ID D4JGC7_9FIRM Unreviewed; 300 AA.
AC D4JGC7;
DT 18-MAY-2010, integrated into UniProtKB/TrEMBL.
DT 18-MAY-2010, sequence version 1.
DT 24-JAN-2024, entry version 62.
DE SubName: Full=Glycyl-radical enzyme activating protein family {ECO:0000313|EMBL:CBK89249.1};
DE EC=1.97.1.4 {ECO:0000313|EMBL:CBK89249.1};
GN ORFNames=EC1_20860 {ECO:0000313|EMBL:CBK89249.1};
OS Faecalitalea cylindroides T2-87.
OC Bacteria; Bacillota; Erysipelotrichia; Erysipelotrichales;
OC Erysipelotrichaceae; Faecalitalea.
OX NCBI_TaxID=717960 {ECO:0000313|EMBL:CBK89249.1, ECO:0000313|Proteomes:UP000008801};
RN [1] {ECO:0000313|EMBL:CBK89249.1, ECO:0000313|Proteomes:UP000008801}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T2-87 {ECO:0000313|EMBL:CBK89249.1,
RC ECO:0000313|Proteomes:UP000008801};
RG metaHIT consortium -- http://www.metahit.eu/;
RA Pajon A., Turner K., Parkhill J., Duncan S., Flint H.;
RT "The genome sequence of Eubacterium cylindroides T2-87.";
RL Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:CBK89249.1, ECO:0000313|Proteomes:UP000008801}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T2-87 {ECO:0000313|EMBL:CBK89249.1,
RC ECO:0000313|Proteomes:UP000008801};
RA Pajon A.;
RL Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the organic radical-activating enzymes family.
CC {ECO:0000256|ARBA:ARBA00009777}.
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DR EMBL; FP929041; CBK89249.1; -; Genomic_DNA.
DR RefSeq; WP_015536460.1; NZ_JAQLXG010000002.1.
DR AlphaFoldDB; D4JGC7; -.
DR STRING; 717960.EC1_20860; -.
DR GeneID; 79876027; -.
DR KEGG; euc:EC1_20860; -.
DR PATRIC; fig|717960.3.peg.1548; -.
DR HOGENOM; CLU_058969_0_0_9; -.
DR Proteomes; UP000008801; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0043365; F:[formate-C-acetyltransferase]-activating enzyme activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.30.70.20; -; 1.
DR Gene3D; 3.80.30.10; pyruvate-formate lyase- activating enzyme; 1.
DR InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR InterPro; IPR040074; BssD/PflA/YjjW.
DR InterPro; IPR034457; Organic_radical-activating.
DR InterPro; IPR012839; Organic_radical_activase.
DR InterPro; IPR001989; Radical_activat_CS.
DR InterPro; IPR007197; rSAM.
DR NCBIfam; TIGR02494; PFLE_PFLC; 1.
DR PANTHER; PTHR30352:SF14; PYRUVATE FORMATE-LYASE 3-ACTIVATING ENZYME-RELATED; 1.
DR PANTHER; PTHR30352; PYRUVATE FORMATE-LYASE-ACTIVATING ENZYME; 1.
DR Pfam; PF12838; Fer4_7; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR PIRSF; PIRSF000371; PFL_act_enz; 1.
DR SFLD; SFLDG01118; activating_enzymes__group_2; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SUPFAM; SSF54862; 4Fe-4S ferredoxins; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS00198; 4FE4S_FER_1; 2.
DR PROSITE; PS51379; 4FE4S_FER_2; 2.
DR PROSITE; PS01087; RADICAL_ACTIVATING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000313|EMBL:CBK89249.1};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 14..295
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
FT DOMAIN 45..74
FT /note="4Fe-4S ferredoxin-type"
FT /evidence="ECO:0000259|PROSITE:PS51379"
FT DOMAIN 75..103
FT /note="4Fe-4S ferredoxin-type"
FT /evidence="ECO:0000259|PROSITE:PS51379"
SQ SEQUENCE 300 AA; 33764 MW; BF6AD685DC8DEF61 CRC64;
MKANIMEIER FAIHDGPGIR SVVFLQGCPL RCPWCANPES QEIKPQLMYN EEKCVRCGTC
VRNCPQHAIK IENFKLVFDR DACIHCKTCE NNCPAGAINF IGDAVSIDDI VTEVLKDKPF
YEESNGGVTI SGGEPFVQYN ALKALLNKFF EEDISVAVET TGDTHWKNIE DLIPYIDYFL
FDLKFPTANR ISLVTKGNGE RILENLKKLA SKAPEKITLR VPVIPNYNYD ERTIRKIIDI
GAELKVSQIH LLPYHTLGVS KYDQLGKEYD LKGSKMLSKE DLKDLVDYGN SKGIHTQAGG
//
