ID D3G7A6_SORCE Unreviewed; 3798 AA.
AC D3G7A6;
DT 23-MAR-2010, integrated into UniProtKB/TrEMBL.
DT 23-MAR-2010, sequence version 1.
DT 27-MAR-2024, entry version 61.
DE SubName: Full=Polyketide synthase {ECO:0000313|EMBL:ACB46196.1};
GN Name=epoD {ECO:0000313|EMBL:ACB46196.1};
OS Sorangium cellulosum So0157-2.
OC Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae; Sorangium.
OX NCBI_TaxID=1254432 {ECO:0000313|EMBL:ACB46196.1};
RN [1] {ECO:0000313|EMBL:ACB46196.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=So0157-2 {ECO:0000313|EMBL:ACB46196.1};
RX PubMed=23549746; DOI=10.1007/s00253-013-4830-0;
RA Zhu L.P., Li Z.F., Sun X., Li S.G., Li Y.Z.;
RT "Characteristics and activity analysis of epothilone operon promoters from
RT Sorangium cellulosum strains in Escherichia coli.";
RL Appl. Microbiol. Biotechnol. 97:6857-6866(2013).
RN [2] {ECO:0000313|EMBL:ACB46196.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=So0157-2 {ECO:0000313|EMBL:ACB46196.1};
RA Li Y.Z., Li Z.F., Xia Z.J., Zhao J.Y., Sun X., Zhao L., Hu W., Liu H.,
RA Wu Z.H., Liu W.F.;
RT "The biosynthetic gene cluster for the epothilones from Sorangium
RT cellulosum So0157-2.";
RL Submitted (MAY-2015) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; EU414841; ACB46196.1; -; Genomic_DNA.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR CDD; cd08955; KR_2_FAS_SDR_x; 2.
DR CDD; cd00833; PKS; 2.
DR Gene3D; 3.30.70.3290; -; 2.
DR Gene3D; 3.40.47.10; -; 2.
DR Gene3D; 1.10.1200.10; ACP-like; 2.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 2.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 2.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR049490; C883_1060-like_KR_N.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR013217; Methyltransf_12.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020803; PKS_MeTfrase.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF37; FATTY ACID SYNTHASE; 1.
DR Pfam; PF00698; Acyl_transf_1; 2.
DR Pfam; PF21394; Beta-ketacyl_N; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 2.
DR Pfam; PF00109; ketoacyl-synt; 2.
DR Pfam; PF02801; Ketoacyl-synt_C; 2.
DR Pfam; PF08659; KR; 2.
DR Pfam; PF08242; Methyltransf_12; 1.
DR Pfam; PF21089; PKS_DH_N; 1.
DR Pfam; PF00550; PP-binding; 2.
DR Pfam; PF14765; PS-DH; 2.
DR SMART; SM00827; PKS_AT; 2.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00822; PKS_KR; 2.
DR SMART; SM00825; PKS_KS; 2.
DR SMART; SM00828; PKS_MT; 1.
DR SMART; SM00823; PKS_PP; 2.
DR SMART; SM01294; PKS_PP_betabranch; 2.
DR SUPFAM; SSF47336; ACP-like; 2.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 2.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 4.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 2.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS50075; CARRIER; 2.
DR PROSITE; PS00606; KS3_1; 2.
DR PROSITE; PS52004; KS3_2; 2.
