ID D4A4X4_RAT Unreviewed; 1194 AA.
AC D4A4X4;
DT 20-APR-2010, integrated into UniProtKB/TrEMBL.
DT 25-MAY-2022, sequence version 3.
DT 27-MAR-2024, entry version 85.
DE RecName: Full=Cingulin {ECO:0000256|ARBA:ARBA00044075};
GN Name=Cgn {ECO:0000313|Ensembl:ENSRNOP00000028440.7,
GN ECO:0000313|RGD:1311992};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116 {ECO:0000313|Ensembl:ENSRNOP00000028440.7, ECO:0000313|Proteomes:UP000002494};
RN [1] {ECO:0000313|Ensembl:ENSRNOP00000028440.7, ECO:0000313|Proteomes:UP000002494}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway {ECO:0000313|Ensembl:ENSRNOP00000028440.7,
RC ECO:0000313|Proteomes:UP000002494};
RX PubMed=15057822; DOI=10.1038/nature02426;
RG Rat Genome Sequencing Project Consortium;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2] {ECO:0007829|PubMed:22673903}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
RN [3] {ECO:0000313|Ensembl:ENSRNOP00000028440.7}
RP IDENTIFICATION.
RC STRAIN=Brown Norway {ECO:0000313|Ensembl:ENSRNOP00000028440.7};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Probably plays a role in the formation and regulation of the
CC tight junction (TJ) paracellular permeability barrier.
CC {ECO:0000256|ARBA:ARBA00043864}.
CC -!- SIMILARITY: Belongs to the cingulin family.
CC {ECO:0000256|ARBA:ARBA00038467}.
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DR AlphaFoldDB; D4A4X4; -.
DR CORUM; D4A4X4; -.
DR IntAct; D4A4X4; 2.
DR STRING; 10116.ENSRNOP00000028440; -.
DR iPTMnet; D4A4X4; -.
DR PhosphoSitePlus; D4A4X4; -.
DR PaxDb; 10116-ENSRNOP00000028440; -.
DR PeptideAtlas; D4A4X4; -.
DR Ensembl; ENSRNOT00000028440.8; ENSRNOP00000028440.7; ENSRNOG00000020952.9.
DR AGR; RGD:1311992; -.
DR RGD; 1311992; Cgn.
DR eggNOG; ENOG502R9EI; Eukaryota.
DR GeneTree; ENSGT00940000162698; -.
DR HOGENOM; CLU_002036_0_0_1; -.
DR InParanoid; D4A4X4; -.
DR OrthoDB; 4226071at2759; -.
DR TreeFam; TF332247; -.
DR Reactome; R-RNO-2173791; TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).
DR Proteomes; UP000002494; Chromosome 2.
DR Bgee; ENSRNOG00000020952; Expressed in jejunum and 18 other cell types or tissues.
DR GO; GO:0043296; C:apical junction complex; ISO:RGD.
DR GO; GO:0005923; C:bicellular tight junction; ISO:RGD.
DR GO; GO:0005903; C:brush border; ISO:RGD.
DR GO; GO:0016459; C:myosin complex; IEA:InterPro.
DR GO; GO:0008017; F:microtubule binding; ISO:RGD.
DR GO; GO:0070830; P:bicellular tight junction assembly; ISO:RGD.
DR GO; GO:0003382; P:epithelial cell morphogenesis; ISO:RGD.
DR GO; GO:0000226; P:microtubule cytoskeleton organization; ISO:RGD.
DR InterPro; IPR002928; Myosin_tail.
DR PANTHER; PTHR46349:SF4; CINGULIN; 1.
DR PANTHER; PTHR46349; CINGULIN-LIKE PROTEIN 1-RELATED; 1.
DR Pfam; PF01576; Myosin_tail_1; 1.
PE 1: Evidence at protein level;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Coiled coil {ECO:0000256|ARBA:ARBA00023054};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Proteomics identification {ECO:0007829|PeptideAtlas:D4A4X4};
KW Reference proteome {ECO:0000313|Proteomes:UP000002494}.
FT DOMAIN 809..1143
FT /note="Myosin tail"
FT /evidence="ECO:0000259|Pfam:PF01576"
FT REGION 24..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 74..122
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 178..258
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 871..901
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1025..1044
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1151..1172
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 83..114
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 195..240
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 241..258
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 871..890
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1194 AA; 136557 MW; C62130401AFF9A49 CRC64;
MMADPRGPVD HGVQIRFITE PEGAAEMGTL RRSGRRPAKD ARASTYGVAV RVQGIAGQPF
VVLNSGEKGA DSFGVQIKGG NNRGSPGALS SDSELPENPY SQVKGYPATS QGSTSDEEPR
DHLNGKLLRS QSQASLTGLA LMSPSNRSTS LLELAPQKTP SVNTIDTAPL SSVDSLINKF
DGQKGSQVRG RTGRRTKTLP QEQQRKRSQS LDSRLSRDTR EDRELQSTNH WARGTKYDNH
MDSSKTLPQK QSPFSSFSRS RQTQDWVLQS FEESREKVQD PAMVIHFPPV PLALFPIFLA
FPFSLLTLAS ILLSSRSADS EASVRRKVSL VLEQMQPLGM VSPASTKALV GQAELTRKME
ELQKKLDEEV KKRQKLEPSR IGLERQLEEK AEECHRLQEL LEKRKGEVQQ SAKELQNMKL
LLGQEEGLRH GLETQVKELQ LKLKHSQNLD PGKESLLKDL LETRELLEEL LEGKQRVEEQ
LRLRERELTA LKGALKEEVA SHDQEVEHVR LQYQRDTEQL RRSMQDATQD HAAVEAERQK
MSSLVRELQK ELEETSEETG HWQNMFQKNK EELRAAKQEL LQLRMEKEEI EEELGEKMEI
LQRDLEQARA STRDTQQVEE LKKELQRTQG ELKELQAERQ SQEVAGRHRN QVLEKQLSVL
RAEADRSRDL EEQNLQLQKT LQQLRQDCEE ASKAKVASET EAMVLGQRQA TVETTLRETQ
EENDEFRRRI LGLEQQLKEA RGLVEGGEAV EARLRDKVHR LEVEKQQLEE ALNVAQEEEG
SLAAAKRALE IRLEEAQRGL ARLGQEQQAL NRALEEEGKQ REALRKSKAE LEEQKRLLNR
TVDRLNKELE QIGDDSKLAL QQLQSQMEDY KEKARKEVAD AQRQAKDWAS EAEKNSGGLS
RLQDELQRLR QALQTSQAER DTARLDKELL AQRLQGLEQE AENKKRSQDD KARQLKSLEE
KVSRLEAELD EEKNTVELLT DRVNRGRDQV DQLRTELMQE RSARQDLECD KISLERQNKD
LKTRLASSEG LQKPSASFSQ LESQNQLLQE RLQAEEREKT VLQSTNRKLE RRVKELSIQI
DDERQHVNDQ KDQLTLRVKA LKRQVDEAEE EIERLDSLRK KAQRELEEQH EVNEQLQARI
KSLEKDSWRK ASRSAAESAL KQEGLSSDEE FDSVYDPSSI ASLLTESNLQ TSSC
//
