ID CWC21_KLULA Reviewed; 113 AA.
AC Q6CVR3;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 2.
DT 24-JAN-2024, entry version 75.
DE RecName: Full=Pre-mRNA-splicing factor CWC21;
GN Name=CWC21; OrderedLocusNames=KLLA0B10054g;
OS Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX NCBI_TaxID=284590;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
CC -!- FUNCTION: Involved in pre-mRNA splicing. May function at or prior to
CC the first catalytic step of splicing at the catalytic center of the
CC spliceosome. May do so by stabilizing the catalytic center or the
CC position of the RNA substrate (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Associates with the NTC complex (or PRP19-associated complex).
CC The NTC complex associates with the spliceosome after the release of
CC the U1 and U4 snRNAs and forms the CWC spliceosome subcomplex
CC reminiscent of a late-stage spliceosome. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CWC21 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAH02369.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CR382122; CAH02369.1; ALT_INIT; Genomic_DNA.
DR RefSeq; XP_451976.1; XM_451976.1.
DR AlphaFoldDB; Q6CVR3; -.
DR SMR; Q6CVR3; -.
DR STRING; 284590.Q6CVR3; -.
DR PaxDb; 284590-Q6CVR3; -.
DR GeneID; 2897000; -.
DR KEGG; kla:KLLA0_B10054g; -.
DR eggNOG; KOG1869; Eukaryota.
DR HOGENOM; CLU_1731738_0_0_1; -.
DR InParanoid; Q6CVR3; -.
DR Proteomes; UP000000598; Chromosome B.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR CDD; cd21372; cwf21_CWC21-like; 1.
DR Gene3D; 6.10.140.420; -; 1.
DR InterPro; IPR013170; mRNA_splic_Cwf21_dom.
DR PANTHER; PTHR36562:SF5; RHODANESE DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR36562; SERINE/ARGININE REPETITIVE MATRIX 2; 1.
DR Pfam; PF08312; cwf21; 1.
DR SMART; SM01115; cwf21; 1.
PE 3: Inferred from homology;
KW Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Spliceosome.
FT CHAIN 1..113
FT /note="Pre-mRNA-splicing factor CWC21"
FT /id="PRO_0000123500"
FT DOMAIN 60..105
FT /note="CWF21"
FT /evidence="ECO:0000255"
FT REGION 1..61
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 40..56
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 113 AA; 12862 MW; 7EC19312311C73F9 CRC64;
MSYNGIGLSS AKGSSTSGYV QQSLAFTNRK KDPRLTTHDK EQQQQQQQQQ IQPAKDESVI
SHKAKRQIEL LVSEYRDKLE DGPDDLSDDT IDSKCEDYRR SILEGNEQSR SQN
//