PE 4: Predicted;
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 34..455
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1428..1503
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1521..1947
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 3667..3745
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
SQ SEQUENCE 3798 AA; 405703 MW; 4F4EF017F482B70F CRC64;
MTDREGQLLE RLREVTLALR KTLNERDTLE LEKTEPIAIV GIGCRFPGGA GTPEAFWELL
DDGRDAIRPL EERWALVGVD PGDDVPRWAG LLTEAIDGFD AAFFGIAPRE ARSLDPQHRL
LLEVAWEGFE DAGIPPRSLV GSRTGVFVGV CATEYLHAAV AHQPREERDA YSTTGNMLSI
AAGRLSYTLG LQGPCLTVDT ACSSSLVAIH LACRSLRARE SDLALAGGVN MLLSPDTMRA
LARTQALSPN GRCQTFDASA NGFVRGEGCG LIVLKRLSDA RRDGDRIWAL IRGSAINQDG
RSTGLTAPNV LAQGALLREA LRNAGVEAEA IGYIETHGAA TSLGDPIEIE ALRAVVGPAR
ADGARCVLGA VKTNLGHLEG AAGVAGLIKA TLSLHHERIP RNLNFRTLNP RIRIEGTALE
LATEPVPWPR TGRTRFAGVS SFGMSGTNAH VVLEEAPAVE PEAAAPERAA ELFVLSAKSV
AALDAQAARL RDHLEKHVEL GLGDVAFSLT TTRSAMEHRL AVAASSREAL RGALSAAAQG
HTPPGAVRGR ASGGSAPKVV FVFPGQGSQW VGMGRKLMAE EPVFRAALEG CDRAIEAEAG
WSLLGELSAD EAASQLGRID VVQPVLFAME VALSALWRSW GVEPEAVVGH SMGEVAAAHV
AGALSLEDAV AIICRRSRLL RRISGQGEMA LVELSLEEAE AALRGHEGRL SVAVSNSPRS
TVLAGEPAAL SEVLAALTAK GVFWRQVKVD VASHSPQVDP LREELVAALG AIRPRAAAVP
MRSTVTGGVI AGPELGASYW AGNLRQPVRF AAAARALLEG GPTLFIEMSP HPILVPPLDE
IQTAVEQGGA AVGSLRRGQD ERATLLEALG TLWASGYPVS WARLFPAGGR RVPLPTYPWQ
HERCWIEVEP EARRLAAADP TKDWFYRTDW PEVPRAAPKS ETAHGSWLLL ADRGGVGEAV
AAALSTRGLS CTVLHASADA STVAEQVSEA ASRRNDWQGV LYLWGLDAVV DAGASADDVS
EATRRATAPV LGLVRFLSAA PHPPRFWVVT RGACTVGGEP EVSLCQAALW GLARVVALEH
PAAWGGLVDL DPQKSPTEIE PLVAELLSPD AEDQLAFRSG RRHAARLVAA PPEGDVAPIS
LSAEGSYLVT GGLGGLGLLV ARWLVERGAR HLVLTSRHGL PERQASGGEQ PPEARARIAA
VEGLEAQGAR VTVAAVDVAE ADPMTALLAA IEPPLRGVVH AAGVFPVRPL AETDEALLES
VLRPKVAGSW LLHRLLRDRP LDLFVLFSSG AAVWGGKGQG AYAAANAFLD GLAHHRRARS
LPALSLAWGL WAEGGMVDAK AHARLSDIGV LPMATGPALS ALERLVKTSA VQRSVTRMDW
TRFAPVYAAR GRRNLLSALV AEDERTASPP VPTANRIWRG LSVAESRSAL YELVRGIAAR
VLGFADPGAL DVGRGFAEQG LDSLMALEIR NRLQRELGER LSATLAFDHP TVERLVAHLL
TDVLKLEDRS DTRHIRSVAA DDDIAIVGAA CRFPGGDESL ETYWRHLAEG MVVSAEVPAD
RWRAADWYDP DPEVPGRTYV AKGAFLRDVR SLDAAFFSIS PREAMSLDPQ QRLLLEVSWE
AIERAGQDPM ALRETATGVF VGMIGSEHAE RVQGLDDDAA LLYGTTGNLL SVAAGRLSFF
LGLHGPTMTV DTACSSSLVA LHLACQSLRL GECDQALAGG SSVLLSPRSF VAASRMRLLS
