ID D0IF05_9VIBR Unreviewed; 355 AA.
AC D0IF05;
DT 15-DEC-2009, integrated into UniProtKB/TrEMBL.
DT 15-DEC-2009, sequence version 1.
DT 27-MAR-2024, entry version 49.
DE RecName: Full=Alkanal monooxygenase {ECO:0000256|RuleBase:RU367112};
DE EC=1.14.14.3 {ECO:0000256|RuleBase:RU367112};
DE AltName: Full=Bacterial luciferase {ECO:0000256|RuleBase:RU367112};
GN ORFNames=VOA_000208 {ECO:0000313|EMBL:EEZ00158.1};
OS Vibrio sp. RC586.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=675815 {ECO:0000313|EMBL:EEZ00158.1, ECO:0000313|Proteomes:UP000006226};
RN [1] {ECO:0000313|EMBL:EEZ00158.1, ECO:0000313|Proteomes:UP000006226}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RC586 {ECO:0000313|EMBL:EEZ00158.1,
RC ECO:0000313|Proteomes:UP000006226};
RG Los Alamos National Laboratory (LANL);
RG National Microbial Pathogen Data Resource (NMPDR);
RA Chertkov O., Munk A.C., Tapia R., Green L., Rogers Y., Detter J.C.,
RA Bruce D., Brettin T.S., Colwell R.R., Huq A., Grim C.J., Hasan N.A.,
RA Bartels D., Vonstein V.;
RL Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Light-emitting reaction in luminous bacteria.
CC {ECO:0000256|RuleBase:RU367112}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a long-chain fatty aldehyde + FMNH2 + O2 = a long-chain fatty
CC acid + FMN + 2 H(+) + H2O + hnu; Xref=Rhea:RHEA:17181,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:17176, ChEBI:CHEBI:30212, ChEBI:CHEBI:57560,
CC ChEBI:CHEBI:57618, ChEBI:CHEBI:58210; EC=1.14.14.3;
CC Evidence={ECO:0000256|ARBA:ARBA00001552,
CC ECO:0000256|RuleBase:RU367112};
CC -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC {ECO:0000256|ARBA:ARBA00011870, ECO:0000256|RuleBase:RU367112}.
CC -!- SIMILARITY: Belongs to the bacterial luciferase oxidoreductase family.
CC {ECO:0000256|ARBA:ARBA00010426, ECO:0000256|RuleBase:RU367112}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; ADBD01000007; EEZ00158.1; -; Genomic_DNA.
DR RefSeq; WP_000668386.1; NZ_ADBD01000007.1.
DR AlphaFoldDB; D0IF05; -.
DR eggNOG; COG2141; Bacteria.
DR OrthoDB; 7903015at2; -.
DR Proteomes; UP000006226; Unassembled WGS sequence.
DR GO; GO:0047646; F:alkanal monooxygenase (FMN-linked) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-UniRule.
DR CDD; cd01096; Alkanal_monooxygenase; 1.
DR Gene3D; 3.20.20.30; Luciferase-like domain; 1.
DR InterPro; IPR033924; Alkanal_monooxygenase.
DR InterPro; IPR018235; Bacterial_luciferase_CS.
DR InterPro; IPR011251; Luciferase-like_dom.
DR InterPro; IPR036661; Luciferase-like_sf.
DR InterPro; IPR002103; Luciferase_bac/NFP.
DR PANTHER; PTHR30137; LUCIFERASE-LIKE MONOOXYGENASE; 1.
DR PANTHER; PTHR30137:SF8; OXIDOREDUCTASE-RELATED; 1.
DR Pfam; PF00296; Bac_luciferase; 1.
DR PRINTS; PR00089; LUCIFERASE.
DR SUPFAM; SSF51679; Bacterial luciferase-like; 1.
DR PROSITE; PS00494; BACTERIAL_LUCIFERASE; 1.
PE 3: Inferred from homology;
KW Flavoprotein {ECO:0000256|RuleBase:RU367112};
KW FMN {ECO:0000256|RuleBase:RU367112};
KW Luminescence {ECO:0000256|ARBA:ARBA00023223,
KW ECO:0000256|RuleBase:RU367112};
KW Monooxygenase {ECO:0000256|RuleBase:RU367112};
KW Oxidoreductase {ECO:0000256|RuleBase:RU367112,
KW ECO:0000313|EMBL:EEZ00158.1};
KW Photoprotein {ECO:0000256|ARBA:ARBA00023262,
KW ECO:0000256|RuleBase:RU367112}.
FT DOMAIN 1..321
FT /note="Luciferase-like"
FT /evidence="ECO:0000259|Pfam:PF00296"
SQ SEQUENCE 355 AA; 40273 MW; A742CED7066F9FDC CRC64;
MKFGNFLLTY QPPELSQTEV MQRLVNLGRA SESCGFEAVW LLEHHFTEFG LLGNPYVAAA
NLLGATKHLH VGTAAIVLPT AHPIRQLEDV NLLDQLSKGR FRFGICRGLY DKDFRVFGTD
MNNSRALMDC WYDLITTGMT QGSVSADNEH IHFPEVKVQP APYHRSGAPV YVVAESASTT
EWAAKRGLPM ILSWIINTHE KKAQLDLYNE IALEHGHDIQ NIDHCLSYIT SVDHDSQRAK
DICRQFLAHW YDSYVNATRI FDDSDQTKGY DFNKGQWRDF VLKGHRDTNR RIDYSYEINP
VGTPEECIRI IQQDIDATGI TNICCGFEAN GSEQEIIASM KLFQAEVMPH LKSTN
//
