ID D2IE21_9PICO Unreviewed; 2177 AA.
AC D2IE21;
DT 09-FEB-2010, integrated into UniProtKB/TrEMBL.
DT 09-FEB-2010, sequence version 1.
DT 27-MAR-2024, entry version 85.
DE RecName: Full=Genome polyprotein {ECO:0000256|ARBA:ARBA00020107};
OS Human parechovirus 3.
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Picornavirales; Picornaviridae; Paavivirinae; Parechovirus; Parechovirus A.
OX NCBI_TaxID=195055 {ECO:0000313|EMBL:ADA79691.1, ECO:0000313|Proteomes:UP000145574};
RN [1] {ECO:0000313|EMBL:ADA79691.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=K11-94 {ECO:0000313|EMBL:ADA79691.1}, and K20-94
RC {ECO:0000313|EMBL:ADA79693.1};
RA Benschop K.S.M., de Vries M., Minnaar R.P., Stanway G., van der Hoek L.,
RA Wolthers K.C., Simmonds P.;
RL Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000100453, ECO:0000313|Proteomes:UP000145574}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K11-94 {ECO:0000313|EMBL:ADA79691.1}, and K20-94
RC {ECO:0000313|EMBL:ADA79693.1};
RX PubMed=19759239; DOI=10.1099/vir.0.014670-0;
RA Benschop K.S., de Vries M., Minnaar R.P., Stanway G., van der Hoek L.,
RA Wolthers K.C., Simmonds P.;
RT "Comprehensive full-length sequence analyses of human parechoviruses:
RT diversity and recombination.";
RL J. Gen. Virol. 91:145-154(2010).
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane
CC {ECO:0000256|ARBA:ARBA00004180}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004180}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004180}. Host cytoplasmic vesicle membrane
CC {ECO:0000256|ARBA:ARBA00004295}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004295}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004295}. Membrane
CC {ECO:0000256|ARBA:ARBA00004287}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004287}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004287}.
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DR EMBL; GQ183030; ADA79691.1; -; Genomic_RNA.
DR EMBL; GQ183032; ADA79693.1; -; Genomic_RNA.
DR Proteomes; UP000100453; Genome.
DR Proteomes; UP000145574; Genome.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044162; C:host cell cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0039618; C:T=pseudo3 icosahedral viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0005216; F:monoatomic ion channel activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0051259; P:protein complex oligomerization; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR GO; GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-KW.
DR GO; GO:0039707; P:virus-mediated pore formation in host cell membrane; IEA:UniProtKB-KW.
DR CDD; cd23217; Parechovirus_RdRp; 1.
DR CDD; cd00205; rhv_like; 2.
DR Gene3D; 2.60.120.20; -; 3.
DR Gene3D; 3.30.70.270; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.10.10; Trypsin-like serine proteases; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR004004; Helic/Pol/Pept_Calicivir-typ.
DR InterPro; IPR000605; Helicase_SF3_ssDNA/RNA_vir.
DR InterPro; IPR014759; Helicase_SF3_ssRNA_vir.
DR InterPro; IPR007053; LRAT_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR044067; PCV_3C_PRO.
DR InterPro; IPR000199; Peptidase_C3A/C3B_picornavir.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001676; Picornavirus_capsid.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR033703; Rhv-like.
DR InterPro; IPR001205; RNA-dir_pol_C.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR InterPro; IPR029053; Viral_coat.
DR InterPro; IPR009419; VPP_parechovir_P3A.
DR InterPro; IPR009407; VPP_parechovir_P3B.
DR Pfam; PF06344; Parecho_VpG; 1.
DR Pfam; PF00548; Peptidase_C3; 1.
DR Pfam; PF06363; Picorna_P3A; 1.
DR Pfam; PF00680; RdRP_1; 1.
DR Pfam; PF00073; Rhv; 1.
DR Pfam; PF00910; RNA_helicase; 1.
DR PRINTS; PR00918; CALICVIRUSNS.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF88633; Positive stranded ssRNA viruses; 3.
DR SUPFAM; SSF50494; Trypsin-like serine proteases; 1.
DR PROSITE; PS51934; LRAT; 1.
DR PROSITE; PS51874; PCV_3C_PRO; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
DR PROSITE; PS51218; SF3_HELICASE_2; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Capsid protein {ECO:0000256|ARBA:ARBA00022561};
KW Covalent protein-RNA linkage {ECO:0000256|ARBA:ARBA00022520};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW Host cytoplasmic vesicle {ECO:0000256|ARBA:ARBA00022488};
KW Host membrane {ECO:0000256|ARBA:ARBA00022870};
KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Ion channel {ECO:0000256|ARBA:ARBA00023303};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484};
KW T=pseudo3 icosahedral capsid protein {ECO:0000256|ARBA:ARBA00022706};
KW Thiol protease {ECO:0000256|ARBA:ARBA00022807};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transport {ECO:0000256|ARBA:ARBA00022448};
KW Viral attachment to host cell {ECO:0000256|ARBA:ARBA00022804};
KW Viral ion channel {ECO:0000256|ARBA:ARBA00023039};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Virion {ECO:0000256|ARBA:ARBA00022706};
KW Virus entry into host cell {ECO:0000256|ARBA:ARBA00023296}.
