ID D4F959_EDWTA Unreviewed; 107 AA.
AC D4F959;
DT 18-MAY-2010, integrated into UniProtKB/TrEMBL.
DT 18-MAY-2010, sequence version 1.
DT 24-JAN-2024, entry version 65.
DE RecName: Full=Iron-binding protein IscA {ECO:0000256|ARBA:ARBA00014591, ECO:0000256|HAMAP-Rule:MF_01429};
DE AltName: Full=Iron-sulfur cluster assembly protein {ECO:0000256|ARBA:ARBA00032050, ECO:0000256|HAMAP-Rule:MF_01429};
GN Name=iscA {ECO:0000256|HAMAP-Rule:MF_01429,
GN ECO:0000313|EMBL:EFE21693.1};
GN ORFNames=EDWATA_03314 {ECO:0000313|EMBL:EFE21693.1};
OS Edwardsiella tarda ATCC 23685.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Hafniaceae; Edwardsiella.
OX NCBI_TaxID=500638 {ECO:0000313|EMBL:EFE21693.1, ECO:0000313|Proteomes:UP000003692};
RN [1] {ECO:0000313|EMBL:EFE21693.1, ECO:0000313|Proteomes:UP000003692}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 23685 {ECO:0000313|EMBL:EFE21693.1,
RC ECO:0000313|Proteomes:UP000003692};
RA Weinstock G., Sodergren E., Clifton S., Fulton L., Fulton B., Courtney L.,
RA Fronick C., Harrison M., Strong C., Farmer C., Delahaunty K., Markovic C.,
RA Hall O., Minx P., Tomlinson C., Mitreva M., Nelson J., Hou S., Wollam A.,
RA Pepin K.H., Johnson M., Bhonagiri V., Zhang X., Suruliraj S., Warren W.,
RA Chinwalla A., Mardis E.R., Wilson R.K.;
RL Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Is able to transfer iron-sulfur clusters to apo-ferredoxin.
CC Multiple cycles of [2Fe2S] cluster formation and transfer are observed,
CC suggesting that IscA acts catalytically. Recruits intracellular free
CC iron so as to provide iron for the assembly of transient iron-sulfur
CC cluster in IscU in the presence of IscS, L-cysteine and the thioredoxin
CC reductase system TrxA/TrxB. {ECO:0000256|HAMAP-Rule:MF_01429}.
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01429};
CC Note=Binds 2 iron ions per dimer. The dimer may bind additional iron
CC ions. {ECO:0000256|HAMAP-Rule:MF_01429};
CC -!- SUBUNIT: Homodimer; may form tetramers and higher multimers.
CC {ECO:0000256|HAMAP-Rule:MF_01429}.
CC -!- SIMILARITY: Belongs to the HesB/IscA family.
CC {ECO:0000256|ARBA:ARBA00006718, ECO:0000256|HAMAP-Rule:MF_01429}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFE21693.1}.
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DR EMBL; ADGK01000270; EFE21693.1; -; Genomic_DNA.
DR RefSeq; WP_005289574.1; NZ_GG739633.1.
DR AlphaFoldDB; D4F959; -.
DR GeneID; 72529592; -.
DR HOGENOM; CLU_069054_5_1_6; -.
DR Proteomes; UP000003692; Unassembled WGS sequence.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProt.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:UniProtKB-UniRule.
DR Gene3D; 2.60.300.12; HesB-like domain; 1.
DR HAMAP; MF_01429; Fe_S_insert_IscA; 1.
DR InterPro; IPR000361; FeS_biogenesis.
DR InterPro; IPR016092; FeS_cluster_insertion.
DR InterPro; IPR017870; FeS_cluster_insertion_CS.
DR InterPro; IPR035903; HesB-like_dom_sf.
DR InterPro; IPR011302; IscA_proteobacteria.
DR NCBIfam; TIGR00049; iron-sulfur cluster assembly accessory protein; 1.
DR NCBIfam; TIGR02011; IscA; 1.
DR PANTHER; PTHR10072:SF41; IRON-SULFUR CLUSTER ASSEMBLY 1 HOMOLOG, MITOCHONDRIAL; 1.
DR PANTHER; PTHR10072; IRON-SULFUR CLUSTER ASSEMBLY PROTEIN; 1.
DR Pfam; PF01521; Fe-S_biosyn; 1.
DR SUPFAM; SSF89360; HesB-like domain; 1.
DR PROSITE; PS01152; HESB; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|HAMAP-Rule:MF_01429};
KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_01429}.
FT DOMAIN 1..103
FT /note="FeS cluster biogenesis"
FT /evidence="ECO:0000259|Pfam:PF01521"
FT BINDING 35
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01429"
FT BINDING 99
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01429"
FT BINDING 101
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01429"
SQ SEQUENCE 107 AA; 11607 MW; B8FFC0A04B7904E4 CRC64;
MSITMSDSAA KRVQTFLTNR GKGLGLRLGV KTSGCSGMAY VLEFVDVLNE DDVVFEDKGV
KVIVDGKSLV YLDGTELDFV KEGLNEGFKF NNPNVKNECG CGESFNV
//
