ID CHS9_DAUCA Reviewed; 389 AA.
AC Q9SB26;
DT 21-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 27-MAR-2024, entry version 79.
DE RecName: Full=Chalcone synthase 9;
DE EC=2.3.1.74;
DE AltName: Full=Naringenin-chalcone synthase 9;
GN Name=CHS9;
OS Daucus carota (Wild carrot).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC Daucus; Daucus sect. Daucus.
OX NCBI_TaxID=4039;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Kurodagosun;
RA Ozeki Y., Davies E., Takeda J.;
RT "Structure and expression of chalcone synthase gene in carrot suspension
RT cultured cells regulated by 2,4-D.";
RL Plant Cell Physiol. 34:1029-1037(1993).
CC -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC which can under specific conditions spontaneously isomerize into
CC naringenin.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000305}.
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DR EMBL; D16256; BAA03785.1; -; mRNA.
DR AlphaFoldDB; Q9SB26; -.
DR SMR; Q9SB26; -.
DR UniPathway; UPA00154; -.
DR GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877:SF14; CHALCONE SYNTHASE; 1.
DR PANTHER; PTHR11877; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Flavonoid biosynthesis; Transferase.
FT CHAIN 1..389
FT /note="Chalcone synthase 9"
FT /id="PRO_0000215972"
FT ACT_SITE 164
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ SEQUENCE 389 AA; 42711 MW; 50EC12B35FDD55F0 CRC64;
MVTVNEFRKA QRAEGPATVL AIGTATPPNC VDQSAYADYY FRITNSEDKP ELKEKFRRMC
EKSMINTRYM HLTEDLLKQN PSFCEYMASS LDARQDIVVN EVPKLGKEAA LRAIKEWGRP
KSKITHLIFC TTSGVDMPGA DFRLTKLLGL RPSVKRFMMY QQGCFAGGTV LRLAKDLAEN
NKNARVLVVC SEITVITFRG PNDTHLDSLV GQALFGDGAG AVIVGSDPVI GIEKPLFEIV
SAAQTILPDS DGAIDGHLRE VGLTFHLLKD VPGLISKNIR KSLVEAFKPL GISDWNSIFW
IAHPGGPAIL DQVETELSLK PDKLKSTRQV LRDYGNMSSA CVLFILDEMR NASAKDGHRT
TGEGLDWGVL FGFGPGLTVE TVVLHSVPT
//
