ID D0UZI5_9NOCA Unreviewed; 415 AA.
AC D0UZI5;
DT 15-DEC-2009, integrated into UniProtKB/TrEMBL.
DT 15-DEC-2009, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
DE Flags: Fragment;
OS Nocardia carnea.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC Nocardia.
OX NCBI_TaxID=37328 {ECO:0000313|EMBL:ACX70140.1};
RN [1] {ECO:0000313|EMBL:ACX70140.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=W8368 {ECO:0000313|EMBL:ACX70140.1};
RA Moser B.D., Lasker B.A., Steigerwalt A.G., Hinrikson H.P., Brown J.M.;
RT "Nocardia niwae, sp. nov., isolated from human pulmonary sources.";
RL Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrB family.
CC {ECO:0000256|ARBA:ARBA00010708}.
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DR EMBL; GQ984361; ACX70140.1; -; Genomic_DNA.
DR AlphaFoldDB; D0UZI5; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0034335; F:DNA negative supercoiling activity; IEA:UniProt.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00822; TopoII_Trans_DNA_gyrase; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.40.50.670; -; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR014721; Ribsml_uS5_D2-typ_fold_subgr.
DR InterPro; IPR001241; Topo_IIA.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR000565; Topo_IIA_B.
DR InterPro; IPR013759; Topo_IIA_B_C.
DR InterPro; IPR013506; Topo_IIA_bsu_dom2.
DR InterPro; IPR018522; TopoIIA_CS.
DR InterPro; IPR006171; TOPRIM_domain.
DR PANTHER; PTHR45866:SF1; DNA GYRASE SUBUNIT B, MITOCHONDRIAL; 1.
DR PANTHER; PTHR45866; DNA GYRASE/TOPOISOMERASE SUBUNIT B; 1.
DR Pfam; PF00204; DNA_gyraseB; 1.
DR Pfam; PF01751; Toprim; 1.
DR PRINTS; PR01159; DNAGYRASEB.
DR PRINTS; PR00418; TPI2FAMILY.
DR SMART; SM00433; TOP2c; 1.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1.
DR SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
DR PROSITE; PS00177; TOPOISOMERASE_II; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 342..415
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ACX70140.1"
FT NON_TER 415
FT /evidence="ECO:0000313|EMBL:ACX70140.1"
SQ SEQUENCE 415 AA; 45374 MW; DAE70D1906BD5605 CRC64;
SDSYAVSGGL HGVGISVVNA LSSRLEAEVD TDGYHWSQTY KDAVPGPLVQ GEPTKATGTT
IRFWADPAIF ETTTYNFETI SRRLQEMAFL NKGLTIVLTD ERVSATEVTD EMVSETAEAP
KHDEGGEKPA VEHKVKARTY HYPGGLEDFV RHINRTKQPI HNSAVSFNGK GPGHELEVAM
QWNSGYSESV HTFANTINTH EGGTHEEGFR AALTTVVNRY AKEKKLLKDK DGNLTGDDIR
EGLAAIVSVK VSEPQFEGQT KTKLGNTEVK SFVQRACNEH LAHWFEANPA DAKTIVNKAV
SSAQARLAAR KARELVRRKS ATDIGGLPGK LADCRSKDPS KSEIYIVEGD SAGGSAKSGR
DSMYQAILPL RGKIINVEKA RIDKVLKNAE VQAIITAFGT GIHDEFDIAK LRYHK
//
