ID CKB2_CAEEL Reviewed; 369 AA.
AC P46559; Q8IS23;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2004, sequence version 2.
DT 27-MAR-2024, entry version 140.
DE RecName: Full=Choline kinase B2 {ECO:0000303|PubMed:12758145};
DE EC=2.7.1.32 {ECO:0000269|PubMed:12758145};
GN Name=ckb-2; ORFNames=B0285.9;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, COFACTOR,
RP BIOPHYSICOCHEMICAL PROPERTIES, AND PATHWAY.
RX PubMed=12758145; DOI=10.1016/s1570-9639(03)00106-7;
RA Gee P., Kent C.;
RT "Multiple isoforms of choline kinase from Caenorhabditis elegans: cloning,
RT expression, purification, and characterization.";
RL Biochim. Biophys. Acta 1648:33-42(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Catalyzes the first step in phosphatidylcholine biosynthesis.
CC Phosphorylates choline. {ECO:0000269|PubMed:12758145}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + choline = ADP + H(+) + phosphocholine;
CC Xref=Rhea:RHEA:12837, ChEBI:CHEBI:15354, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:295975, ChEBI:CHEBI:456216;
CC EC=2.7.1.32; Evidence={ECO:0000269|PubMed:12758145};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000269|PubMed:12758145};
CC Note=Less efficient with Mn(2+). {ECO:0000269|PubMed:12758145};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=13 mM for choline (at 37 degrees Celsius and pH 10)
CC {ECO:0000269|PubMed:12758145};
CC KM=0.72 mM for ATP (at 37 degrees Celsius and pH 10)
CC {ECO:0000269|PubMed:12758145};
CC Vmax=5.4 umol/min/mg enzyme (at 37 degrees Celsius and pH 10)
CC {ECO:0000269|PubMed:12758145};
CC pH dependence:
CC Optimum pH is 10. {ECO:0000269|PubMed:12758145};
CC -!- PATHWAY: Phospholipid metabolism; phosphatidylcholine biosynthesis;
CC phosphocholine from choline: step 1/1. {ECO:0000269|PubMed:12758145}.
CC -!- SIMILARITY: Belongs to the choline/ethanolamine kinase family.
CC {ECO:0000305}.
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DR EMBL; AY158691; AAN41643.1; -; mRNA.
DR EMBL; Z34533; CAA84301.2; -; Genomic_DNA.
DR PIR; T18696; T18696.
DR RefSeq; NP_497880.2; NM_065479.6.
DR AlphaFoldDB; P46559; -.
DR SMR; P46559; -.
DR BioGRID; 40801; 4.
DR STRING; 6239.B0285.9.2; -.
DR iPTMnet; P46559; -.
DR EPD; P46559; -.
DR PaxDb; 6239-B0285-9; -.
DR PeptideAtlas; P46559; -.
DR EnsemblMetazoa; B0285.9.1; B0285.9.1; WBGene00000512.
DR GeneID; 175565; -.
DR KEGG; cel:CELE_B0285.9; -.
DR UCSC; B0285.9; c. elegans.
DR AGR; WB:WBGene00000512; -.
DR WormBase; B0285.9; CE33591; WBGene00000512; ckb-2.
DR eggNOG; KOG2686; Eukaryota.
DR GeneTree; ENSGT00950000182939; -.
DR HOGENOM; CLU_012712_2_0_1; -.
DR InParanoid; P46559; -.
DR OMA; WSTELYE; -.
DR OrthoDB; 144299at2759; -.
DR PhylomeDB; P46559; -.
DR Reactome; R-CEL-1483191; Synthesis of PC.
DR Reactome; R-CEL-1483213; Synthesis of PE.
DR UniPathway; UPA00753; UER00737.
DR PRO; PR:P46559; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00000512; Expressed in germ line (C elegans) and 4 other cell types or tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004103; F:choline kinase activity; IDA:WormBase.
DR GO; GO:0004305; F:ethanolamine kinase activity; IBA:GO_Central.
DR GO; GO:0006657; P:CDP-choline pathway; IDA:WormBase.
DR GO; GO:0030968; P:endoplasmic reticulum unfolded protein response; HEP:WormBase.
DR GO; GO:0006646; P:phosphatidylethanolamine biosynthetic process; IBA:GO_Central.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR Gene3D; 3.90.1200.10; -; 1.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR PANTHER; PTHR22603:SF25; CHOLINE KINASE B1-RELATED; 1.
DR PANTHER; PTHR22603; CHOLINE/ETHANOALAMINE KINASE; 1.
DR Pfam; PF01633; Choline_kinase; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
PE 1: Evidence at protein level;
KW ATP-binding; Kinase; Lipid biosynthesis; Lipid metabolism; Magnesium;
KW Nucleotide-binding; Phospholipid biosynthesis; Phospholipid metabolism;
KW Reference proteome; Transferase.
FT CHAIN 1..369
FT /note="Choline kinase B2"
FT /id="PRO_0000206233"
SQ SEQUENCE 369 AA; 42264 MW; 24384022ED1458B3 CRC64;
MTAIEKFFTE KSPDSEQVLL KVIELGIDFL GGEWKNVDKS QVNVSRVHGG QSNHMFHVTS
STSATPYLLR IHRQPPSQVF TDTVNLAIFS ERGLGPKLYG FFEGGRMEEF LPSKTFDVND
VLVPENSRKI GAIFPLYHSI NVPVSKSRRC VHLMREWLNG YESLGGGDYE ILPTTVNYSD
HPKSVSIKDL NHEIDNFEKW STEIFEHTLV FSHNDLASTN ILELNSTKEL VLIDWEFGTY
NWRGFDLAMH LSETAIDYRV PFPPGIKMNG DLIDNPPNIQ IFCEAYVEAD KKLKNRSPSD
PTAEVKALIQ ECQFFWPLTN LFWALSAMKH SLLKFENGVD LDVQARDRLA VYFHLKPRSQ
KIYEELSKK
//
