ID D0BLC3_9LACT Unreviewed; 596 AA.
AC D0BLC3;
DT 24-NOV-2009, integrated into UniProtKB/TrEMBL.
DT 24-NOV-2009, sequence version 1.
DT 24-JAN-2024, entry version 47.
DE RecName: Full=Penicillin-binding protein 2B {ECO:0008006|Google:ProtNLM};
GN ORFNames=HMPREF0446_00758 {ECO:0000313|EMBL:EEW93876.1};
OS Granulicatella elegans ATCC 700633.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
OC Granulicatella.
OX NCBI_TaxID=626369 {ECO:0000313|EMBL:EEW93876.1, ECO:0000313|Proteomes:UP000002939};
RN [1] {ECO:0000313|EMBL:EEW93876.1, ECO:0000313|Proteomes:UP000002939}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700633 {ECO:0000313|EMBL:EEW93876.1,
RC ECO:0000313|Proteomes:UP000002939};
RG The Broad Institute Genome Sequencing Platform;
RA Ward D., Feldgarden M., Earl A., Young S.K., Zeng Q., Koehrsen M.,
RA Alvarado L., Berlin A., Bochicchio J., Borenstein D., Chapman S.B.,
RA Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A.,
RA Gujja S., Heilman E., Heiman D., Hepburn T., Howarth C., Jen D., Larson L.,
RA Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shea T.,
RA Shenoy N., Sisk P., Stolte C., Sykes S., Thomson T., Walk T., White J.,
RA Yandava C., Sibley C.D., Field T.R., Grinwis M., Eshaghurshan C.S.,
RA Surette M.G., Haas B., Nusbaum C., Birren B.;
RL Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EEW93876.1, ECO:0000313|Proteomes:UP000002939}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700633 {ECO:0000313|EMBL:EEW93876.1,
RC ECO:0000313|Proteomes:UP000002939};
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A., Ward D., Feldgarden M., Gevers D., Sibley C.D., Field T.R.,
RA Grinwis M., Eshaghurshan C.S., Surette M.G., Young S.K., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A., Brown A., Chapman S.B., Chen Z., Dunbar C.,
RA Freedman E., Gearin G., Goldberg J., Griggs A., Gujja S., Heiman D.,
RA Howarth C., Larson L., Lui A., MacDonald P.J.P., Montmayeur A., Murphy C.,
RA Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T., Shenoy N.,
RA Sisk P., Stolte C., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Granulicatella elegans ATCC 700633.";
RL Submitted (OCT-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004162};
CC Single-pass membrane protein {ECO:0000256|ARBA:ARBA00004162}. Membrane
CC {ECO:0000256|ARBA:ARBA00004167}; Single-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004167}.
CC -!- SIMILARITY: Belongs to the transpeptidase family.
CC {ECO:0000256|ARBA:ARBA00007171}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEW93876.1}.
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DR EMBL; ACRF02000011; EEW93876.1; -; Genomic_DNA.
DR RefSeq; WP_006703031.1; NZ_KI391971.1.
DR AlphaFoldDB; D0BLC3; -.
DR STRING; 626369.HMPREF0446_00758; -.
DR eggNOG; COG0768; Bacteria.
DR HOGENOM; CLU_009289_6_1_9; -.
DR OrthoDB; 9804124at2; -.
DR Proteomes; UP000002939; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR Gene3D; 2.20.70.70; -; 1.
DR Gene3D; 3.30.70.2110; -; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR Gene3D; 3.90.1310.10; Penicillin-binding protein 2a (Domain 2); 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR005311; PBP_dimer.
DR InterPro; IPR036138; PBP_dimer_sf.
DR InterPro; IPR001460; PCN-bd_Tpept.
DR PANTHER; PTHR30627; PEPTIDOGLYCAN D,D-TRANSPEPTIDASE; 1.
DR PANTHER; PTHR30627:SF1; PEPTIDOGLYCAN D,D-TRANSPEPTIDASE FTSI; 1.
DR Pfam; PF03717; PBP_dimer; 1.
DR Pfam; PF00905; Transpeptidase; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR SUPFAM; SSF56519; Penicillin binding protein dimerisation domain; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000002939};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 15..36
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 64..232
FT /note="Penicillin-binding protein dimerisation"
FT /evidence="ECO:0000259|Pfam:PF03717"
FT DOMAIN 276..586
FT /note="Penicillin-binding protein transpeptidase"
FT /evidence="ECO:0000259|Pfam:PF00905"
SQ SEQUENCE 596 AA; 66408 MW; 825A2A02546B5ADF CRC64;
MKMNNFKEYL GNRKIAIRCL MAIAFFLFLI FSFHFFKIMV LGQSHGVNLR DELDKKLTHT
TLLKAKRGNI YDASGSPIAV DATNYSLFAV LTDQWDKGKK DYVQDFSKTA QVLSKYIDMK
APEIEKILSQ KNVSQVEFGN AGKNLSVETK EKIEKEELPG IKFTENLSRY YPNGIFASHL
IGYTDVEEQQ VDGKPVEQLV GKMGIEASYN ATLTGKPGEL VSRLDGNGYV IPGSEKITEQ
PKDGNDLYLT LDKKMQTYLE SLVSQVTEKY LPNQLTAMLV DPKTGNIVAA TQRPTFNGTT
KEGISTMWNN LLMDEAYEPG STMKVLALAA AINEGVFDPN EKSESGKIQI FKDLVRDYNK
VGWGRITYLE GLAHSSNVAF VHVVQKIGVE KWKQYLDLFG FGQSTQSGFL NEATGLNTYN
SYLQQLSTGF GQGISVTAFQ MVQAFTAIAN QGEMKKLRIV DHEFRPATNT TVNFSENSLG
QVIKPETAKK TLEYLRQATT MKDGTSNGFD IEGEEIASKT GTAEFVNPET GTYYSYGSNY
IYSVVGFAPA SDPKYILYIT LKQPQNNPYG AGGSALIKEI FNPMMKRALD YSRLTQ
//
