ID   D2VS80_NAEGR            Unreviewed;       482 AA.
AC   D2VS80;
DT   02-MAR-2010, integrated into UniProtKB/TrEMBL.
DT   02-MAR-2010, sequence version 1.
DT   27-MAR-2024, entry version 57.
DE   SubName: Full=Predicted protein {ECO:0000313|EMBL:EFC40379.1};
GN   ORFNames=NAEGRDRAFT_71844 {ECO:0000313|EMBL:EFC40379.1};
OS   Naegleria gruberi (Amoeba).
OC   Eukaryota; Discoba; Heterolobosea; Tetramitia; Eutetramitia;
OC   Vahlkampfiidae; Naegleria.
OX   NCBI_TaxID=5762 {ECO:0000313|Proteomes:UP000006671};
RN   [1] {ECO:0000313|EMBL:EFC40379.1, ECO:0000313|Proteomes:UP000006671}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NEG-M {ECO:0000313|EMBL:EFC40379.1,
RC   ECO:0000313|Proteomes:UP000006671};
RX   PubMed=20211133; DOI=10.1016/j.cell.2010.01.032;
RA   Fritz-Laylin L.K., Prochnik S.E., Ginger M.L., Dacks J.B., Carpenter M.L.,
RA   Field M.C., Kuo A., Paredez A., Chapman J., Pham J., Shu S., Neupane R.,
RA   Cipriano M., Mancuso J., Tu H., Salamov A., Lindquist E., Shapiro H.,
RA   Lucas S., Grigoriev I.V., Cande W.Z., Fulton C., Rokhsar D.S., Dawson S.C.;
RT   "The genome of Naegleria gruberi illuminates early eukaryotic
RT   versatility.";
RL   Cell 140:631-642(2010).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR   EMBL; GG738893; EFC40379.1; -; Genomic_DNA.
DR   RefSeq; XP_002673123.1; XM_002673077.1.
DR   AlphaFoldDB; D2VS80; -.
DR   STRING; 5762.D2VS80; -.
DR   EnsemblProtists; EFC40379; EFC40379; NAEGRDRAFT_71844.
DR   GeneID; 8854810; -.
DR   VEuPathDB; AmoebaDB:NAEGRDRAFT_71844; -.
DR   eggNOG; ENOG502QV91; Eukaryota.
DR   InParanoid; D2VS80; -.
DR   OMA; THWILRR; -.
DR   OrthoDB; 276083at2759; -.
DR   Proteomes; UP000006671; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0070475; P:rRNA base methylation; IEA:InterPro.
DR   GO; GO:0030488; P:tRNA methylation; IEA:InterPro.
DR   CDD; cd01335; Radical_SAM; 1.
DR   Gene3D; 1.10.150.530; -; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR040072; Methyltransferase_A.
DR   InterPro; IPR027492; RNA_MTrfase_RlmN.
DR   InterPro; IPR004383; rRNA_lsu_MTrfase_RlmN/Cfr.
DR   InterPro; IPR007197; rSAM.
DR   NCBIfam; TIGR00048; rRNA_mod_RlmN; 1.
DR   PANTHER; PTHR30544; 23S RRNA METHYLTRANSFERASE; 1.
DR   PANTHER; PTHR30544:SF5; RADICAL SAM CORE DOMAIN-CONTAINING PROTEIN; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDF00275; adenosine_C2_methyltransferase; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   4: Predicted;
KW   4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006671};
KW   rRNA processing {ECO:0000256|ARBA:ARBA00022552};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          192..438
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000259|PROSITE:PS51918"
SQ   SEQUENCE   482 AA;  54448 MW;  79A34B99BD1D7280 CRC64;
     MQKLILGNLC ARQIVINGNF QHRFYSTQIV RKSLMGLNKT EMKEILEKTH PHVASSFRLN
     EIYKFIYKFG ARKFDDITVL TKSDRQSLSE LYSIDILGNI EEEIKSKKDK HTRKFLFAFQ
     NPKYVPPVAD NSVDSTIVST TSSASSSCGN QQNISDADVA ISPMKKPTLK EQKQFNKVEA
     VYIYHPPKAS DSFGRGTVCL SSQVGCSLNC KFCRTGTAPI ERNLLASEIV SQLVSVKHRL
     MDFPIYMTEE ERKRASIEKS FVNNIVFMGE GEPLYNYKNV KKAIEILSDG CGISKRKIIV
     STSGVCNLIP DVVNDLGVNL AISLHATTNE LRDNIVPINK IFPLEVLFET LREQCFKNNN
     RHITFEYVML NHVNDFIDDA KRLVKLVKDI PCSVNLIAFN EWEGSGFECS SDERIEEFSK
     YLYKNGISAP VRHSKGQDIL GACGQLKNRN ENKAGALQQT EQNVATTVEM IRRNSKQPSS
     KK
//