ID CRX_MOUSE Reviewed; 299 AA.
AC O54751;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 27-MAR-2024, entry version 178.
DE RecName: Full=Cone-rod homeobox protein;
GN Name=Crx;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Retina;
RX PubMed=9390562; DOI=10.1016/s0092-8674(00)80439-0;
RA Furukawa T., Morrow E.M., Cepko C.L.;
RT "Crx, a novel otx-like homeobox gene, shows photoreceptor-specific
RT expression and regulates photoreceptor differentiation.";
RL Cell 91:531-541(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION AS TRANSCRIPTION ACTIVATOR, SUBCELLULAR LOCATION, DNA-BINDING,
RP INTERACTION WITH NRL AND RORB, DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J; TISSUE=Embryonic eye;
RX PubMed=16574740; DOI=10.1210/me.2005-0505;
RA Srinivas M., Ng L., Liu H., Jia L., Forrest D.;
RT "Activation of the blue opsin gene in cone photoreceptor development by
RT retinoid-related orphan receptor beta.";
RL Mol. Endocrinol. 20:1728-1741(2006).
CC -!- FUNCTION: Transcription factor that binds and transactivates the
CC sequence 5'-TAATC[CA]-3' which is found upstream of several
CC photoreceptor-specific genes, including the opsin genes. Acts
CC synergistically with other transcription factors, such as NRL, RORB and
CC RAX, to regulate photoreceptor cell-specific gene transcription.
CC Essential for the maintenance of mammalian photoreceptors.
CC {ECO:0000269|PubMed:16574740}.
CC -!- SUBUNIT: Interacts (via the homeobox) with NRL (via the leucine-zipper
CC domain). Interacts with PDC, RAX2, RORB and SCA7.
CC {ECO:0000269|PubMed:16574740}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000269|PubMed:16574740}.
CC -!- TISSUE SPECIFICITY: Retina. {ECO:0000269|PubMed:16574740}.
CC -!- DEVELOPMENTAL STAGE: At 17.5 dpc, expressed in a narrow zone in the
CC peripheral outer nuclear layer of the retina. At P12, expressed over
CC the entire photoreceptor layer. {ECO:0000269|PubMed:16574740}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. {ECO:0000305}.
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DR EMBL; U77615; AAB88585.1; -; mRNA.
DR EMBL; BC016502; AAH16502.1; -; mRNA.
DR CCDS; CCDS20840.1; -.
DR RefSeq; NP_001106801.1; NM_001113330.1.
DR RefSeq; NP_031796.1; NM_007770.4.
DR AlphaFoldDB; O54751; -.
DR SMR; O54751; -.
DR BioGRID; 198905; 3.
DR STRING; 10090.ENSMUSP00000134400; -.
DR PhosphoSitePlus; O54751; -.
DR PaxDb; 10090-ENSMUSP00000134400; -.
DR ProteomicsDB; 285336; -.
DR Antibodypedia; 31635; 302 antibodies from 33 providers.
DR DNASU; 12951; -.
DR Ensembl; ENSMUST00000044434.13; ENSMUSP00000043436.7; ENSMUSG00000041578.16.
DR GeneID; 12951; -.
DR KEGG; mmu:12951; -.
DR UCSC; uc009fgm.2; mouse.
DR AGR; MGI:1194883; -.
DR CTD; 1406; -.
DR MGI; MGI:1194883; Crx.
DR VEuPathDB; HostDB:ENSMUSG00000041578; -.
DR eggNOG; KOG2251; Eukaryota.
DR GeneTree; ENSGT00940000161634; -.
DR HOGENOM; CLU_064370_0_0_1; -.
DR InParanoid; O54751; -.
DR OMA; TWKFAYN; -.
DR OrthoDB; 2913621at2759; -.
DR PhylomeDB; O54751; -.
DR TreeFam; TF351179; -.
DR BioGRID-ORCS; 12951; 3 hits in 83 CRISPR screens.
DR ChiTaRS; Crx; mouse.
DR PRO; PR:O54751; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; O54751; Protein.
DR Bgee; ENSMUSG00000041578; Expressed in retinal neural layer and 25 other cell types or tissues.
DR ExpressionAtlas; O54751; baseline and differential.
DR Genevisible; O54751; MM.
DR GO; GO:0000785; C:chromatin; ISS:ARUK-UCL.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; ISS:ARUK-UCL.
DR GO; GO:0005667; C:transcription regulator complex; IDA:MGI.
DR GO; GO:0003682; F:chromatin binding; IDA:MGI.
DR GO; GO:0003677; F:DNA binding; ISO:MGI.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; ISS:ARUK-UCL.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0043522; F:leucine zipper domain binding; ISO:MGI.
DR GO; GO:0016922; F:nuclear receptor binding; IPI:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:MGI.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; ISS:ARUK-UCL.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR GO; GO:0060041; P:retina development in camera-type eye; IMP:MGI.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR013851; Otx_TF_C.
DR PANTHER; PTHR45793:SF22; CONE-ROD HOMEOBOX PROTEIN; 1.
DR PANTHER; PTHR45793; HOMEOBOX PROTEIN; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03529; TF_Otx; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; Differentiation; DNA-binding; Homeobox;
KW Neurogenesis; Nucleus; Reference proteome; Sensory transduction;
KW Transcription; Transcription regulation; Vision.
FT CHAIN 1..299
FT /note="Cone-rod homeobox protein"
FT /id="PRO_0000048863"
FT DNA_BIND 39..98
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 94..155
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 236..258
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 126..155
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 241..258
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 299 AA; 32374 MW; 4FE2C27D8070B524 CRC64;
MMAYMNPGPH YSVNALALSG PNVDLMHQAV PYSSAPRKQR RERTTFTRSQ LEELEALFAK
TQYPDVYARE EVALKINLPE SRVQVWFKNR RAKCRQQRQQ QKQQQQPPGA QTKARPAKRK
AGTSPRPSTD VCTDPLGISD SYSPSLPGPS GSPTTAVATV SIWSPASEAP LPEAQRAGLV
ASGPSLTSAP YAMTYAPASA FCSSPSAYAS PSSYFSGLDP YLSPMVPQLG GPALSPLSGP
SVGPSLAQSP TSLSGQSYST YSPVDSLEFK DPTGTWKFTY NPMDPLDYKD QSAWKFQIL
//
