GenomeNet

Database: UniProt
Entry: CN15A_MEDTR
LinkDB: CN15A_MEDTR
Original site: CN15A_MEDTR 
ID   CN15A_MEDTR             Reviewed;         710 AA.
AC   G7IBJ4;
DT   07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT   25-JAN-2012, sequence version 1.
DT   07-JUN-2017, entry version 35.
DE   RecName: Full=Protein CNGC15a {ECO:0000303|PubMed:27230377};
DE   AltName: Full=Cyclic nucleotide-gated ion channel protein 15 a {ECO:0000303|PubMed:27230377};
GN   Name=CNGC15A {ECO:0000303|PubMed:27230377};
GN   OrderedLocusNames=MTR_1g064240 {ECO:0000312|EnsemblPlants:AES60733};
OS   Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae;
OC   Trifolieae; Medicago.
OX   NCBI_TaxID=3880 {ECO:0000312|Proteomes:UP000002051};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Jemalong A17;
RX   PubMed=22089132; DOI=10.1038/nature10625;
RA   Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA   Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA   Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M.,
RA   Cheung F., De Mita S., Krishnakumar V., Gundlach H., Zhou S.,
RA   Mudge J., Bharti A.K., Murray J.D., Naoumkina M.A., Rosen B.,
RA   Silverstein K.A.T., Tang H., Rombauts S., Zhao P.X., Zhou P.,
RA   Barbe V., Bardou P., Bechner M., Bellec A., Berger A., Berges H.,
RA   Bidwell S., Bisseling T., Choisne N., Couloux A., Denny R.,
RA   Deshpande S., Dai X., Doyle J.J., Dudez A.-M., Farmer A.D.,
RA   Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA   Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A.,
RA   Humphray S.J., Jeong D.-H., Jing Y., Jocker A., Kenton S.M.,
RA   Kim D.-J., Klee K., Lai H., Lang C., Lin S., Macmil S.L.,
RA   Magdelenat G., Matthews L., McCorrison J., Monaghan E.L., Mun J.-H.,
RA   Najar F.Z., Nicholson C., Noirot C., O'Bleness M., Paule C.R.,
RA   Poulain J., Prion F., Qin B., Qu C., Retzel E.F., Riddle C.,
RA   Sallet E., Samain S., Samson N., Sanders I., Saurat O., Scarpelli C.,
RA   Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R., Sims S.,
RA   Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B., Wang K.,
RA   Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA   White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA   Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA   Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT   "The Medicago genome provides insight into the evolution of rhizobial
RT   symbioses.";
RL   Nature 480:520-524(2011).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Jemalong A17;
RX   PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA   Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA   Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA   Schwartz D.C., Town C.D.;
RT   "An improved genome release (version Mt4.0) for the model legume
RT   Medicago truncatula.";
RL   BMC Genomics 15:312-312(2014).
RN   [3]
RP   FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH
RP   DMI1, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=27230377; DOI=10.1126/science.aae0109;
RA   Charpentier M., Sun J., Martins T.V., Radhakrishnan G.V., Findlay K.,
RA   Soumpourou E., Thouin J., Very A.A., Sanders D., Morris R.J.,
RA   Oldroyd G.E.;
RT   "Nuclear-localized cyclic nucleotide-gated channels mediate symbiotic
RT   calcium oscillations.";
RL   Science 352:1102-1105(2016).
CC   -!- FUNCTION: Cyclic nucleotide-gated channel involved in the
CC       establishment of both rhizobial and mycorrhizal associations
CC       (PubMed:27230377). Required for full activation of nuclear-
CC       localized Ca(2+) oscillations by Nod and Myc factors
CC       (PubMed:27230377). Simultaneous activation of the K(+)-permeable
CC       channel DMI1 and the Ca(2+) channel CNGC15 can give rise to
CC       sustained Ca(2+) oscillations (PubMed:27230377). May function
CC       during fertilization in both female and male gametophytic Ca(2+)
CC       signaling (PubMed:27230377). {ECO:0000269|PubMed:27230377}.
CC   -!- SUBUNIT: Interacts (via N-terminus) with DMI1 (via c-terminus).
CC       The Nod factor has no effect on this interaction, implying that
CC       the complex is maintained after activation.
CC       {ECO:0000269|PubMed:27230377}.
CC   -!- SUBCELLULAR LOCATION: Nucleus membrane
CC       {ECO:0000269|PubMed:27230377}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, stems, leaves, flowers and
CC       pods. {ECO:0000269|PubMed:27230377}.
