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Database: UniProt
Entry: CN15C_MEDTR
LinkDB: CN15C_MEDTR
Original site: CN15C_MEDTR 
ID   CN15C_MEDTR             Reviewed;         703 AA.
AC   A0A072VMJ3;
DT   07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2014, sequence version 1.
DT   10-MAY-2017, entry version 19.
DE   RecName: Full=Protein CNGC15c {ECO:0000303|PubMed:27230377};
DE   AltName: Full=Cyclic nucleotide-gated ion channel protein 15 c {ECO:0000303|PubMed:27230377};
GN   Name=CNGC15C {ECO:0000303|PubMed:27230377};
GN   OrderedLocusNames=MTR_2g094860 {ECO:0000312|EnsemblPlants:KEH39345};
OS   Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae;
OC   Trifolieae; Medicago.
OX   NCBI_TaxID=3880 {ECO:0000312|Proteomes:UP000002051};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Jemalong A17;
RX   PubMed=22089132; DOI=10.1038/nature10625;
RA   Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA   Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA   Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M.,
RA   Cheung F., De Mita S., Krishnakumar V., Gundlach H., Zhou S.,
RA   Mudge J., Bharti A.K., Murray J.D., Naoumkina M.A., Rosen B.,
RA   Silverstein K.A.T., Tang H., Rombauts S., Zhao P.X., Zhou P.,
RA   Barbe V., Bardou P., Bechner M., Bellec A., Berger A., Berges H.,
RA   Bidwell S., Bisseling T., Choisne N., Couloux A., Denny R.,
RA   Deshpande S., Dai X., Doyle J.J., Dudez A.-M., Farmer A.D.,
RA   Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA   Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A.,
RA   Humphray S.J., Jeong D.-H., Jing Y., Jocker A., Kenton S.M.,
RA   Kim D.-J., Klee K., Lai H., Lang C., Lin S., Macmil S.L.,
RA   Magdelenat G., Matthews L., McCorrison J., Monaghan E.L., Mun J.-H.,
RA   Najar F.Z., Nicholson C., Noirot C., O'Bleness M., Paule C.R.,
RA   Poulain J., Prion F., Qin B., Qu C., Retzel E.F., Riddle C.,
RA   Sallet E., Samain S., Samson N., Sanders I., Saurat O., Scarpelli C.,
RA   Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R., Sims S.,
RA   Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B., Wang K.,
RA   Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA   White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA   Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA   Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT   "The Medicago genome provides insight into the evolution of rhizobial
RT   symbioses.";
RL   Nature 480:520-524(2011).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Jemalong A17;
RX   PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA   Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA   Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA   Schwartz D.C., Town C.D.;
RT   "An improved genome release (version Mt4.0) for the model legume
RT   Medicago truncatula.";
RL   BMC Genomics 15:312-312(2014).
RN   [3]
RP   FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH
RP   DMI1, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=27230377; DOI=10.1126/science.aae0109;
RA   Charpentier M., Sun J., Martins T.V., Radhakrishnan G.V., Findlay K.,
RA   Soumpourou E., Thouin J., Very A.A., Sanders D., Morris R.J.,
RA   Oldroyd G.E.;
RT   "Nuclear-localized cyclic nucleotide-gated channels mediate symbiotic
RT   calcium oscillations.";
RL   Science 352:1102-1105(2016).
CC   -!- FUNCTION: Cyclic nucleotide-gated channel involved in the
CC       establishment of both rhizobial and mycorrhizal associations
CC       (PubMed:27230377). Required for full activation of nuclear-
CC       localized Ca(2+) oscillations by Nod and Myc factors
CC       (PubMed:27230377). Simultaneous activation of the K(+)-permeable
CC       channel DMI1 and the Ca(2+) channel CNGC15 can give rise to
CC       sustained Ca(2+) oscillations (PubMed:27230377). May function
CC       during fertilization in both female and male gametophytic Ca(2+)
CC       signaling (PubMed:27230377). {ECO:0000269|PubMed:27230377}.
CC   -!- SUBUNIT: Interacts (via N-terminus) with DMI1 (via c-terminus).
CC       The Nod factor has no effect on this interaction, implying that
CC       the complex is maintained after activation.
CC       {ECO:0000269|PubMed:27230377}.
