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Database: UniProt
Entry: D3E050_METRM
LinkDB: D3E050_METRM
Original site: D3E050_METRM 
ID   D3E050_METRM            Unreviewed;       550 AA.
AC   D3E050;
DT   23-MAR-2010, integrated into UniProtKB/TrEMBL.
DT   23-MAR-2010, sequence version 1.
DT   25-OCT-2017, entry version 37.
DE   RecName: Full=Methyl-coenzyme M reductase subunit alpha {ECO:0000256|PIRNR:PIRNR000262};
DE            EC=2.8.4.1 {ECO:0000256|PIRNR:PIRNR000262};
GN   Name=mcrA {ECO:0000313|EMBL:ADC47774.1};
GN   OrderedLocusNames=mru_1924 {ECO:0000313|EMBL:ADC47774.1};
OS   Methanobrevibacter ruminantium (strain ATCC 35063 / DSM 1093 / JCM
OS   13430 / OCM 146 / M1) (Methanobacterium ruminantium).
OC   Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales;
OC   Methanobacteriaceae; Methanobrevibacter.
OX   NCBI_TaxID=634498 {ECO:0000313|EMBL:ADC47774.1, ECO:0000313|Proteomes:UP000008680};
RN   [1] {ECO:0000313|EMBL:ADC47774.1, ECO:0000313|Proteomes:UP000008680}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35063 / DSM 1093 / JCM 13430 / OCM 146 / M1
RC   {ECO:0000313|Proteomes:UP000008680};
RX   PubMed=20126622; DOI=10.1371/journal.pone.0008926;
RA   Leahy S.C., Kelly W.J., Altermann E., Ronimus R.S., Yeoman C.J.,
RA   Pacheco D.M., Li D., Kong Z., McTavish S., Sang C., Lambie S.C.,
RA   Janssen P.H., Dey D., Attwood G.T.;
RT   "The genome sequence of the rumen methanogen Methanobrevibacter
RT   ruminantium reveals new possibilities for controlling ruminant methane
RT   emissions.";
RL   PLoS ONE 5:E8926-E8926(2010).
CC   -!- FUNCTION: Reduction of methyl-coenzyme M (2-(methylthio)
CC       ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate
CC       to methane and a heterodisulfide. {ECO:0000256|PIRNR:PIRNR000262}.
CC   -!- CATALYTIC ACTIVITY: Methyl-CoM + CoB = CoM-S-S-CoB + methane.
CC       {ECO:0000256|PIRNR:PIRNR000262}.
CC   -!- COFACTOR:
CC       Name=coenzyme F430; Xref=ChEBI:CHEBI:60540;
CC         Evidence={ECO:0000256|PIRNR:PIRNR000262};
CC       Note=Binds 1 coenzyme F430 noncovalently per subunit. Coenzyme
CC       F430 is a yellow nickel porphinoid.
CC       {ECO:0000256|PIRNR:PIRNR000262};
CC   -!- PATHWAY: One-carbon metabolism; methyl-coenzyme M reduction;
CC       methane from methyl-coenzyme M: step 1/1.
CC       {ECO:0000256|PIRNR:PIRNR000262}.
CC   -!- SUBUNIT: Hexamer of two alpha, two beta, and two gamma chains.
CC       {ECO:0000256|PIRNR:PIRNR000262}.
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DR   EMBL; CP001719; ADC47774.1; -; Genomic_DNA.
DR   RefSeq; WP_012956722.1; NC_013790.1.
DR   ProteinModelPortal; D3E050; -.
DR   STRING; 634498.mru_1924; -.
DR   EnsemblBacteria; ADC47774; ADC47774; mru_1924.
DR   GeneID; 8771594; -.
DR   KEGG; mru:mru_1924; -.
DR   PATRIC; fig|634498.28.peg.1924; -.
DR   eggNOG; arCOG04857; Archaea.
DR   eggNOG; COG4058; LUCA.
DR   HOGENOM; HOG000225809; -.
DR   KO; K00399; -.
DR   OMA; GRVCDGG; -.
DR   OrthoDB; POG093Z00ZI; -.
DR   UniPathway; UPA00646; UER00699.