FT DOMAIN 784..878
FT /note="LRAT"
FT /evidence="ECO:0000259|PROSITE:PS51934"
FT DOMAIN 1154..1315
FT /note="SF3 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51218"
FT DOMAIN 1515..1705
FT /note="Peptidase C3"
FT /evidence="ECO:0000259|PROSITE:PS51874"
FT DOMAIN 1942..2056
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50507"
SQ SEQUENCE 2177 AA; 245993 MW; 5734874438C50AC2 CRC64;
MESIKDLVNV ATGAMDTLSL SNVEAEANNI ISGNEVGGEI ITKVADDASN LLGPNSFATT
AQPENKDVVQ ATTTVNTTNL TQHPSAPTIP FTPDFRNVDN FHSMAYDITT GDKNPSKLIR
LDTASWQTSY SRQYKITTVE LPKSFWDDTR KPAYGQAKYF AAVRCGFHFQ VQVNVNQGTA
GSALVVYEPK PVIDSRQYLE FGSLTNLPHV LMNLAETTQA DLCIPYVADT NYVKTDSSDL
GQLRVYVWTP LSVPTGASNE VDVTVMGSLL QLDFQNPRPY GEDVEIYDNG PNKVNASKFN
KRKFLTASTK YKWTRTKVDI AEGPGTMNMA NVLSTTGAQS VALVGERAFY DPRTAGSKSR
FDDMIKIAQL FSVMSDNTTP SSSSGIDKYG YFDWAATVAP QNMVHRNVVT LDQFPNLNLF
MNTYSYFRGS LIIRLSIYAS TFNRGRLRMG FFPNCTHDTQ LELDNAIYTI CDIGSDNSFE
LTIPYSFSTW MRKTHGHQLG LFQVEVLNRL TYNSSSPNKV HCIVQGRLGD DAKFFCPTGS
LVSFQNSWGS QMDLTDPLCI EDNMENCKQS ISPNELGLTS AQDDGPLGNE KPNYFLNFRT
MNVDIFTVSH TKVDNIFGRA WYVTSHDFNN GDTWRQKLTF PKEGHGMLSQ FFAYFTGEIN
IHILYMAEQG FLRVAHTYDT EDNRKTFLSS NGVITIPAGE QMTLSVPFYS NKPLRTVRHD
SALGFLMCRP MMHGTTRTTA EVYVSLRCPN FFFPVPAPKP TGSRATALYD ESPYVPTDNR
KMQLAYLDRG FYKHYGIIVG ENVYQLDSDD IFKTALTGKA RFTKTKLTPD WVIEEECELD
YFRVKYLESS VNSEHIFSVD HNCETIAKDI FGTHTLNQHQ AIGLVGTILL TAGLMSTIKT
PVNATTIKEF FNHAVEGDEQ GLSLLVQKCT TFFSSAATEI LDNDLVKFIV KILVRILCYM
VLYCHKPNIL TTACLSTLLI MDVTSSSVLS PSCKALMQCL MDGDVKKLAE VVAESMSNTD
DEEIKEQICD TVKYTKSILS NQGPFKGFNE VSTAFRHIDW WIHTLLKIKD MVLSVFKPSM
ESKAIQWLER NKEHVCAILD YASDIIVESK DQTKMKSQEF YQRYTDCLSK FKPIMAICFR
SCHNSISNTV YRLFQELARI PSRISTQNDL IRVEPIGVWI QGEPGQGKSF LTHTLSRQLQ
KSCKLNGVYT NPTASEFMDG YDNQDIHLID DLGQTRKEKD IEMLCNCISS VPFIVPMAHL
EEKGKFYTSK LVIATTNKSD FSSTVLQDSG ALKRRFPYIM HIRAAKAYSK AGKLNVSQAM
STMATGECWE VSKNGRDWET LKLKDLVQKI TEDYQERQKN YDCWKQQLEN QTLDDLDDAV
SYIKHNFPDA IPYIDEYLNI EMSTLIEQME AFIEPRPSVF KCFATRVANQ TRKAAKEVVE
WFSSKIKSML SFVERNKAWL TVASAVTSAI SILLLVTKIF KKEDSKDERA YNPTLPMAKP
KGTFPVSQRE FKNEAPYDGQ LEHIISQMAY ITGSTTGHLT HCAGYQHDEI ILHGHSIKYL
EQEEDLTLHY KNKVFTIENP SVTQVTLGGK PMDLAILKCK LPFRFKKNSK YYTNKIGTES
MLIWMTEQGI ITKEVRRVHH SGGIKTREGT ESTKTISYTV KSCKGMCGGL LISKVEGNFK
ILGMHIAGNG EMGVAIPFNF LKNDMSDQGI VTEITPMQPM YINTKTQIHK SPVYGAVEVK
MGPAVLSKSD PRLEEPVECL IKKSAAKYRV NKFQVNNELW QGVKACVKSK FREIFGINGI
VDMKTAILGT SHVNSMDLST SAGYNFVKSG YKKKDLICLE PFSVSPMLEK LVQDKFHALL
KGNQITTVFN TCLKDELRKL DKISAGKTRC IEACEVDYCI VYRMIMMEIY DKIYQTPCYY
SGLAVGINPY KDWHFMINAL NDYNYEMDYS QYDGSLSSML LWEAVEVLAY CHDSPDLVMQ
LHKPVIDSDH VVFNERWLIH GGMPSGSPCT TVLNSLCNLM MCIYTTNLIS PGIDCLPIVY
GDDVILSLDR EIDPEKLQGI MADSFGAEVT GSRKDEPPSL KPRLEVEFLK RKPGYFPEST
FIVGKLDTEN MIQHLMWMKN FSTFKQQLQS YLMELCLHGK DTYLYYIKIL DPYLKEWNIT
VDDYDVVIAK LMPMVFD
//
