL:ACV89683.1};
RA Moser B.D., Lasker B.A., Steigerwalt A.G., Hinrikson H.P., Brown J.M.;
RT "Nocardia niwae, sp. nov., isolated from human pulmonary sources.";
RL Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrB family.
CC {ECO:0000256|ARBA:ARBA00010708}.
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DR EMBL; GQ496126; ACV89683.1; -; Genomic_DNA.
DR AlphaFoldDB; C9EFI1; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0034335; F:DNA negative supercoiling activity; IEA:UniProt.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00822; TopoII_Trans_DNA_gyrase; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.40.50.670; -; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR014721; Ribsml_uS5_D2-typ_fold_subgr.
DR InterPro; IPR001241; Topo_IIA.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR000565; Topo_IIA_B.
DR InterPro; IPR013759; Topo_IIA_B_C.
DR InterPro; IPR013506; Topo_IIA_bsu_dom2.
DR InterPro; IPR018522; TopoIIA_CS.
DR InterPro; IPR006171; TOPRIM_domain.
DR PANTHER; PTHR45866:SF1; DNA GYRASE SUBUNIT B, MITOCHONDRIAL; 1.
DR PANTHER; PTHR45866; DNA GYRASE/TOPOISOMERASE SUBUNIT B; 1.
DR Pfam; PF00204; DNA_gyraseB; 1.
DR Pfam; PF01751; Toprim; 1.
DR PRINTS; PR01159; DNAGYRASEB.
DR PRINTS; PR00418; TPI2FAMILY.
DR SMART; SM00433; TOP2c; 1.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1.
DR SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
DR PROSITE; PS00177; TOPOISOMERASE_II; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 141..310
FT /note="DNA topoisomerase type IIA subunit B"
FT /evidence="ECO:0000259|Pfam:PF00204"
FT DOMAIN 339..393
FT /note="Toprim"
FT /evidence="ECO:0000259|Pfam:PF01751"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ACV89683.1"
FT NON_TER 411
FT /evidence="ECO:0000313|EMBL:ACV89683.1"
SQ SEQUENCE 411 AA; 45226 MW; 2C7FB41E8FFD7058 CRC64;
SDAYAVSGGL HGVGISVVNA LSTRMVTEID RDGFHWSQEY KDSKPGKLVK GEPTKRTGTT
QRFWADPAIF ETTTYNFETI ARRLQEMAFL NKGLTITLTD ERVSDAEITD EVVSDTAEAP
KAEAEPVEHK VKTRTYHYPG GLADFVKHIN RSKQPIHNTV VSFTGKGTGH ELEVAMQWNS
GYSESVHTFA NTINTHEGGT HEEGFRAALT SVVNKYAKDK KLIKDKDGNL TGDDIREGLA
AIVSVKVSDP QFEGQTKTKL GNTEVKSFVQ RTCNEHLAHW LEANPADAKT IVQKAVSSAQ
ARVAARKARE LVRRKSATDL GGLPGKLADC RSKDPRLSEI YIVEGDSAGG SAKSGRDSMY
QAILPIRGKI INVEKSRIDK VLKNNEVQSI ITAFGTGIHD EFDISKLRYH K
//