DR   Proteomes; UP000008680; Chromosome.
DR   GO; GO:0050524; F:coenzyme-B sulfoethylthiotransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0015948; P:methanogenesis; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.840.10; -; 1.
DR   Gene3D; 3.30.70.470; -; 1.
DR   Gene3D; 3.90.390.10; -; 1.
DR   InterPro; IPR016212; Me_CoM_Rdtase_asu.
DR   InterPro; IPR008924; Me_CoM_Rdtase_asu/bsu_C.
DR   InterPro; IPR009047; Me_CoM_Rdtase_asu_C.
DR   InterPro; IPR003183; Me_CoM_Rdtase_asu_N.
DR   InterPro; IPR015811; Me_CoM_Rdtase_asu_N_sub1.
DR   InterPro; IPR015823; Me_CoM_Rdtase_asu_N_sub2.
DR   InterPro; IPR009024; Me_CoM_Rdtase_Fd-like_fold.
DR   Pfam; PF02249; MCR_alpha; 1.
DR   Pfam; PF02745; MCR_alpha_N; 1.
DR   PIRSF; PIRSF000262; MCR_alpha; 1.
DR   SUPFAM; SSF48081; SSF48081; 1.
DR   SUPFAM; SSF55088; SSF55088; 1.
DR   TIGRFAMs; TIGR03256; met_CoM_red_alp; 1.
PE   4: Predicted;
KW   Complete proteome {ECO:0000313|Proteomes:UP000008680};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR000262,
KW   ECO:0000256|PIRSR:PIRSR000262-1};
KW   Methanogenesis {ECO:0000256|PIRNR:PIRNR000262};
KW   Nickel {ECO:0000256|PIRNR:PIRNR000262, ECO:0000256|PIRSR:PIRSR000262-
KW   1}; Reference proteome {ECO:0000313|Proteomes:UP000008680};
KW   Transferase {ECO:0000256|PIRNR:PIRNR000262,
KW   ECO:0000313|EMBL:ADC47774.1}.
FT   DOMAIN        3    268       MCR_alpha_N. {ECO:0000259|Pfam:PF02745}.
FT   DOMAIN      315    441       MCR_alpha. {ECO:0000259|Pfam:PF02249}.
FT   METAL       147    147       Nickel. {ECO:0000256|PIRSR:PIRSR000262-
FT                                1}.
FT   MOD_RES     257    257       Pros-methylhistidine. {ECO:0000256|PIRSR:
FT                                PIRSR000262-2}.
FT   MOD_RES     271    271       5-methylarginine. {ECO:0000256|PIRSR:
FT                                PIRSR000262-2}.
SQ   SEQUENCE   550 AA;  59780 MW;  E9DCD8215EB90785 CRC64;
     MADKKFLDAM TKKFKEAPEE KTTTFYNMGG WTQSERKTEF VNEGKAIAEA RGIPMYNPDI
     GNPLGQRALM SYQLSGTDTF VEGDDLHFIN NAAMQQAWDD IRKTVIVGLN TAHNVLEKRL
     GMEVTPETIT NYLEVVNHAM PGAAAVQEHM VETNPLLVDD SYVKVFTGDD DLAAEIDPAF
     VLDINKEFPE EQAEALKAEV GGAIWQIVRV PSVVGRVCDG GTTSRWSAMQ IGMSMISAYG
     QCAGEGATGD FAYASKHAEV IGMGTYLPIR RARAGNELGG VPFGFMADIC QATRVTDDPV
     ESALEVVALG AALYDQIWLG SYMSGGVGFT QYATAAYTDN VLDDFSYFGK DYVEDKYGDL
     CSAPNDMDTV LDVGSAVTFY SLEQYEEYPA LLETHFGGSQ RAAVVSAASG ISTAFATGNA
     QTGLSAWYLA QYLHKEQHSR LGFYGYDLQD QCGAANVFAI RNDEGLPLEL RGPNYPNYAM
     NVGHQGEYAG IAQAPHSARG DAFAVNPLVK IAFADKNLPF DFTKVRAEFA KGALREFEPA
     GERSIIIPAK
//
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