CC   -!- SUBCELLULAR LOCATION: Nucleus membrane
CC       {ECO:0000269|PubMed:27230377}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, stems, leaves, flowers and
CC       pods. {ECO:0000269|PubMed:27230377}.
CC   -!- SIMILARITY: Belongs to the cyclic nucleotide-gated cation channel
CC       (TC 1.A.1.5) family. {ECO:0000305}.
DR   EMBL; CM001218; KEH39345.1; -; Genomic_DNA.
DR   RefSeq; XP_013465310.1; XM_013609856.1.
DR   EnsemblPlants; KEH39345; KEH39345; MTR_2g094860.
DR   GeneID; 25487817; -.
DR   Gramene; KEH39345; KEH39345; MTR_2g094860.
DR   KEGG; mtr:MTR_2g094860; -.
DR   KO; K05391; -.
DR   Proteomes; UP000002051; Chromosome 2.
DR   Proteomes; UP000002051; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044325; F:ion channel binding; IPI:UniProtKB.
DR   GO; GO:0005249; F:voltage-gated potassium channel activity; IEA:InterPro.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR018490; cNMP-bd-like.
DR   InterPro; IPR000595; cNMP-bd_dom.
DR   InterPro; IPR005821; Ion_trans_dom.
DR   InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR   InterPro; IPR003938; K_chnl_volt-dep_EAG/ELK/ERG.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   Pfam; PF00520; Ion_trans; 1.
DR   PRINTS; PR01463; EAGCHANLFMLY.
DR   SMART; SM00100; cNMP; 1.
DR   SUPFAM; SSF51206; SSF51206; 1.
DR   PROSITE; PS50042; CNMP_BINDING_3; 1.
DR   PROSITE; PS50096; IQ; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Ion channel; Ion transport;
KW   Ligand-gated ion channel; Membrane; Nucleus; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN         1    703       Protein CNGC15c.
FT                                /FTId=PRO_0000437094.
FT   TRANSMEM     91    111       Helical. {ECO:0000255}.
FT   TRANSMEM    179    199       Helical. {ECO:0000255}.
FT   TRANSMEM    216    236       Helical. {ECO:0000255}.
FT   TRANSMEM    255    275       Helical. {ECO:0000255}.
FT   TRANSMEM    372    392       Helical. {ECO:0000255}.
FT   DOMAIN      616    644       IQ. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00116}.
FT   NP_BIND     480    565       cNMP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00060}.
SQ   SEQUENCE   703 AA;  80770 MW;  3914EF145F87AEC4 CRC64;
     MGFDNPRSER FEDDPEISKI PTTSGVKVKY HIDGTQIPEQ SSKKSRKNET RNKFLKTRVL
     SRVFSEDYER VKKRVLVLDP RGQLIHRWNK IFLVACLVSL FVDPLFFYLP VVREEVCIDI
     GKTLEVILTV VRSFGDLFYI VQICMKFRTA YVAPSSKVFG RGELVLTYSK IALRYFSKGF
     WLDFIAALPL PQVLIWIIIP TLRGSTMANT KNVLRFFIIF QYIPRLYLIF PLSSQIVKAT
     GVVTETAWAG AAYNLMLYML ASHILGACWY LLSIERQEAC WKSVCNMEKS NCQYGFFNCH
     SIKDAPRVAW FIASNVTNLC SPNAGFYPFG IYADAMTSKV TSSPFFNKYF YCLWWGLRNL
     SSLGQGLLTS TFIGEIMVAI VVATLGLVLF ALLIGNMQTY LQSITVRLEE WRVKRTDTEQ
     WMHHRQLPPE LRESIRKYNQ YKWVATRGVE EEDLLKGLPL DLRREIKRHL CLELVRGVPL
     FDQMDERMLD AICERLKPAL CTEGTYLVRE GDPVNEMLFI IRGHLDSYTT NGGRDGFFNS
     CRIGPGDFCG EELLTWALDP RPSVILPSST RTVKAFSEVE AFALIAEDLK FVASQFRRLH
     SKQLRHKFRF YSHQWRTWAA CFIQAAWRRH KKRKEAAELR AKENLVAASE AENEIAKKYG
     KGFVVYGTRV ARSTRKGVNM HSGTNSGVVS SLQKPTEPDF SDE
//
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