CC   -!- SIMILARITY: Belongs to the cyclic nucleotide-gated cation channel
CC       (TC 1.A.1.5) family. {ECO:0000305}.
DR   EMBL; CM001217; AES60733.1; -; Genomic_DNA.
DR   RefSeq; XP_003590482.1; XM_003590434.2.
DR   SMR; G7IBJ4; -.
DR   EnsemblPlants; AES60733; AES60733; MTR_1g064240.
DR   GeneID; 11433273; -.
DR   Gramene; AES60733; AES60733; MTR_1g064240.
DR   KEGG; mtr:MTR_1g064240; -.
DR   KO; K05391; -.
DR   OMA; LCTPGTC; -.
DR   OrthoDB; EOG093603GN; -.
DR   Proteomes; UP000002051; Chromosome 1.
DR   Proteomes; UP000002051; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031965; C:nuclear membrane; IDA:UniProtKB.
DR   GO; GO:0005262; F:calcium channel activity; IDA:UniProtKB.
DR   GO; GO:0044325; F:ion channel binding; IPI:UniProtKB.
DR   GO; GO:0005249; F:voltage-gated potassium channel activity; IEA:InterPro.
DR   GO; GO:0036377; P:arbuscular mycorrhizal association; IMP:UniProtKB.
DR   GO; GO:0009877; P:nodulation; IMP:UniProtKB.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR018490; cNMP-bd-like.
DR   InterPro; IPR000595; cNMP-bd_dom.
DR   InterPro; IPR005821; Ion_trans_dom.
DR   InterPro; IPR003938; K_chnl_volt-dep_EAG/ELK/ERG.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   Pfam; PF00027; cNMP_binding; 1.
DR   Pfam; PF00520; Ion_trans; 1.
DR   PRINTS; PR01463; EAGCHANLFMLY.
DR   SMART; SM00100; cNMP; 1.
DR   SUPFAM; SSF51206; SSF51206; 1.
DR   PROSITE; PS50042; CNMP_BINDING_3; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Ion channel; Ion transport;
KW   Ligand-gated ion channel; Membrane; Nucleus; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN         1    710       Protein CNGC15a.
FT                                /FTId=PRO_0000437092.
FT   TRANSMEM     85    105       Helical. {ECO:0000255}.
FT   TRANSMEM    115    135       Helical. {ECO:0000255}.
FT   TRANSMEM    174    194       Helical. {ECO:0000255}.
FT   TRANSMEM    207    226       Helical. {ECO:0000255}.
FT   TRANSMEM    248    268       Helical. {ECO:0000255}.
FT   TRANSMEM    368    388       Helical. {ECO:0000255}.
FT   NP_BIND     474    559       cNMP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00060}.
SQ   SEQUENCE   710 AA;  81946 MW;  1CFA701DD65423D9 CRC64;
     MASVISRAVR FHDDLEKEKL QEGEESHMEM RAYEMSSEYK HGKDAINKPS SNGRGLSRVF
     SEDYDAGEIL VFDPRGPRIN LWNKIFLAAC LISLFVDPLF FYLPVAKKEK CIDMSIGLEV
     SLTIIRTFVD AFYIIHIYIR FQTAYIAPSS RVSGRGELII DSSKIASNYM KKELWSDLVA
     ALPLPQVLIW AVIPNIKGSE MIASRHVVRL VSIFQYLLRL YLIYPLSSKI TKASGVMMEK
     AWAGAAYYLT LYMLASHVLG STWYLLSIER QDECWKKACT LQYPHCQYHY LDCQSLSDPN
     RNAWLKSSNL SGLCDQNSHF FQFGIFDDAV TLEITSSNFL TKYYYCLWWG LRNLSSSGEN
     LLTSTHVAEI NFAVIVAILG LVLFALLIGN MQTYLQSTTI RLEEWRIRRT DTERWMHHRQ
     LPHYLKENVR RHDQFRWVAT RGVDEEAILR DLPVDLRRDI KRHLCLNLVR QVPLFDQMDD
     RMLDAICERL KPTLCTPGTC IVREGDPVDE MLFIVRGRLD SCTTNGGRTG FFNTCRIGSG
     DFCGEELLPW ALDPRPTAVL PSSTRTVRAI TEVEAFALIA EDLKFVAAQF RRLHSKQLRQ
     TFRFYSHQWR TWAACFIQAA WFRYKRMKET NEVKEKENLM MMSNVKYYGN DDSQYFSAPL
     QVPKGSSYSM YSGKLVGSLR RGRSMRYGSE LDMLGTLRKP IEPDFNDDGD
//
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