PDGRCKTFSA AADGFARAEG CAVVVLKQLR DAQRDRDPIL AVVRSTAINH DGPSSGLTVP
SGPAQQALLG QALAQAGVAP AEVDFVECHG TGTALGDPIE VQALGAVYGR GRPAERPLWL
GAVKANLGHL EAAAGLAGVL KVLLALEHEQ IPAQPELDEL NPHIPWAELP VAVVRRAVPW
PRGARPRRAG VSAFGLSGTN AHVVLEEAPT VEPGAAAPER AAELFVLSAK SVAALDAQAA
RLRDHLEKHV ELGLGDVAFS LATTRSAMEH RLAVAASSRE ALRGALSAAA QGHTPPGAVR
GRASGGSAPK VVFVFPGQGS QWVGMGRKLM AEEPVFRAAL EGCDRAIEAE AGWSLLGELS
ADEAASQLER IDVVQPVLFA MEVALSALWR SWGVEPEAVV GHSMGEVAAA HVAGALSLED
AVAIICRRSR LLRRISGQGE MALVELTLEE AEAALRGHEG RLSVAVSNSP RSTVLAGEPA
ALSEVLAALT AKGVFWRQVK VDVASHSPQV DPLREELVAA LGAIRPRAAA VPMRSTVTGG
VIAGPELGAS YWADNLRQPV RFAAAARALL GGGPTLFIEM SPHPILVPPL DEIQTAVEQG
GAAVGSLRRG QDERATLLEA LGTLWASGYP VSWARLFPAG GRRVPLPTYP WQHERYWIED
SVHGSKPSLR LRQLRNGATD HPLLGASLLV SARPGAHLWE QALSDERLSY LSEHRVHGEA
VLPSAAYVEM ALAAGVDLYG TATLVLEQLA LERALAVPSE GGRIVQVALS EEGPGRASFQ
VSSREEAGRS WVRHATGHVC SDQSSAVGAL KEAPWEIQQR CPSVLSSEAL YPLLNEHALD
YGPCFQGVEQ VWLGTGEVLG RVRLPEDMAS SSGAYRIHPA FLDACFQVLT ALLTTPESID
IRRRLTDLHE PDLPRSRAPV NQAVSDTWLW DAALDGGRRQ SASVPVELVL GSFHAKWEVM
ERLAQAYIID TLRIWDVFCA AGERHTIDEL LVRLQISVGY RKVIKRWMDH LVAIGVLVGD
GEHFVSSQPL PEPDLAAVLE EAGRVFADLP VLLEWCKFAG ERLADVLTGK TLALEILFPG
GSFDMAERIY QDSPIARYSN GIVRGVVESA ARVVAPSGMF SILEIGAGTG ATTATVLPVL
LPDRTEYHFT DVSPLFLARA EQKFRDHPFL KYGILDIDQE PAGQGYAHQK FDVIVAANVI
HATRDIRATA KRLLSLLAPG GLLVLVEGTG HPIWFDITTG LIEGWQKYED DLRTDHPLLP
ARTWCDLLRR VGFADAVSLP GDGSPAGILG QHVILSRAPG IAGAACDSSG ESATESPAAR
AVRQEWADGS ADVVHRMALE RMYFHRRPGR QVWVHGRLRT GGDAFTKALA GDLLLFDDTG
QVVAEVQGLR LPQLEASAFA PRDPREEWLY ALEWQRKDPI PEAPAAASSS SAGAWLVLMD
QGGTGAALVS LLEGRGEACV RVIVGTEYAC LAPGLYQVDP AQSDGFHTLL RDAFGEDRIC
RAVVHMWSLD ATAAGERTTG ESLQADQLLG SLSALSLVQA LVRRRWRNMP RLWLLTRAVH
AVGAEDAAAS VAQAPVWGLG RTLALEHPEL RCTLVDVNPA PSPEDAAALA VELGASDRED
QIALRSDGRY VARLVRSSFS GKPATDRGIR ADGSYVITDG MGRVGLSVAQ WMVMQGARHV
VLVDRGGASE ASRDALRSMA EAGAEVQIVE ADVARRDDVA RLLSKIEPSM PPLRGIVYVD
GTFQVDSSML ELDAHRFKEW MYPKVLGAWN LHALTRDRSL DFFVLYSSGT SLLGLPGQGS
RAAGDAFLDA IAHHRCRLGL TAMSINWGLL FEASSPATPN DGGARLEYRG MEGLTLEQGA
AALGRLLAQP RAQVGVMRLN LRQWLDFYPN AARLALWAEL MKERDRADRG ASNASNLREA
LQSGRPEDRQ LILEKHLSEL LGRGLRLPPA RIERHVPFSN LGMDSLIGLE LRNRIEAALG
ITVPATLLWT YPTVAALSGN LLDILFPNAG ATHAPATERE KSFENDAADL EALLGMTDEQ
KDALLAEKLA QLAQIVGE
//
