ID D4GG32_PANAM Unreviewed; 575 AA.
AC D4GG32;
DT 18-MAY-2010, integrated into UniProtKB/TrEMBL.
DT 18-MAY-2010, sequence version 1.
DT 27-MAR-2024, entry version 91.
DE RecName: Full=K(+)/H(+) antiporter NhaP2 {ECO:0000256|HAMAP-Rule:MF_01075};
DE AltName: Full=Potassium/proton antiporter NhaP2 {ECO:0000256|HAMAP-Rule:MF_01075};
GN Name=nhaP2 {ECO:0000256|HAMAP-Rule:MF_01075};
GN Synonyms=cvrA {ECO:0000313|EMBL:ADD77283.1};
GN OrderedLocusNames=PANA_2116 {ECO:0000313|EMBL:ADD77283.1};
OS Pantoea ananatis (strain LMG 20103).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Pantoea.
OX NCBI_TaxID=706191 {ECO:0000313|EMBL:ADD77283.1, ECO:0000313|Proteomes:UP000001702};
RN [1] {ECO:0000313|EMBL:ADD77283.1, ECO:0000313|Proteomes:UP000001702}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LMG 20103 {ECO:0000313|EMBL:ADD77283.1,
RC ECO:0000313|Proteomes:UP000001702};
RX PubMed=20348253; DOI=10.1128/JB.00060-10;
RA De Maayer P., Chan W.Y., Venter S.N., Toth I.K., Birch P.R., Joubert F.,
RA Coutinho T.A.;
RT "Genome sequence of Pantoea ananatis LMG20103, the causative agent of
RT Eucalyptus blight and dieback.";
RL J. Bacteriol. 192:2936-2937(2010).
CC -!- FUNCTION: K(+)/H(+) antiporter that extrudes potassium in exchange for
CC external protons and maintains the internal concentration of potassium
CC under toxic levels. {ECO:0000256|HAMAP-Rule:MF_01075}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(out) + K(+)(in) = H(+)(in) + K(+)(out);
CC Xref=Rhea:RHEA:29467, ChEBI:CHEBI:15378, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01075};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|HAMAP-
CC Rule:MF_01075}; Multi-pass membrane protein {ECO:0000256|HAMAP-
CC Rule:MF_01075}. Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-pass
CC membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1)
CC transporter (TC 2.A.36) family. NhaP2 subfamily. {ECO:0000256|HAMAP-
CC Rule:MF_01075}.
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DR EMBL; CP001875; ADD77283.1; -; Genomic_DNA.
DR RefSeq; WP_013025993.1; NC_013956.2.
DR AlphaFoldDB; D4GG32; -.
DR STRING; 706191.PANA_2116; -.
DR GeneID; 57268040; -.
DR KEGG; pam:PANA_2116; -.
DR eggNOG; COG3263; Bacteria.
DR HOGENOM; CLU_005912_9_2_6; -.
DR Proteomes; UP000001702; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0015386; F:potassium:proton antiporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006884; P:cell volume homeostasis; IEA:InterPro.
DR Gene3D; 1.20.1530.20; -; 1.
DR Gene3D; 3.30.465.10; -; 1.
DR Gene3D; 3.30.70.1450; Regulator of K+ conductance, C-terminal domain; 1.
DR HAMAP; MF_01075; NhaP2; 1.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR036318; FAD-bd_PCMH-like_sf.
DR InterPro; IPR016169; FAD-bd_PCMH_sub2.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR InterPro; IPR023729; NhaP2.
DR InterPro; IPR006037; RCK_C.
DR InterPro; IPR036721; RCK_C_sf.
DR InterPro; IPR005170; Transptr-assoc_dom.
DR PANTHER; PTHR32507:SF7; K(+)_H(+) ANTIPORTER NHAP2; 1.
DR PANTHER; PTHR32507; NA(+)/H(+) ANTIPORTER 1; 1.
DR Pfam; PF03471; CorC_HlyC; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
DR Pfam; PF02080; TrkA_C; 1.
DR SMART; SM01091; CorC_HlyC; 1.
DR SUPFAM; SSF56176; FAD-binding/transporter-associated domain-like; 1.
DR SUPFAM; SSF116726; TrkA C-terminal domain-like; 1.
DR PROSITE; PS51202; RCK_C; 1.
PE 3: Inferred from homology;
KW Antiport {ECO:0000256|ARBA:ARBA00022449, ECO:0000256|HAMAP-Rule:MF_01075};
KW Cell inner membrane {ECO:0000256|HAMAP-Rule:MF_01075};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475, ECO:0000256|HAMAP-
KW Rule:MF_01075};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065, ECO:0000256|HAMAP-
KW Rule:MF_01075};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|HAMAP-Rule:MF_01075};
KW Potassium {ECO:0000256|HAMAP-Rule:MF_01075};
KW Potassium transport {ECO:0000256|ARBA:ARBA00022538, ECO:0000256|HAMAP-
KW Rule:MF_01075}; Reference proteome {ECO:0000313|Proteomes:UP000001702};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|HAMAP-
KW Rule:MF_01075};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, ECO:0000256|HAMAP-
KW Rule:MF_01075};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|HAMAP-Rule:MF_01075}.
FT TRANSMEM 6..23
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 30..54
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 60..77
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 89..115
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 185..209
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 221..252
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 273..292
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 298..323
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 335..355
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 361..378
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT DOMAIN 403..485
FT /note="RCK C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51202"
SQ SEQUENCE 575 AA; 61962 MW; 044EDD70EC93EEFA CRC64;
MNHTIVYSLF IIGSVLVASS ILLSSFSSRL GIPILVIFLA LGMLAGVDGI GGIAFDNYPA
AYLISNLALA IILLDGGMRT KASSFKVALG PALSLATVGV MITAGLTGVA AAWLFRLDMM
QGFLIGAIIG STDAAAVFSL LGDKGLNERV SSTLEIESGS NDPMAVFLTI TLIEMIEQGK
TGLDWMFLVH LIQQFGLGIV LGLGGGWALQ QLINRINLAP GLYPLLALSG GILVFALTTV
LEGSGILAVY LCGFLLGNSP IRNRFGILQT FDGMAWLSQI GMFIVLGLLV TPSDLWHIAV
PAMILSLWLI LVARPLSVFI GLLPFRGFTG RERIFISWVG LRGAVPIILA VFPMMAGLEN
ASLYFNIAFF VVLVSLMLQG TSLGYAAKKA KVVVPPTASP VSRIGLDIHP ENPWEQFVYQ
LSADKWCVGA ALRDLQMPRE TRIAALFRDN VLMHPNGATR LKENDVICVI GRERDLPALG
KMFSQSPPVA LDQRFFGDFI LDADARLNDV AQIYGLELDE QTNAQQSLGQ LMLGMLGGTP
VVGDQVEWNQ LTWTVAEKED NQIVKIGVRP SEDKE
//
