ID D0KYM7_HALNC Unreviewed; 223 AA.
AC D0KYM7;
DT 15-DEC-2009, integrated into UniProtKB/TrEMBL.
DT 15-DEC-2009, sequence version 1.
DT 27-MAR-2024, entry version 52.
DE RecName: Full=tRNA (guanine(46)-N(7))-methyltransferase {ECO:0000256|ARBA:ARBA00011977};
DE EC=2.1.1.33 {ECO:0000256|ARBA:ARBA00011977};
GN OrderedLocusNames=Hneap_0699 {ECO:0000313|EMBL:ACX95550.1};
OS Halothiobacillus neapolitanus (strain ATCC 23641 / c2) (Thiobacillus
OS neapolitanus).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Chromatiales;
OC Halothiobacillaceae; Halothiobacillus.
OX NCBI_TaxID=555778 {ECO:0000313|EMBL:ACX95550.1, ECO:0000313|Proteomes:UP000009102};
RN [1] {ECO:0000313|EMBL:ACX95550.1, ECO:0000313|Proteomes:UP000009102}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 23641 / c2 {ECO:0000313|Proteomes:UP000009102};
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D.,
RA Goodwin L., Pitluck S., Davenport K., Brettin T., Detter J.C., Han C.,
RA Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.,
RA Kerfeld C., Cannon G., Heinhort S.;
RT "Complete sequence of Halothiobacillus neapolitanus c2.";
RL Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the formation of N(7)-methylguanine at position 46
CC (m7G46) in tRNA. {ECO:0000256|ARBA:ARBA00003015}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-
CC methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:42708, Rhea:RHEA-COMP:10188, Rhea:RHEA-COMP:10189,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269,
CC ChEBI:CHEBI:74480; EC=2.1.1.33;
CC Evidence={ECO:0000256|ARBA:ARBA00000142};
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DR EMBL; CP001801; ACX95550.1; -; Genomic_DNA.
DR RefSeq; WP_012823586.1; NC_013422.1.
DR AlphaFoldDB; D0KYM7; -.
DR STRING; 555778.Hneap_0699; -.
DR KEGG; hna:Hneap_0699; -.
DR eggNOG; COG0220; Bacteria.
DR HOGENOM; CLU_078981_0_0_6; -.
DR OrthoDB; 9809889at2; -.
DR Proteomes; UP000009102; Chromosome.
DR GO; GO:0008176; F:tRNA (guanine(46)-N7)-methyltransferase activity; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR003358; tRNA_(Gua-N-7)_MeTrfase_Trmb.
DR Pfam; PF02390; Methyltransf_4; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51625; SAM_MT_TRMB; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000313|EMBL:ACX95550.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000009102};
KW Transferase {ECO:0000313|EMBL:ACX95550.1}.
SQ SEQUENCE 223 AA; 25624 MW; 491C1922266D416D CRC64;
MHSNHTVSGN SRAVISTQTG PHEQLLAQVT KHSRSDWGKP ITDYNRAAFD RAQEWLLERT
GKLILDSGCG TGDSSRALAE MFPAHQIIGI DRSAHRLSRQ RQHTPDNCLL VRADLLDFWR
LAEQAHWRPE RHFLLYPNPE PKPKHLTRRF HAHPIFPVLL ALGGAFECRS NWLIYLEEMA
LALQYHHKTA QIQPIDGNVP PCSPFEHKYR ISGQPLWRLQ AML
//
